Resupinatus yunnanensis Y. Yang & C.L. Zhao, 2023

Yang, Yang, Li, Rong, Liu, Chaomao & Zhao, Changlin, 2023, Morphological and molecular identification for two new species of woodinhabiting macrofungi (Basidiomycota) from Yunnan-Guizhou Plateau, China, Phytotaxa 591 (1), pp. 1-18 : 10-12

Resupinatus yunnanensis Y. Yang & C.L. Zhao , sp. nov. Figs. 1 , 3 , 6 , 7

MycoBank no.: MB 847020

Holotype: — CHINA. Yunnan Province, Pu’er, Jingdong County, Taizhong Township , Xujiaba , Ailaoshan Ecological Station , E 100°53′41′′, N 24°23′36′′, elev. 1800 m, on the fallen branch of angiosperm, 24 August 2018, CLZhao 8651 ( SWFC!), GenBank No. (ITS OP901839; nLSU OP904197).

Etymology: — yunnanensis (Lat.) : refers to the locality (Yunnan Province) of the type specimen.

Basidiomata: —Annual, resupinate to cupulate, soft gelatinous when fresh, fragile when drying, up to 12 cm long, 3 cm wide, 0.5 mm thick, cups globose or depressed-globose, up to 150–500 μm in diameter, aggregated in groups, 3–6 per mm, buff (4A4) to slightly brown (5B/C7), covered in a dense mat of white hairs; hymenophore cyphelloid, buff (4A4) to slightly brown (5B/C7) when fresh, greyish (19C2) to pale mouse-grey (7C2) upon drying; sterile margin indistinct and slightly brown; subiculum felty, loosely attached to the substrate and densely packed, up to 200 μm thick.

Hyphal structure: — Hyphal system monomitic; generative hyphae with clamp connections, colorless, thin-walled, branched, interwoven, 1.5–3.5 μm in diam; IKI–, CB–, tissues unchanged in KOH. presence of crystal encrusted branched hyphae with short finger-like projections at the tips.

Hymenium : — Cystidia and cystidioles absent; basidia clavate, 12–36.5 × 4–8 µm, with 4-sterigmata and with a basal clamp connection, basidioles dominant, in shape similar to basidia, but slightly smaller.

Basidiospores: —Ellipsoid, colorless, thin-walled, smooth, with oil drops, IKI–, CB–, 4.5–9(–9.5) × 3.5–7 µm, L = 6.68 µm, W = 4.72 µm, Q = 1.32–1.71 (n = 90/3), Qm = 1.42.

Additional specimens examined:— CHINA. Yunnan Province, Pu’er, Jingdong County, Taizhong Town , Ailaoshan , E 100°51′30′′, N 24°25′42′′, elev. 1800 m, on the fallen branch of angiosperm, 4 October 2017, CLZhao 3802 ( SWFC!), GenBank No. (ITS OP901840 ); Chuxiong, Zixishan Forestry Park , E 101°24′06′′, N 25°03′60′′, elev. 2100 m, on the trunk of angiosperm, 1 July 2018, CLZhao 7168 ( SWFC!), GenBank No. (ITS OP901838 ).

Notes: Resupinatus alboniger (Pat.) Singer, Beih. (1977: 17) , R. cinerascens (Cleland) Grgur.(1997: 47) , R. hyalinus (Singer) Thorn, Moncalvo & Redhead (2006: 1148) and R. vinosolividus (Segedin) J.A. Cooper (2012: 1) resemble R. yunnanensis by having the elliptical basidiospores. However, Resupinatus alboniger is distinguished from R. yunnanensis by the brown hymenophore and irregular finger-like cystidia (23–35 × 8–10 µm) ( Gonou-Zagou et al. 2011); R. cinerascens differs in R. yunnanensis by having the grayish black hymenophore and diverticulate branching cystidia (19–35.5 × 2.5–6 µm) ( Mcdonald 2015); R. hyalinus is separated from R. yunnanensis by having the surface hairs and finger-like cystidia ( Mcdonald 2015); R. vinosolividus differs from R. yunnanensis by having the cheilocystidia at edge of basidiomata on top surface of gills ( Mcdonald 2015).

Resupinatus yunnanensis resembles R. applicatus (Batsch) Gray (1821: 617) , R. huia (G. Cunn.) Thorn, Moncalvo & Redhead (2006: 1148) , R. incanus (Kalchbr.) Thorn, Moncalvo & Redhead (2006: 1148) and R. poriaeformis (Pers.) Thorn, Moncalvo & Redhead (2006: 1148) in having cupulate basidiomata. However, Resupinatus applicatus is different from R. yunnanensis by having the brown to black hymenophore with a lateral pseudostipe, diverticulate with finger-like cystidia and globose to subglobose basidiospores ( Mcdonald 2015); R. huia can be delimited from R. yunnanensis by its simple septa generative hyphae and oblong basidiospores ( Thorn et al. 2005); R. incanus differs from R. yunnanensis by grey to black basidiomata and oblong basidiospores ( Thorn et al. 2005); R. poriaeformis is distinguished from R. yunnanensis by dark brown or grey basidiomata, diverticulate cystidia, and globose to subglobose basidiospores and tan to ( Thorn et al. 2005).

Figures

FIGURE 1.MaximumParsimonystrictconsensus treeillustratingthephylogeny oftwonew speciesandrelatedspecies inAgaricomycetidae based on ITS+nLSU sequences. Branches are labeled with maximum likelihood bootstrap values equal to or above 70%, parsimony bootstrap values equal to or above 50% and Bayesian posterior probabilities equal to or above 0.95. The new species are in bold.

FIGURE 3. Maximum Parsimony strict consensus tree illustrating the phylogeny of the new species of Resupinatus based on ITS sequences. Branches are labeled with maximum likelihood bootstrap values equal to or above 70%, parsimony bootstrap values equal to or above 50% and Bayesian posterior probabilities equal to or above 0.95.

FIGURE 6. Basidiomata of Resupinatus yunnanensis. Bars: A = 2 cm, B = 1 mm (holotype). Photos by: Yang Yang

FIGURE 7. Microscopic structures of Resupinatus yunnanensis (drawn from the holotype). A: Basidiospores. B: Basidia and basidioles. C: The structure of some crystal encrusted branched hyphae on the cuticle of the fruit bodies. D: Hyphae from context. E: Section of hymenophore trama. Bars: A = 20 µm, B–E = 10 µm. Drawings by: Rong Li