Ambrysus circumcinctus Montandon

Reynoso-Velasco, Daniel, Sites, Robert W. & Novelo-Gutiérrez, Rodolfo, 2016, The Ambrysus Stål (Heteroptera: Naucoridae: Cryphocricinae) of Mexico: Revision of the subgenus Syncollus La Rivers, Zootaxa 4126 (1) : -

Ambrysus circumcinctus Montandon

( Figs. 7–8 )

A. circumcinctus Montandon 1910 : Bull. Soc. Stiinte 19: 442–444 (original description).

A. circumcinctus: Hungerford 1919 , Univ. Kans. Sci. Bull. 21: 201–202 (translation of the original description).

A. caliginosus Usinger 1946 : Univ. Kans. Sci. Bull. 31: 190–191 (original description).

A. circumcinctus: La Rivers 1951 , Univ. Calif. Publ. Entomol. 8: 292–295 (supplemental description).

A. circumcinctus caliginosus Usinger : La Rivers 1953, Univ. Kans. Sci. Bull. 35: 1290–1292 (considered a subspecies and description of the subspecies), (new synonym).

A. circumcinctus concavus La Rivers 1967 : Biol. Soc. Nevada Occas. Pap. 14: 3–4 (subspecies description), (new synonym).

A. circumcinctus extremus La Rivers 1967 : Biol. Soc. Nevada Occas. Pap. 14: 3 (subspecies description), (new synonym).

Diagnosis. This species can be distinguished from congeners based on the shape and length of the AGP, as well as the shape of the pseudoparameres. In general, the AGP presents the characteristics described by La Rivers (1951): moderately long, parallel-sided, noncapitate, blunt at tip, and uniformly curved to the right at approximately 45 degree angle ( Fig. 7 B). The pseudoparameres are relatively long, rounded and slightly asymmetrical, with the right pseudoparamere moderately narrower toward the apex; with a pile of fine setae mostly on the mesal margins ( Fig. 7 C). The parameres are symmetrical, longer than wide, with the mesal margin straight, and with a row of long setae emanating from the middle of the dorsal surface ( Fig. 7 D). The left ventral lobe of the endosoma is lightly sclerotized ( Fig. 7 E). The posterior margin of the SGP is highly variable, but in general it has a shallow concavity with a central notched lobe, and rounded to squared posterolateral corners ( Fig. 7 F).

Discussion. This species originally was described from a single female specimen from Kerrville, Texas ( Montandon 1910) and later assigned as the type species of the subgenus Syncollus by La Rivers (1965). Usinger (1946) described the species A. caliginosus from central Mexico, which was later considered by La Rivers (1953) to be a subspecies of A. circumcinctus based on the relatively larger size and darker coloration. Later, La Rivers (1967) described two more subspecies: Ambrysus c. extremus from Honduras and Costa Rica with the diagnostic feature of the median lobe in the center of the posterior margin of the SGP conspicuously produced beyond the base of the concavity; and A. c. concavus , described from central Mexico, with a deeper concavity in the posterior margin of the SGP and the median lobe barely visible. In contrast, the nominal subspecies had a nearly straight posterior margin of the SGP with a short median lobe. The characteristics of the AGP reported for A. circumcinctus ( La Rivers 1951) were the same reported to be present in A. c. concavus and A. c. caliginosus , whereas A. c. extremus had a distally expanded AGP ( La Rivers 1967).

Considering that a subspecies is a geographical variety ( Mayr 1982) with morphological uniformity in one or a few diagnostic characters ( Wilson & Brown 1953), the four subspecies of A. circumcinctus should be identifiable and associated to a particular geographic area based on the shape of the posterior margin of the subgenital plate ( La Rivers 1967). This is not the case in part because the subspecies described by La Rivers were based on few specimens from disparate localities and he was not able to assess the variation in subgenital plate shape. We have studied ca. 3,000 specimens from approximately 300 localities from the U.S. to Honduras; thus, this massive amount of material has enabled us to assess intraspecific phenotypic plasticity of this feature. What La Rivers considered a stable and consistent feature was an artifact of the small number of specimens and localities used to describe the subspecies.

At various localities in the range of A. circumcinctus , populations exhibit the characteristics of more than one described subspecies. In one example of many, at site L- 1350 in Chiapas (southern Mexico) variation in subgenital plate shape includes individuals consistent with that of both A. c. extremus and A. c. circumcinctus . The latter subspecies purportedly occurs only in the United States and northern Mexico. Because we have examples of more than one subspecific morphological form occurring in various populations and occurrence of the morphological forms does not conform to the latitudinal geographic organization suggested by La Rivers (1967), we consider these subspecies to be consubspecific. As such, we propose A. c. caliginosus , A. c. concavus , and A. c. extremus to be junior synonyms of A. c. circumcinctus .

Variation. The coloration of the hemelytra reported for A. circumcinctus was uniformly brownish black with embolia predominantly light yellow, and dark only posteriorly ( La Rivers 1951). The coloration of specimens collected in the southern United States was lighter when compared with specimens from central Mexico ( La Rivers 1953) and present a conspicuous, light brown triangular mark at the base of the posterior margin of the corium. The coloration of the hemelytra of specimens collected throughout Mexico is solid dark brown with the triangular mark inconspicuous and light brown to reddish brown ( Fig. 7 A), although some specimens collected in Jalisco, Michoacán, and Nayarit posses a conspicuous triangular mark similar to that present in specimens from the southern United States. A similar mark also is present in specimens of A. baeus distributed in western Mexico. Minor variation in the length of the AGP also was observed. The posterior margin of the SGP showed a high degree of phenotypic plasticity that included the posterior margin with a: a) shallow concavity with central notched lobe, b) shallow concavity with basally constricted central notched lobe, c) deeper concavity with inconspicuous central notched lobe, and d) posterior margin almost straight (without concavity) with inconspicuous central notched lobe.

Type locality. United States: Texas: Kerr Co., Kerrville.

Repository. Holotype is housed at USNM.

Distribution. This species has the largest range among species in this subgenus in North America. It is distributed from the southern United States south to Costa Rica. In Mexico, it is widely distributed with representatives in most of the biogeographic provinces from the Nearctic and Neotropical regions, except the Californian and Baja Californian provinces where the species most likely does not occur ( Fig. 8 ). It also is present in the provinces of the Mexican Transition Zone; however, at high elevations it is replaced by other closely related species (i.e., A. pygmaeus , A. totonacus n. sp., A. xico n. sp.). We found A. circumcinctus and A. baeus occupying the same habitat at various localities within the ranges of both species (L-1342, L-1371, L-1488, L-1661, L-1801, L-1802, L-1891, L-1900), and with A. totonacus n. sp. in the states of Hidalgo (L-1522) and Veracruz (L-1290, L- 1296, L-1549).

Published records. Belize: Cayo, Orange Walk ( Sites & Reynoso-Velasco 2015, Sites & Shepard 2015). Costa Rica: San José ( La Rivers 1967, 1971; Herrera 2013). Guatemala ( Herrera 2013). Honduras: Atlántida, El Paraíso, Olancho ( La Rivers 1967, 1971; Herrera 2013). Mexico: Chiapas, Estado de México, Nuevo León, San Luis Potosí, Sinaloa ( La Rivers 1953, 1965, 1967, 1971; Davis 1986; Lee 1991; Herrera 2013). United States: Arizona, New Mexico, Texas ( Montandon 1910; La Rivers 1951, 1953, 1965, 1967, 1971; Davis 1986; Herrera 2013).

Type material examined. HOLOTYPE ♀: UNITED STATES: [Texas: Kerr Co.], Kerrville, vi.19.07 TX / F. C. Pratt Collector / From Coll. U.S. N.M. / 0 52 / Ambrysus circumcinctus Montandon Type 1909 / type No. 54270 U.S. N.M. PARATYPES: MÉXICO: MÉXICO: Tejupilco, Mex., Temescaltepec [Temascaltepec], VI 18 33 / H. E. Hinton, R. L. Usinger Collectors / PARATYPE Ambrysus caligimosus R. L. Usinger (4♀ EMEC); same but / Ambrysus circumcinctus caliginosus determined by Ira La Rivers '50 (1♀ EMEC); Tejupilco, Mex., Temescaltepec [Temascaltepec], VI 21 33 / H. E. Hinton, R. L. Usinger / PARATYPE Ambrysus caliginosus R. L. Usinger (1♂ EMEC).

Material examined. MÉXICO: CHIAPAS: Mpio. Amatengo de la Frontera, Barrio Sabinalito, Río Agua Caliente, 15° 25' 0.1'' N, 92° 08' 33.7'' W, 942 m, 31-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1898 (1♀ UMC); Mpio. Ángel Albino Corzo, Jaltenango, Río Jaltenango (Río Lagartero), 15º 52' 33.7'' N, 92º 43' 53.4'' W, 605 m, 06-Jan-2012, DRV coll., L-1313 (24³, 20♀ UMC); same but 07-Jan-2012, L-1314 (46³, 45♀ UMC); Mpio. Ángel Albino Corzo, km 30 carr. Jaltenango-Siltepec, Plan de Ayutla, Río El Plan de Ayutla, 15º 42' 23.6'' N, 92º 34' 43.7'' W, 846 m, 19-May-2012, DRV & PRH colls., L-1346 (5³, 4♀ UMC); Mpio. Ángel Albino Corzo, 1 km S of Finca Prusia, Río Prusia, 15º 43' 23.3'' N, 92º 47' 46.5'' W, 1010 m, 18-May-2012, DRV & PRH colls., L- 1345 (1³ UMC); Mpio. Benemérito de las Américas, Flor de Cacao, tributario del Río Salinas, 16° 09' 24.4'' N, 90° 29' 38'' W, 140 m, 30-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1891 (³1 UMC); Mpio. Frontera Comalapa, El Caracol, 8 km S of Frontera Comalapa, Río Grijalva, 15° 36' 07.0'' N, 92° 09' 14.5'' W, 677 m, 31- Mar -2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1897 (1³, 1♀ UMC); Mpio. Huixtla, Huixtla, Río Huixtla, 15° 08' 59.9'' N, 92° 27' 04.6'' W, 67 m, 31-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1899 (1♂, 4♀ UMC); same but 01-Apr-2015, L-1901 (6³, 3♀ UMC); Mpio. Ixtapa, Ixtapa, 16º 48' 43.7'' N, 92º 54' 30'' W, 1030 m, 17-May-2012, DRV & PRH colls., L-1341 (1♀ UMC); Mpio. La Concordia, El Altillo, Río El Dorado, 16º 06' 27.5'' N, 92º 55' 29.3'' W, 524 m, 18-May-2012, DRV & PRH colls., L-1342 (1♀ UMC); Mpio. La Concordia, Pte. Independencia, 16º 04' 25.2'' N, 92º 49' 45.3'' W, 547 m, 07-Jan-2012, DRV coll., L-1317 (43³, 32♀ UMC); Mpio. Maravilla Tenejapa, Ixcán, Pte. Ixcán, Río Ixcán, 16° 04' 33.6'' N, 91° 04' 12.8'' W, 175 m, 30-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1896 (7³, 9♀ UMC); [Mpio. Metapa], Metapa [de Domínguez], Río Suchiate, 25-III-85, M. Vértiz (1♀, CNIN); same but / Ambrysus circumcinctus Montd. R. W. Sites det. (1♀ CNIN); Mpio. Monte Cristo de Guerrero, El Paraíso, 15º 39' 28.1'' N, 92º 36' 18.7 '' W, 864 m, 19-May-2012, DRV & PRH colls., L-1347 (1³ UMC); Mpio. Ocosingo, Balneario Jataté, Río Jataté, 16° 52' 20.1'' N, 92° 02' 18.9'' W, 861 m, 28-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1887 (1♀ UMC); Mpio. Palenque, Ángel Albino Corzo, Balneario, Río Chocolja, 17° 17' 32'' N, 91° 37' 49.5'' W, 119 m, 29-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1888 (17³, 13♀ UMC); Mpio. Pichucalco, Pte. Rosita, 4 km S of Pichucalco and 800 m S of Río de Janeiro, 17° 29' 07.2'' N, 93° 06' 13.5'' W, 44 m, 27-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls. L-1883 (2♀ UMC); Mpio. Siltepec, km 72 carr. Jaltenango-Siltepec, nr. Nuevo Galeana, 15º 35' 54.9'' N, 92º 32' 28.9'' W, 933 m, 19-May-2012, DRV & PRH colls., L-1349 (36³, 25♀ UMC); Mpio. Siltepec, km 92 carr. Jaltenango- Siltepec, Campo Aéreo, Río Bravo, 15º 35' 17.9'' N, 92º 28' 02.3'' W, 1068 m, 19-May-2012, DRV & PRH colls., L- 1350 (32³, 31♀ UMC); Mpio. Solosuchiapa, El Beneficio, Río La Sierra, 17° 21' 01.1'' N, 93° 00' 21.1'' W, 303 m, 27-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1884 (1³, 3♀ UMC); Mpio. Solosuchiapa, Solosuchiapa, Río La Sierra, 17º 25' 40.5'' N, 93º 01' 35.7'' W, 122 m, 17-May-2012, DRV & PRH colls., L-1339 (2³ UMC); [Mpio. Tapachula], Tapachula, Puente Colorado, 31-VIII-85, H. Velasco (1♀ CNIN); [Mpio. Tecpatán], Chintul [El Progreso], 6-III- '88, R. Barba, E. Barrera, A. Cadena (4³, 2♀ CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (1³, 1♀ CNIN); Mpio. Villa Comaltitlán, 1.36 km SE of Villa Comaltitlán, 15° 12' 45.8'' N, 92° 33' 46.1'' W, 48 m, 31-Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L- 1900 (8³, 8♀ UMC); same locality information but / William D. Shepard leg. (3³, 4♀ UMC). CHIHUAHUA: Mpio. Balleza, Balleza, Río de Balleza, 26° 55' 20.8'' N, 106° 19' 50.1'' W, 1574 m, 30-Dec-2013, DRV & PRH colls., L-1703 (15³, 21♀ UMC); [Mpio. Camargo], Camargo, Río Conchos, 17 August 1952, J. D. Lattin Cal. Acad. Sci. Coll. / Ira La Rivers collection bequeathed to the California Academy of Sciences - 1978 (♂21, 15♀ CAS); [Mpio. Camargo], Camargo, CL1005, 19 April 1964, J. T. & M. S. Polhemus (1³ SEMC); same but / Ambrysus circumcinctus Mont. Det. J.T. Polhemus (1♀ SEMC); [Mpio. Meoqui], Meoqui, Río San Pedro, 20 August 1952, J. D. Lattin Cal. Acad. Sci. Coll. / Ira La Rivers collection bequeathed to the California Academy of Sciences - 1978 (3♂, 3♀ CAS). COAHUILA: Mpio. Cuatrociénegas, La Poza Azul, Arroyo La Puerta Colorada, 26° 55' 48.3'' N, 102° 07' 41.5'' W, 738m, 16-May-2014, DRV & PRH colls., L-1763 (2³, 1♀ UMC); Mpio. Cuatrociénegas, San Juan de Boquillas, 26° 59' 58.0'' N, 101° 53' 35.8'' W, 697m, 17-May-2014, DRV & PRH colls., L-1764 (6³, 1♀ UMC); [Mpio. Jimenez], Río San Francisco, 27 km S of Cd. Acuña, April 0 1, 1974, R. R. & F. M. Miller (1³ UMMZ); Mpio. Sabinas, Sabinas (at El Vado), Río Sabinas, 27° 50' 19.5'' N, 101° 07' 20.2'' W, 340m, 17-May-2014, DRV & PRH colls., L-1765 (1³, 2♀ UMC); Mpio. Villa de Juaréz, Sección Veintidos Cero, Río Salado, 27° 26' 36.0'' N, 100° 28' 38.9'' W, 226m, 18-May-2014, DRV & PRH colls., L-1768 (32♂, 21♀ UMC); Mpio. Zaragoza, 25 km N of Zaragoza, Pte. Río San Rodrigo, Río San Rodrigo, 28° 43' 50.5'' N, 100° 54' 45.4'' W, 329m, 17-May-2014, DRV & PRH colls., L-1766 (11³, 24 ♀ UMC); Río Salado de Los Nadadores, at El Carino, 5-IV-1961, R. R. Miller et al., UMMZ Fish M61-43 (1♀ UMMZ). DURANGO: Mpio. Guanaceví, El Potrero Viejo, Río Sextín, 25° 56' 28.7'' N, 105° 49' 43.0'' W, 1931 m, 02-Jan-2014, DRV & PRH colls., L-1713 (3♀ UMC); Mpio. Nombre de Dios, km 239 carr. Vicente Guerrero-Durango, jct to El Venado, Pte. Melones, Río Mezquital (Río Durango), 23° 52' 17.3'' N, 104° 16' 04.7'' W, 1731 m, 29-Jun-2013, DRV & PRH colls., L-1591 (3³, 4♀ UMC); same but 04-Jan-2014, L-1721 (4³, 8♀ UMC); [Mpio. Peñón Blanco], Peñón Blanco (Belem), 10-VII-85, E. Barrera (1³, 1♀ CNIN); [Mpio. Peñón Blanco], Peñón Blanco, 10-VII-85, J. Bueno (7³, 3♀ CNIN); same but / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (2³, 1♀ CNIN); Mpio. San Bernardo, San Bernardo, Río Sextín (Río Nazas), 26° 00' 05.0'' N, 105° 30' 17.6'' W, 1607 m, 01-Jan-2014, DRV & PRH colls., L-1711 (13³, 9♀ UMC); Mpio. Santa Maria de Tepehuanes, Los Corrales, 25° 12' 13.4'' N, 105° 34' 22.6'' W, 1726 m, 03-Jan-2014, DRV & PRH colls., L-1717 (20³, 26♀ UMC); Mpio. Villa de Ocampo, Villa de Ocampo, Río Florido, 26° 26' 07.2'' N, 105° 30' 24.3'' W, 1719 m, 01-Jan-2014, DRV & PRH colls., L-1710 (1♂, 1♀ UMC); 33 mi W Otinapa, Río Arenales, 12 July 1952, J. D. Lattin Cal. Acad. Sci. Coll. / Ira La Rivers collection bequeathed to the California Academy of Sciences - 1978 (2♂ CAS). GUERRERO: Mpio. Atenango del Río, Atenango del Río, Río Amacuzac, 18º 06' 01.5'' N, 99º 06' 27.8'' W, 631 m, 30-Dec-2012, DRV & PRH colls., L-1499 (5³, 7♀ UMC); Mpio. Copalillo, Papalutla, Río Balsas (Río Papalutla), 18º 01' 27'' N, 98º 54' 09.3'' W, 627 m, 30-Dec-2012, DRV & PRH colls., L-1500 (11³, 6♀ UMC); Mpio. Teloloapan, Oxtotitlán, Río Oxtotitlán, 18º 11' 35.7'' N, 99º 56' 24.3'' W, 1009 m, 29-Dec-2012, DRV & PRH colls., L-1498 (1♀ UMC); [Mpio. Tepecoacuilco de Trujano], Apan, near San Agustín Oapan, at Balsas River, 15 November 2008 / 17º 56' 43'' N, 99º 26' 0 9 W, 505 m, J. D. Amit & A. Domínguez (3³, 4♀ UMC); [Mpio. Tepecoacuilco de Trujano], 17º 57' 14'' N, 99º 26' 22'' W, Balsas River, S[an] Ag[ustín] Oapan, 1650 ft, 6 June 2006, #302, coll. J. Amith (2♂ UMC). HIDALGO: Mpio. Atlapexco, Atlaltipa Huitzotlaco, Pte. Atlaltipa, 21° 02' 12.3'' N, 98° 22' 24.7'' W, 158m, 27- May-2014, DRV & PRH colls., L-1796 (1♀ UMC); Mpio. Huejutla de Reyes, Huitzquilititla, 21° 06' 39.9'' N, 98° 27' 24.4'' W, 203m, 27-May-2014, DRV & PRH colls., L-1795 (2♀ UMC); Mpio. Molango Escamilla, Malila, Río Malila, 20° 43' 43.8'' N, 98° 43' 14.2'' W, 1386 m, 05-Jun-2013, DRV, Sites, Shepard & PRH colls., L-1518 (1♀ UMC); Mpio. San Felipe Orizatlán, Huextetitla, 21° 09' 44.4'' N, 98° 33' 26.6'' W, 154 m, 07-Jun-2013, DRV, Sites, Shepard & PRH colls., L-1525 (1♀ UMC); Mpio. San Felipe Orizatlán, Orizatlán, Pte. Tuntitlán, Río Tuntitlán, 21° 10' 03.4'' N, 98° 36' 58.4'' W, 175 m, 07-Jun-2013, DRV, Sites, Shepard & PRH colls., L-1527 (2³ UMC); Mpio. San Felipe Orizatlán, San Felipe Orizatlán, Río San Pedro, 21° 10' 13.6'' N, 98° 35' 46.8'' W, 173 m, 07-Jun-2013, DRV, Sites, Shepard & PRH colls., L-1526 (18³, 27♀ UMC); Mpio. Tlanchinol, 3.4 km W of Chalchocotipa, road to Santa Maria Catzotipan (west of Hwy 105, km 3+400), 21° 02' 36.4'' N, 98º 36' 00.8'' W, 528 m, 06-Jun-2013, DRV, Sites, Shepard & PRH colls., L-1522 (2³, 5♀ UMC); Mpio. Xochiatipan, Nuevo Acatepec, Pte. Garces Principal, Río El Encinal, 20° 56' 23.7'' N, 98° 16' 54.9'' W, 201m, 27-May-2014, DRV & PRH colls., L-1797 (7♂, 4♀ UMC). JALISCO: Estanzuela, Puente La Estanzuela, W of Ameca, 13 Jan. 2005, C. B. Barr / 20º 31.49' N, 104º 20.16' W, elevation 4580 ft. (1♀ EMEC); Estanzuela, 13 I 2005, 4580', unnamed stream / William D. Shepard, leg. (1♀ EMEC); Hwy 200 @ km 84.5 6 I 2005, 100', Río San Nicolas / William D. Shepard, leg. (1♀ EMEC); Mpio. Atenguillo, Los Volcanes, Río Atenguillo, 20° 19' 16.5'' N, 104° 31' 41.1'' W, 1459 m, 27-Jun-2013, DRV & PRH colls., L-1582 (5³, 5♀ UMC); [Mpio. La Huerta], SE of Chamela, 6 I 2005, 180', Río Cuitzmala / William D. Shepard, leg. (1♀ EMEC); Mpio. Puerto Vallarta, Las Palmas, 235 m, 20º 49.515' N, 105º 05.367' W, 16 March 2014, colls: J. A. Gómez, S. Smith & R. Novelo (1³ IEXA); Mpio. San Sebastian del Oeste, Pte. Los Arrayanes, Arroyo Los Arrayanes, 20° 49' 44.5'' N, 105° 00' 45.1'' W, 337 m, 26-Jun-2013, DRV & PRH colls., L-1579 (1♂, 1♀ UMC); [Mpio. Villa Purificación], Río El Amborín, 6.6 rd, km SE Villa Purificación, 19º 38.81' N, 104º 33.92' W, 8 Jan. 2005, C. B. Barr (2♀ UMC; 1³, 1♀ EMEC); [Mpio. Villa Purificación], 6.6 km SE Villa Purificación, 8 I 2005, Puente El Amborin / William D. Shepard leg. (9³, 5♀ EMEC); Río de Tepospisaloya, ca. 6 km N Unión de Tula, 9.2 km W Tacotán, 10 Jan. 2005, C. B. Barr / 20º 03.86' N, 104º 20.91' W, elevation 1250 ft. (1³, 1♀ EMEC); Pte. San Buenaventura at Rd. 513, 8.5 rd. km W of Tonaya, [Río Ferreria], 11 Jan. 2005, coll. C. B. Barr / 19º 47.12' N, 104º 02.71' W, elevation 2360 ft. (1♀ EMEC); 2.4 km SE Purificación, 8 I 2005, 1280', Río Purificación / William D. Shepard leg. (1³ EMEC); 8.5 km W Tonaya, 11 I 2005, 2360', Puente San Buenavista [San Buenaventura], [Río Ferreria] / William D. Shepard leg. (5³, 4♀ EMEC); 9.2 km W Tacotán, 10 I 2005, 1250', Río de Tepospisaloya / William D. Shepard leg. (1♂, 2♀ EMEC). MÉXICO: Mpio. Amatepec, Las Adjuntas, Río Sn. Felipe, 05-May-2008, R. Novelo & K. Cuevas cols. (1♀ IEXA); [Mpio.] Amatepec, Sn. Felipe de Jesús (1000m), 20-Abril-2004, R. Novelo col. (1³, 1♀ IEXA); [Mpio. Tejupilco], Bejucos, Río Bejucos, 30-Jan-2008, R. Novelo col. (1♀ IEXA); same but 22-Abril- 2004, 700 m (15 Ƌ, 8 ♀ IEXA); [Mpio. Tejupilco], Bejucos, Río Bejucos, 08- Dic-2008, J. Curiel col. (1♀ IEXA); [Mpio. Tejupilco], Bejucos, 22 IV 2004, 1950', Río Bejucos / William D. Shepard, leg. (3³, 4♀ EMEC); Mpio. Tejupilco, Paso de Vigas, 18º 53' 32.6'' N, 100º 13' 57.0'' W, 1107 m, 19-Dec- 2012, DRV & PRH colls., L-1459 (3³, 2♀ UMC); San Felipe de Jesus, 20 IV 2004, 3280', Río San Felipe / William D. Shepard, leg. (1³ EMEC); Tejupilco, Mex., Temescaltepec [Temascaltepec], VI-18-1933 / H. E. Hinton, R. L. Usinger (3♀ EMEC); same but VI-19-1933 (9³, 3♀ EMEC); Tejupilco, Mex., Temescaltepec [Temascaltepec], VII-[no day] 1934 / H. E. Hinton (2³, 4♀ EMEC); Temescaltepec [Temascaltepec], VII-[no day] 1934 / H. E. Hinton (1♂ EMEC). MICHOACÁN: [Mpio. Aguililla], Aguililla, 03-Abril-2005, R. Novelo col. (2³, 2♀ IEXA); [Mpio. Apatzingán], Apatzingán, Pte. El Orejón, 12-Marzo-2004, R. Novelo col. (2³, 3♀ IEXA); [Mpio.] Apatzingán, California (arroyo), 21-Feb-2005, R. Novelo & J.A.Gómez cols., Ambrysus circumcinctus R. Novelo det. (1³ IEXA); Mpio. Aquila, Aquila, Pte. Ramal Aquila, 18º 35' 08.1'' N, 103º 30' 51.8'' W, 159 m, 25- Dec-2012, DRV & PRH colls., L-1487 (1³, 3♀ UMC); Mpio. Carácuaro, Carácuaro, Río San Diego, 19º 01' 04.8'' N, 101º 07' 33.2'' W, 519 m, 22-Dec-2012, DRV & PRH colls., L-1473 (2³, 1♀ UMC); [Mpio. Chinicuila], Villa Victoria, 3-Junio-2005, R. Novelo col. (1♀ IEXA); Mpio. Morelos, La Placita de Morelos, Pte. La Placita, Río Aquila, 18º 32' 19.9'' N, 103º 35' 17.9'' W, 7 m, 26-Dec-2012, DRV & PRH colls., L-1488 (6³, 1♀ UMC); Mpio. Nocupétaro, San Antonio de la Huerta, under bridge, 19º 02' 04.4'' N, 101º 15' 07.9'' W, 624 m, 22-Dec-2012, DRV & PRH colls., L-1475 (5³, 1♀ UMC); Mpio. Parácuaro, Las Yeguas, Río Tepalcatepec, 18º 53' 42.1'' N, 102º 17' 00.3'' W, 189 m, 24-Dec-2012, DRV & PRH colls., L-1480 (1³, 6♀ UMC); Mpio. Tepalcatepec, Los Horcones, km 31 carr. Tepalcatepec-Coalcomán, Pte. Pinolapa, 19º 00' 31.3'' N, 103º 01' 25.6'' W, 554 m, 25-Dec-2012, DRV & PRH colls., L-1484 (1♀ UMC); Mpio. Tepalcatepec, Río Pinolapa, 10-Jul-2006, R. Novelo, col. (1³, 2♀ IEXA); Mpio. Tepalcatepec, Tepalcatepec, 19º 11' 47.3'' N, 102º 50' 44.9'' W, 371 m, 24-Dec-2012, DRV & PRH colls., L- 1483 (1³ UMC); Mpio. Tiquicheo, Piedra China, Pte. Tiquicheo, Río Purengueo, 18º 53' 37.8'' N, 100º 45' 49'' W, 363 m, 22-Dec-2012, DRV & PRH colls., L-1472 (6³, 6♀ UMC); Mpio. Tuzantla, Tuzantla, Río Tuzantla, 19º 13' 18.1'' N, 100º 34' 05.5'' W, 593 m, 21-Dec-2012, DRV & PRH colls., L-1470 (4³, 6♀ UMC); Río Pinolapa, 30- May-2005, (4³ IEXA); S de Coalcomán, Aguililla, 11-Julio-2005, R. Novelo col. (4³, 9♀ IEXA). MORELOS: [Mpio. Amacuzac], Huajintlán, Río Amacuzac, 28 Mayo 1983, [no collector] (1♀ CNIN); same but D. Porras, L. Castrejón [colls.] (2³ CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (1³ CNIN); same locality information but / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1³, 2♀ CNIN); [Mpio. Jojutla], San Rafael Vicente Aranda, 15-Mar-1983, R. Novelo col. (3³, 4♀ IEXA); [Mpio. Jojutla], S. Rafael A., 13-VII-82, M. Espinosa, (10³, 7♀ CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (3³, 1♀ CNIN); same locality information but / A. circumcinctus Mont. det. R. W. Sites 2009 (1♀ CNIN); same locality information but / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1³ CNIN); [Mpio. Jojutla], S. Rafael A., 17-VI- 82, M. Espinosa (15³, 11♀ CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (1³, 4♀ CNIN); Mpio. Jojutla, Tehuixtla, Río Amacuzac, 18º 32' 47.9'' N, 99º 16' 03.6'' W, 854 m, 28-Apr-2011, DRV coll., L-1309 (11³, 7♀ UMC); Mpio. Jojutla, Tehuixtla, Río Amacuzac, 18º 33' 45.2'' N, 99º 16' 51.3'' W, 853 m, 28-Apr-2011, DRV coll., L-1308 (3³, 3♀ UMC); [Mpio.] Jojutla, Vicente Aranda, Río Amacuzac (900m), 11-Diciembre-1982, P. Liljehult col. (1³, 1♀ IEXA); [Mpio.] Jojutla, Vicente Aranda, Río Amacuzac (900m), 12-Feb-1983, V. García col. (27³, 40♀ IEXA); same locality information but C. Valencia col. (22³, 17♀ IEXA); [Mpio.] Jojutla, Vicente Aranda, Río Amacuzac, 27-Marzo-1983, G. Cardoso col. (12³, 14 ♀ IEXA); [Mpio.] Jojutla, Vicente Aranda, Río Amacuzac, 7-Mayo-1983, S. Ibáñez col. (7³, 7♀ IEXA); [Mpio. Jojutla], Vicente Aranda (900msnm), 16-Marzo- 2001, R. Novelo col. (4³, 7♀ IEXA); [Mpio. Jojutla], Vicente Aranda, Río Amacuzac, 04-Abril-2002, R. Novelo col. (4³, 10 ♀ IEXA); [Mpio. Tetecala], Tetecala, (900m), 02-Diciembre-2000, R. Novelo col. (1♀ IEXA); [Mpio.] Tlaquiltenango, El Astillero [La Mezquitera], Río Cuautla (dentro del río c/corriente), 5-Feb-1983, I. Oliva col. (4³, 4♀ IEXA); [Mpio. Yautepec], Yautepec, Río Yautepec (1,203m), 17-Enero-1983, I. Oliva col. (2♀ IEXA). NAYARIT: Las Adjuntas, Río Huaynamota, II 3-VII-92, S. Santiago, R. Barba (1♀ CNIN); Los Sabinos, Río Huaynamota, 10-IV-91 R. Barba, E. Barrera (1³ CNIN); same but 23-5-1991 (4³, 1♀ CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (1♀ CNIN); Mpio. Acaponeta, El Recodo, Río Acaponeta, 22° 31' 31.9'' N, 105° 20' 37.3'' W, 34 m, 01-Jul-2013, DRV & PRH colls., L-1601 (1♀ UMC); Mpio. Acaponeta, La Guásima, Río Acaponeta, 22° 24' 26.6'' N, 105° 23' 48.9'' W, 26 m, 02-Jul-2013, DRV & PRH colls., L-1602 (20♂, 17♀ UMC); Mpio. Ahuacatlán, Ejido Tetitlán, Río Tetitlán, 21° 07' 51.3'' N, 104° 37' 10.1'' W, 747 m, 02-Jul-2013, DRV & PRH colls., L-1606 (10³, 11 ♀ UMC); Mpio. Ahuacatlán, Puerta del Río, Balneario El Paraiso, Río Tetitlán, 21° 10' 59.3'' N, 104° 38' 32.9'' W, 859 m, 18-Dec-2013, DRV & PRH colls., L-1651 (1³, 1♀ UMC); Mpio. El Nayar, Playa Golondrinas, Río Santiago, 26/5/1991, R. Barba, E. Barrera / A. c. circumcinctus Montandon det. R. W. Sites (1³ CNIN); Mpio. Ruíz, San Lorenzo, Río San Pedro, 21° 57' 04.1'' N, 105° 04' 28.3'' W, 23 m, 20-Dec-2013, DRV & PRH colls., L-1662 (1³, 4♀ UMC); Mpio. San Blas, El Palillo, Pte. Palillo, Arroyo Palillo, 21° 38' 27.1'' N, 105° 08' 30.8'' W, 43 m, 20-Dec-2013, DRV & PRH colls., L-1661 (4³, 4♀ UMC); Mpio. Tepic, Colorado de la Mora, 232 msnm, 12-IV-91, E. Barrera, J. León (1♂ CNIN). NUEVO LEÓN: Mpio. Cadereyta Jiménez, San Juan, Río San Juan, 25° 31' 55.1'' N, 99° 50' 39.1'' W, 266m, 19-May-2014, DRV & PRH colls., L-1773 (15³, 23♀ UMC); Mpio. Cd. Anáhuac, Cd. Anáhuac, Río Salado, 27° 14' 09.0'' N, 100° 08' 24.1'' W, 187m, 18-May-2014, DRV & PRH colls., L-1769 (6³, 11 ♀ UMC); Mpio. García, Río Pesquerías, 8-XI-85, H. Rivas (1³ CNIN); same locality information but R. Barba / A. c. circumcinctus Montandon det. R. W. Sites (1♀ CNIN); same locality information but S. Tufinio / A. c. circumcinctus Montandon det. R. W. Sites (1♀ CNIN); Mpio. General Terán, La Barreta, Río Conchos, 25° 04' 25.9'' N, 98° 58' 55.9'' W, 124m, 20-May-2014, DRV & PRH colls., L-1776 (8³, 8♀ UMC); Mpio. General Terán, La Unión, Río El Pilón, 25° 20' 27.2'' N, 99° 34' 57.5'' W, 243m, 19-May-2014, DRV & PRH colls., L-1775 (1♀ UMC); Mpio. Lampazos de Naranjo, El Ancón, Río Candela, 27° 02' 33.5'' N, 100° 32' 13.5'' W, 293m, 18-May-2014, DRV & PRH colls., L-1770 (1³ UMC); Mpio. Los Ramones, 1 km N of Hacienda El Carrizo, Río San Juan, 25° 31' 40.5'' N, 99° 37' 20.9'' W, 206m, 19-May- 2014, DRV & PRH colls., L-1774 (24³, 23♀ UMC); [Mpio. Villaldama], Villa Aldama, [Villaldama], [Arroyo] La Coyota, 13-XI-85, R. Barba (3♀ CNIN); Pilon R. at unnamed road, across from General Terán (town), off St. Hwy 35 / 15 May 1995, DB 95-10, colls: D. E. Baumgardner & B. C. Henry (2³, 3♀ UMC); 17.5 mi. W Linares, V-25- 1981, J. T. Doyen, J. K. Liebherr (1♀ EMEC). OAXACA: Dominguillo, 7-XI-1988, E. Barrera, R. Barba, L.

Cervantes (1³ CNIN); Mpio. Ayotzintepec, San José Mano Márques, 17º 47' 26.2'' N, 96º 00' 15.3'' W, 66 m, 24- May-2012, DRV & PRH colls., L-1371 (1³, 1♀ UMC); Mpio. Guelatao de Juárez, carr. Tuxtepec-Oaxaca, 6 km W of Guelatao, Pte. Xia, 17º 18' 18.3'' N 96º 31' 33.8'' W, 1497 m, 24-May-2012, DRV & PRH colls., L-1373 (11♂, 1♀ UMC); [Mpio. Ixtepec], Río Perros, just above Ixtepec, III-27-1957, Elev. 340 ft, R. R. Miller (7³, 15 ♀ UMMZ); Mpio. Jalapa de Díaz, Sto. Domingo, Río Sto Domingo, 18° 02' 15.9'' N, 96° 32' 57.2'' W, 100 M, 25- Mar-2015, DRV, Sites, Shepard, Barr, & PRH colls., L-1881 (2³ UMC); [Mpio. San Juan Bautista Cuicatlán], Santiago Dominguillo, Río Las Huertas, 12-VII-1996, R. Barba, A. Rojas / A. c. circumcinctus Montandon det. R. W. Sites (2♀ CNIN); Mpio. San Juan Bautista Cuicatlán, Santiago Dominguillo, 17º 38' 46'' N, 96º 54' 36.7'' W, 726 m, 01-Jun-2012, DRV & PRH colls., L-1405 (4³, 2♀ UMC); Mpio. San Juan Cotzocón, Arroyo Carrizal, km 110 carr. Palomares-Tuxtepec, 17º 26' 55.6'' N, 95º 26' 39.1'' W, 94 m, 22-May-2012, DRV & PRH colls., L-1365 (3³, 7♀ UMC); Mpio. San Miguel del Puerto, Copalita, Pte. Copalita, Río Copalita, 15° 49' 12.2'' N, 96° 03' 57.6'' W, 28m, 04-Jun-2014, DRV & PRH colls., L-1818 (3³, 4♀ UMC); Mpio. San Sebastian Tecomaxthahuaca, Tecomaxthahuaca, Laguna Encantadac [in lateral channel], 17º 21' 57.5'' N, 98º 01' 13.3'' W, 1662 m, 30-May- 2012, DRV & PRH colls., L-1399 (1³, 2♀ UMC); Mpio. Santiago Jocotepec, nr. San José Río Manso, 17º 38' 44.3'' N, 95º 52' 02.1'' W, 61 m, 23-May-2012, DRV & PRH colls., L-1368 (5³, 2♀ UMC); Mpio. Santiago Jocotepec, San Jose Río Manso, Centro Ecoturístico Cerro Chango, 17º 42' 06.6'' N, 95º 54' 01.6'' W, 54 m, 23- May-2012, DRV & PRH colls., L-1367 (12³, 3♀ UMC); Mpio. Santo Domingo Zanatepec, carr. Arriaga-Santo Domingo Tehuantepec, Colonia Río Ostula, Pte. Ostula, 16º 29' 59.5'' N, 94º 26' 00.5'' W, 40 m, 22-May-2012, DRV & PRH colls., L-1360 (1³, 4♀ UMC); Mpio. Valerio Trujano, km 124 carr. Tehuacán-San Francisco Telixtlahuaca, jct to Tomellín, Pte. El Grande, Río Grande, 17º 45' 25'' N, 96º 56' 59.1'' W, 607 m, 01-Jun-2012, DRV & PRH colls., L-1406 (4³, 3♀ UMC); Mpio. Valle Nacional, San Mateo Yetla, 12-X-1990, E. Ramírez, E. Barrera, A. Cadena (1♀ CNIN); km 21 desviación San Miguel Chimalapa, 210msnm, 22-III-1990, E. Barrera, A. Cadena (1♀ CNIN); km 116 carr. Tuxtepec-Palomares, 22-XI-1990, A. Cadena, E. Barrera (1♀ CNIN). PUEBLA: [Mpio. Jopala], Patla, 23-VII-75, H. Brailovsky A. col., noct. (1³, 1♀ CNIN); same but / Ambrysus circumcinctus Montandon (Ira La Rivers) (1♀ CNIN); Mpio. Tlacuilotepec, carr. Plan de Ayala-Tlaxco, 2 km E of Papaloctipan, 20º 25' 22.4'' N, 97º 56' 38.2'' W, 310 m, 05-Jun-2012, DRV & PRH colls., L-1417 (10³, 8♀ UMC); Mpio. Tlacuilotepec, Río San Marcos, km 1 carr. A. Camacho-Petlacotla, 24-Julio-1987, R. Novelo col. (5³, 4♀ IEXA); Mpio. Tlaxco, carr. Tlaxco-Cuaxtla, ca. 2 km S of Acalman, Río Los Cajones, 20º 24' 26.8'' N, 98º 03' 51.5'' W, 785 m, 05-Jun-2012, DRV & PRH colls., L- 1418 (1♀ UMC); Mpio. Xicotepec, carr. Huauchinango-Poza Rica, Miguel Ávila Camacho (La Ceiba), Pte. San Marcos, Río San Marcos, 20º 23' 40'' N, 97º 52' 39.1'' W, 239 m, 05-Jun-2012, DRV & PRH colls., L-1416 (4³, 6♀ UMC); [Mpio. Xicotepec], Villa Ávila Camacho, [La Ceiba], Río San Marcos, 17-Julio-1990, R. Novelo col. (1♀ IEXA); same but 30-Abril-1991 (1³, 1♀ IEXA). SAN LUIS POTOSÍ: Mpio. Axtla de Terrazas, Cómoca, Río Tancuilín, 21º 25' 03.8'' N, 98º 53' 43.3'' W, 75m, 22-Apr-2011, DRV coll., L-1307 (20³, 13♀ UMC); same but 19-Jul-2011, L-1311 (1♀ UMC); Mpio. Axtla de Terrazas, Cómoca, Río Tancuilín, 21° 25' 02.9'' N, 98° 53' 42.8'' W, 86 m, 08-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1532 (1³, 5♀ UMC); Mpio. Huehuetlán, El Nacimiento de Huichihuayán (spring), 21° 27' 35.3'' N, 98° 58' 38.8'' W, 96 m, 08-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1533 (1³, 1♀ UMC); Mpio. Matlapa, Los Manantiales, Río Tancuilín, 21° 20' 45.5'' N, 98° 51' 45.8'' W, 113 m, 07-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1530 (24³, 21♀ UMC); Mpio. San Vicente Tancuayalab, Ejido El Álamo, Río Moctezuma, 21° 43' 10.9'' N, 98° 33' 16.7'' W, 32 m, 10-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1540 (17³, 16♀ UMC); Mpio. Tamazunchale, Vega Larga, 21º 15' 27.3'' N, 98º 50' 36.8'' W, 161m, 17-Jul-2011, DRV coll., L-1310 (1♂, 1♀ UMC); Mpio. Tamazunchale, Tamazunchale, Río Moctezuma, 21° 15' 27.6'' N, 98° 48' 20.6'' W, 128 m, 07-Jun- 2013, DRV, Sites, Shepard, & PRH colls., L-1529 (3³, 2♀ UMC); Mpio. Tamazunchale, Zacatipan, Río Amajac, ca. 2 km SE of Tamazunchale, 21° 14' 38.1'' N, 98° 46' 19.7'' W, 111 m, 07-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1528 (2³, 1♀ UMC); Mpio. Xilitla, ca. 1 km E of Xilitla, unnamed creek from Las Pozas, 21° 23' 30.6'' N, 98° 59' 23.3'' W, 534 m, 08-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1531 (1³ UMC); [Mpio. Xilitla], Xilitla, 600 msnm, 24-Jul-1998, R. Novelo col. (1³ IEXA); Puente Palitla [Palictla], 28-Junio-1990, R. Novelo col. (2³, 1♀ IEXA); 34 Km antes Tamazunchale, 20-V-78, J. Bueno / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1♂, 1♀ CNIN). SINALOA: Mpio. Concordia, Chupaderos, Río Pánuco, 23° 21' 49.2'' N, 105° 57' 10.3'' W, 244 m, 30-Jun-2013, DRV & PRH colls., L-1597 (2³, 2♀ UMC); [Mpio. Concordia], Río Pánuco, 38 mi E Mazatlan, Sin. Mex. VIII-28-52, J. D. Lattin / Ira La Rivers collection bequeathed to the California Academy of Sciences - 1978 (1³, CAS); [Mpio. Culiacán], Los Mayos, 24 July 1952, John D. Lattin, Cal. Acad. Sci. Coll. / Ira La Rivers collection bequeathed to the California Academy of Sciences - 1978 (2³, CAS); [Mpio. El Fuerte], El Fuerte, Río Fuerte, 26º 24' N, 108º 37' W, April 2002, W. Trimble (2³, 1♀ UMC); Mpio. Elota, Pte. Los Barriles, Arroyo El Japuino, 24° 03' 36.7'' N, 106° 48' 03.6'' W, 83 m, 22-Dec-2013, DRV & PRH colls., L-1670 (27³, 29♀ UMC); Mpio. Elota, Pte. Río Elota, Río Elota, 23° 57' 20.7'' N, 106° 42' 53.4'' W, 46 m, 22-Dec-2013, DRV & PRH colls., L-1669 (27³, 28♀ UMC); [Mpio. Mazatlán], Mazatlán, Mex., V-25-26-1934 / H. E. Hinton (1♀ EMEC); same but / Ambrysus pygmaeus La Rivers PARATYPE (1♀ EMEC); Mpio. Mazatlán, Siqueiros, Siqueiros (Dam), Río Presidio, 23° 20' 47.0'' N, 106° 14' 35.3'' W, 42 m, 21-Dec-2013, DRV & PRH colls., L-1664 (4³, 4♀ UMC); Mpio. Mazatlán, Villa Unión, Pte. Presidio, Río Presidio, 23° 11' 32.3'' N, 106° 13' 26.3'' W, 10 m, 01-Jul-2013, DRV & PRH colls., L-1598 (10³, 10 ♀ UMC); Mpio. Mocorito, Mocorito, Río Evora, 25° 28' 55.1'' N, 107° 54' 37.6'' W, 75 m, 23-Dec-2013, DRV & PRH colls., L-1674 (12³, 13 ♀ UMC); Mpio. Rosario, Matatán, 16 km W of El Rosario, Río Los Cedros, 23° 01' 54.1'' N, 105° 43' 52.0'' W, 55 m, 21-Dec-2013, DRV & PRH colls., L-1663 (3³ UMC); Mpio. San Ignacio, San Ignacio, Río Piaxtla, 23° 56' 06.3'' N, 106° 25' 41.0'' W, 127 m, 21-Dec-2013, DRV & PRH colls., L-1666 (1³ UMC); Mpio. Sinaloa de Leyva, Burague, Río Sinaloa, 25° 49' 25.4'' N, 107° 57' 49.4'' W, 140 m, 24-Dec-2013, DRV & PRH colls., L-1678 (34♂, 23♀ UMC). SONORA: [Mpio. Álamos], Arroyo Cuchujaqui, 7 mi. SE Álamos, Son., Mex., VI-19-63 / J. Doyen Collector (1♀ EMEC); Mpio. Álamos, El Naranjo, Arroyo El Taymuco, 27° 14' 43.3'' N, 108° 45' 27.8'' W, 462 m, 25-Dec-2013, DRV & PRH colls., L-1682 (5³, 3♀ UMC); Mpio. Álamos, Taymuco (El Vado), Arroyo La Vinatería, 27° 14' 39.7'' N, 108° 43' 17.3'' W, 537 m, 25-Dec-2013, DRV & PRH colls., L-1683 (1³, 2♀ UMC); [Mpio. Álamos], 8 mi S Álamos, 12 Sept 79 / Arroyo Cucyacho in River, C. Y. Kitayama (1³, 3♀ EMEC); Mpio. Onavas, Tepoca, Arroyo Tepoca, 28° 25' 50.3'' N, 109° 15' 26.0'' W, 564 m, 27-Dec-2013, DRV & PRH colls., L-1691 (25³, 9♀ UMC); Mpio. Quiriego, Tepahui, 27° 23' 27.3'' N, 109° 11' 03.6'' W, 137 m, 26-Dec-2013, DRV & PRH colls., L-1687 (1♀ UMC); Mpio. Rosario de Tesopaco, Nuri, Arroyo Nuri, 28° 06' 39.0'' N, 109° 19' 15.5'' W, 357 m, 27-Dec-2013, DRV & PRH colls., L-1688 (7³, 15 ♀ UMC); Mpio. Rosario de Tesopaco, ca. 6 km N of Nuri, Arroyo Cajón de Amador, 28° 09' 50.0'' N, 109° 19' 37.7'' W, 324 m, 27-Dec-2013, DRV & PRH colls., L-1689 (11³, 13 ♀ UMC); Mpio. Soyopa, ca. 15 km E of Barranca, Río Yaqui, 28° 34' 21.2'' N, 109° 33' 11.6'' W, 173 m, 27-Dec-2013, DRV & PRH colls., L- 1690 (1♀ UMC); 10 miles so[uth] Alamos, VI 13 1961 / F. D. Parker (1³, 2♀ UCDC). TABASCO: Mpio. Tlacotalpa, [Tapijulapa], Río de la Sierra, 29/III/1998, 17º 28' 04'' N, 92º 46' 52'' W, J. Bueno. R. Barba (2♂, 2♀ CNIN). TAMAULIPAS: Mpio. Aldama, Las Nubes, Pte. San Rafael, 23° 09' 56.4'' N, 97° 57' 14.2'' W, 37m, 23- May-2014, DRV & PRH colls., L-1789 (1³, 1♀ UMC); Mpio. Güemes, ca. 6 km W of Estación Santa Engracia, Río Corona, 24° 00' 31.4'' N, 99° 10' 04.1'' W, 216m, 21-May-2014, DRV & PRH colls., L-1782 (5³, 11 ♀ UMC); Mpio. Güemes, La Gloria, Río Corona, 24° 00' 30.5'' N, 99° 09' 24.0'' W, 214m, 21-May-2014, DRV & PRH colls., L-1781 (17³, 6♀ UMC); Mpio. Juamave [Jaumave], Los Nogales, 2-xi-99, R. Jones, matorral (1♀ UAQE); Mpio. Padilla, El Barretal, Pte. Purificación Poniente, Río Purificación, 24° 04' 43.4'' N, 99° 07' 23.9'' W, 194m, 21-May- 2014, DRV & PRH colls., L-1779 (12³, 7♀ UMC); Mpio. Padilla, El Barretal, Río Purificación, 24° 04' 40.5'' N, 99° 08' 31.3'' W, 205m, 21-May-2014, DRV & PRH colls., L-1780 (9³, 7♀ UMC); Mpio. San Fernando, Las Norias, Arroyo Chorreras, 24° 36' 30.0'' N, 98° 17' 54.6'' W, 70m, 21-May-2014, DRV & PRH colls., L-1777 (36♂, 40♀ UMC); 15 km N of C. Victoria, 18 March 1983, coll. M. S. Davis (1³, UMC). VERACRUZ: La Palma, 6/V/ 81, G. Ortega L. (1³, CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (1♀ CNIN); Las Palmas, 13-V-83, G. Ortega / Ambrysus hybridus det. M. G. Ortega / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1³, 1♀ CNIN); Las Palmas, 13-V-83, G. Ortega / Ambrysus hybridus det. M. G. Ortega / A. c. circumcinctus Montandon det. R. W. Sites (1³ CNIN); same but 18-V-83 (1³ CNIN); Los Tuxtlas, La Palma, 13- V-83 G. Ortega L. / Ambrysus hybridus M. G. Ortega / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1♀ CNIN); [Mpio. Atzalán], Tomata, Pte. Tomata, Río Alseseca, 06-Abril-2011, V. Amaya col. (1♀ IEXA); Mpio. Atzalán, Pte. Tomata, Río Alseseca, 19º 55.335' N, 97º 13.385' W, 500 m, 6-Apr-2011, Sites, Shepard, Amaya, Novelo colls., gravel riffles, L-1296 (2♀ UMC): Mpio. Atzalán, Pte. Tomata, Río Alseseca, 19° 55' 15.7'' N, 97° 13' 28.2'' W, 514 m, 13-Jun-2013, DRV, Sites, Shepard, Novelo & PRH colls., L-1549 (6♂, 2♀ UMC); [Mpio. Atzalán], W of Tlapacoyan, El Filo, Río Filobobos, 08-Noviembre-2009, R. Novelo col., Ambrysus circumcinctus R. Novelo det. (33³, 50♀ IEXA); Mpio. Catemaco, Arroyo a 3 km Margaritas, 11-V-2012, J. L. Villalobos (3³, 1♀ CNIN); [Mpio. Chicontepec], Chicontepec, 14-IV-75, H. Brailovsky / Ambrysus circumcinctus Montandon (Ira La Rivers) (1³ CNIN); Mpio. Citlaltepec, Citlaltepec, 21°19' 47.7'' N, 97° 53' 00.1'' W, 194 m, 11- Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1544 (2³, 1♀ UMC); Mpio. Coatepec, Agua Alegre, Río Huehueyapan, 18 [19º] 26.884' N, 96 58.867' W, 1198 m, 4 IV 2011 / William D. Shepard leg., L-1290 (2³ UMC); [Mpio. Coatepec], Coatepec, Río Huehueyapan, 04-Abr-2011, R. Novelo Col. (1♀ IEXA); [Mpio. Coscomatepec], Coscomatepec, 29-I-84, L. Becerra (1♀ CNIN); [Mpio. Huatusco]: Huatusco, Río Jamapa (rápidos), 16-Agosto- 1986, M. Calvillo Col. (1♀ IEXA); Mpio. Ixhuatlán de Madero, Naranjo Dulce, Río Vinazco, 20° 49' 24.9'' N, 97° 55' 19.2'' W, 86m, 27-May-2014, DRV & PRH colls., L-1798 (3♀ UMC); [Mpio. Jamapa], Jamapa, 1200 mts, 1-V- 1954, C. Bolivar (2♀ CNIN); same but / A. c. circumcinctus Montandon det. R. W. Sites (1³ CNIN); Mpio. Misantla, Libertad, Río Kilate, 20o 03' 06.2" N, 96 o 58' 39.4" W, 61 m, 14-Jun-2013, DRV, Sites, Shepard, Novelo, & PRH colls., L-1552 (47³, 47♀ UMC); Mpio. Misantla, Pte. Chapachapa, Río Chapachapa, 19o 58' 12.5" N, 96 o 53' 07.6" W, 121m, 14-Jun-2013, DRV, Sites, Shepard, Novelo, & PRH colls., L-1554 (1³, 5♀ UMC); Mpio. Paso de Ovejas, Paso de Ovejas, 19° 17' 04.1'' N, 96° 26' 03.7'' W, 40m, 29-May-2014, DRV & PRH colls., L-1802 (1♀ UMC); Mpio. Puente Nacional, Puente Nacional, Pte. Puente Nacional I, Río La Antigua, 19° 19' 28.5'' N, 96° 28' 54.3'' W, 67m, 29-May-2014, DRV & PRH colls., L-1801 (1³, 1♀ UMC); [Mpio. San Andrés Tuxtla], Est. Biol. Los Tuxtlas, 20-I-86, col. F. Arias & C. Mayorga (3³, 3♀ CNIN); [Mpio. San Andrés Tuxtla], Estación de Biología Los Tuxtlas, 28-IV-1991, G. Ortega, C. Mayorga / A. c. circumcinctus Montandon det. R. W. Sites (1♂, 1♀ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, El Salto de Eyipantla, 6-5-81, M. García, (1♀ CNIN); same locality information but R. Novelo / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1³ CNIN); same locality information but R. Arce (1³ CNIN); same but / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1♀ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, L[aguna] Escondida, 5-XII-75, J. Bueno / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] / Ambrysus circumcinctus caliginosus Usinger det. R. Sites 2009 (1♀ CNIN); [Mpio. San Andrés Tuxtla], Tuxtlas, L[aguna] Escondida, 8-XII-75, J. Bueno (1♀ CNIN); same but / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1³ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, Río La Palma, Ver., 7-V-81, R. Novelo / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1♀ CNIN); same locality information but 02-Abril-2011, R. Novelo col. (3³ IEXA); [Mpio. San Andrés Tuxtla], Los Tuxtlas, Río Máquinas, 14-[VII or VIII, unclear]-79, J. Bueno / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (2³ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, Río Máquinas, 5-V-81, R. Novelo (1³ CNIN); same locality information but 5-5-81, M. García (2♀ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, Río Máquinas, 11-V-81, Herández / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1♀ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, Río Máquinas, 12-5-83, G. Ortega / Ambrysus hybridus det. G. Ortega (1♀ CNIN); [Mpio. San Andrés Tuxtla], Los Tuxtlas, Río Máquinas, 02-Abril-2011, R. Novelo col. (2³, 2♀ IEXA); Mpio. Sta. Maria, E of Ixcatepec, 21° 17' 25.8'' N, 97° 54' 56.8'' W, 223 m, 11-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1543 (4³, 7♀ UMC); Mpio. Tantoyuca, N of Platon Sánchez, El Remanzo, Río Calabazo, 21° 17' 08.2'' N, 98° 22' 38.6'' W, 41 m, 06-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1524 (6♂, 6♀ UMC); [Mpio.] Tlapacoyan, El Encanto, Río Filobobos, 104 msnm, 8-XI-2009, 19º 59' 01'' N, 97º 10' 08'' W, L. Cervantes, R. Sites, R. Novelo (1³, 1♀ CNIN); [Mpio.] Tlapacoyan, El Encanto, Río Filobobos, 08-Nov-2009, R. Novelo col. (4³, 11 ♀ IEXA); [Mpio.] Tlapacoyan, Ixtacuaco, 30-Marzo-2003, R. Novelo col. (1♀, IEXA); Mpio. Tlapacoyan, Río Bobos, 19o 55' 39.8" N, 97 o 09' 45.5" W, 281 m, 14-Jun-2013, DRV, Sites, Shepard, Novelo, & PRH colls., L-1551 (97³, 121♀ UMC); Mpio. Tlapacoyan, Río Filobobos, El Encanto, 8 November 2009, L-1123 / 19º 59.022' N, 97º 10.149' W, 178 m, Sites, Cervantes, Novelo (1³ UMC); Mpio. Tlapacoyan, Río Filobobos, 19º 55.662' N, 97º 09.764' W, 267 m, 8 Nov 2009, Sites, Cervantes, Novelo colls., L-1124 (16³, 34♀ UMC); Mpio. Tlapacoyan, Rojo Gómez, Río Filobobos, 20° 02' 41.7'' N, 97° 05' 38.5'' W, 89 m, 12-Jun-2013, DRV, Sites, Shepard, & PRH colls., L-1548 (11³, 8♀ UMC); [Mpio.] Tlapacoyan, Ruinas El Cuajilote (Filobobos) 08-Nov- 2009, R. Novelo col. (5³, 2♀ IEXA); [Mpio. Tlapacoyan], Tlapacoyan, El Muro (arroyo) 900 msnm, 13-Apr- 2004, R. Novelo col. (1³ IEXA); [Mpio. Tlapacoyan], Tlapacoyan, Río Tomata, 25/V/1985, J. Bueno, R. Barba (2♀ CNIN); [Mpio. Tlapacoyan], Tlapacoyan, 28-Marzo-1984, H. Velasco col. (1♀ IEXA); [Mpio. Zongolica], Zongolica, 11-VIII-74, J. Bueno (1³ CNIN); same but / Ambrysus circumcinctus Montandon (Ira La Rivers) [det.] (1³ CNIN); [Mpio. Zongolica], Zongolica, 11-VIII-74, J. Bueno / H. Brailovsky A. det. Ambrysus circumcinctus Montandon (1♀ CNIN); San Andrés Tuxtla, 30-VII-76, J. Bueno / Ambrysus circumcinctus Montandon det. J.T. Polhemus 19[??] (1♂ CNIN). ZACATECAS: [Mpio. Moyahua de Estrada], Moyahua, 13 I 2005, 3610', Río Juchipila / William D. Shepard, leg. (8³, 7♀ EMEC); [Mpio. Moyahua de Estrada], Río Juchipila at Moyahua, 13 Jan. 2005, C. B. Barr / 21º 16.29' N, 103º 10.33' W, elevation ~ 3850 ft. (1³, 2♀ EMEC); [Mpio. Moyahua de Estrada], Río Juchipila, 9.1 rd. km S of Moyahua, 13 Jan. 2005, C. B. Barr / 21º 12.63' N, 103º 10.84' W, elevation ~ 3800 ft. (1³, 2♀ EMEC); [Mpio. Moyahua de Estrada], 9.1 km S Moyahua, 13 I 2005, 3610', Río Juchipila / William D. Shepard, leg. (1♂, 6♀ EMEC).

Figures

FIGURE 7. Structures of Ambrysus circumcinctus collected in southern Mexico (L- 1901). (A) dorsal habitus of male, (B) 6 th and 7 th abdominal terga of male, (C) 8 th abdominal tergum of male, (D) genital capsule of male (phallosoma removed), (E) ventral side of phallosoma, (F) 6 th abdominal sternum and subgenital plate of female (terminal segments removed). Size bar = 0.5 mm and applies only to Figs. D and E.

FIGURE 8. Distribution of Ambrysus circumcinctus in Mexico (topographic map modified from Mexico map relief by Carport and used under CC BY 3.0).