Chaetocladius lopatinskiy Makarchenko et Makarchenko

Makarchenko, Eugenyi A., Makarchenko, Marina A. & Semenchenko, Alexander A., 2017, New or little-known species of Chaetocladius s. str. Kieffer, 1911 (Diptera: Chironomidae: Orthocladiinae) from the Amur River basin (Russian Far East), Zootaxa 4247 (3), pp. 313-330 : 320-322

Chaetocladius lopatinskiy Makarchenko et Makarchenko , sp. nov.

( Figs. 19–28 , 40–41 )

Chaetocladius laminatus Brundin, 1947 ; Makarchenko et al. 2014: 424, misidentification.

Material. Holotype: adult male, Russian Far East, Jewish Autonomous Region, Obluchie District, Lopatinskyi Spring of Bidzhan River basin (Amur River basin), N 48°37' 810'', E 131°39' 114'', 31.III. 2015, leg. E. Makarchenko . Paratypes: 1 mature pupa of male, the same data as holotype , 31.III. 2 015; 2 males, 1 mature pupa of male, the same data as holotype except, Fedotkin Spring of Bidzhan River , N 48°38' 409'', E 131°37' 217'' , 5. IV. 2014, leg. E. Makarchenko.

Adult male (n=3). Total length 3.5–4.0 mm. Wing length 2.28–2.76 mm. Total length/wing length 1.27–1.62. Colouration brown to dark brown.

Head. Eyes bare, with dorsomedian prolongation. Temporal setae include (from one side) 6–11 verticals and 5– 10 postorbitals. Clypeus with 8–10 setae. Antenna with 13 flagellomeres and well developed plume; top of flagellomere 13 pointed; AR 2.14–2.33. Lengths of palpomeres 2–5 (in µm): 60–64: 164–182: 164–172: 244– 264. Third palpomere in distal part with 10 sensilla clavata.

Thorax. Dark brown. Antepronotum with 2–7 lateral setae. Acrostichals 16–23, beginning close to antepronotum; dorsocentrals 11–15; prealars 5–6; scutellum with 6–8 setae in 1 row.

Wing. Halteres dark brown. R with 9–13 setae, R1 with 1–2 setae, R4+5 with 0–2 setae subapically. Costa extension 60–96 µm. Apex of R4+5 distal of apex M3+4. Cu1 curved in apical quarter. Anal lobe developed, rectangular-rounded. Squama with 13–16 setae.

Legs. Spur of fore tibia 64–84 µm. Spurs of mid tibia 28–32 µm and 24–28 µm long, of hind tibia 64–72 µm and 22–28 µm long; ta1 of fore leg with 0–2 pseudospurs; ta1– ta3 of mid and hind legs 2 pseudospurs. Hind tibial comb with 15–18 setae. Pulvilli small. Length (in µm) and proportions of leg segments as in Table 3 .

continued.

Hypopygium ( Figs. 19–22 , 40 ). Tergite IX with 24–35 long setae and narrow naked anal point 28–40. Laterosternite IX with 6–8 setae on each side. Transverse sternapodeme rounded, 144–156 µm long, with oral projections like tubercles. Virga 16–32 µm long, consists of 2–3 small spines. Gonocoxite 268–304 µm long; inferior volsella as a rounded projection covered with microtrichia and setae ( Figs. 19 , 40 ). Gonostylus 11 2–120 µm long, triangular shape, with megaseta 16 µm long and strong long seta close ( Figs. 19–21 ). In some positions gonostylus might look like with projection ( Fig. 22 ).

Pupa (n=2). Total length 4.0– 4.65 mm.

Cephalothorax. Frontal apotome with 2 setae 56–88 µm long. Antepronotum with 2 median and 1 lateral antepronotals 80–96 µm long. Thorax yellowish-brown and smooth. Thoracic horn 232–280 µm long, with pointed apex and covered with small spinules in distal 2/3, but sometimes spinules only in subapical part ( Figs. 23–25 ). Precorneal setae hair-like, 64–112 µm long. Dorsocentrals hair-like, 44–120 µm long. Distance between Dc1 and Dc2 96–136 µm; between Dc2 and Dc 3 28–32 µm; between Dc3 and Dc 4 20–30 µm.

Abdomen. Tergite I with shagreen of not numerous spinules. Tergites II–VIII uniformly covered with shagreen spinules of one size and with large spines (20–44 µm long) along posterior edge ( Figs 26–28 ); anal lobes with not numerous spinules in proximal part ( Figs. 27–28 ). Sternites I–II without shagreen of spinules; sternite III with group of 7–16 spinules in middle part near posterior edge; sternites IV–VIII with shagreen of spinules as in tergites but spines near posterior edge not so large. Segment I with 0–1 pair of hair-like lateral setae. Segments II–VII with 2 pairs of seta-like lateral setae on tubercles and 2 pairs of hair-like setae. Length of lateral setae of segment IV: LS1 (seta-like on tubercle) 116 µm; LS2 (hair-like) 136 µm; LS3 (seta-like on tubercle) 116 µm; LS4 (hair-like) 80 µm. Segment VIII with 3–4 pairs of hair-like lateral setae 60–88 µm long. Anal lobe 280–320 µm long, with 3 spinelike macrosetae; anterior macroseta (AM1)104–136 µm long and 8 µm wide; middle macroseta (AM2) 148–168 µm long and 8 µm wide; posterior macroseta (AM3) 112–164 µm long and 8 µm wide. Distance from base of anal lobe to AM1 0.44–0.52 of anal lobe length. Distance between AM1 and AM2 64–80 µm; between AM2 and AM 3 24–32 µm. Location of anal megasetae illustrated in Figs. 27–28 , 41 . Male genital sac extending 40 µm beyond anal lobe.

Diagnostic characters. This new species is closely related to C. laminatus Brundin , but the pupa of C. lopatinskiy sp. nov. has a longer body (3.95–4.1 mm vs. 3.0– 3.5 mm), thoracic horn (232–280 µm vs 120–138 µm)

Figures

FIGURES 19 – 28. Chaetocladius lopatinskiy sp. nov., adult male (19 – 22) and pupa (23 – 28). 19, hypopygium in dorsal view; 20 – 22, gonostylus in varies positions; 23 – 25, thoracic horn; 2 6, tergites VI – VII; 27, tergite VIII and anal segment of female; 28, the same of male. Scale bars—Figs. 19 – 25 — 50 µm; Figs. 26 – 28 — 200 µm.

FIGURES 40 – 44. Chaetocladius lopatinskiy sp. nov. (40 – 41) and Chaetocladius yavorskayae sp. nov. (42 – 44), adult male (40, 42) and pupa (41, 43 – 44). 40, 42, hypopygium in dorsal view; 41, 43, anal segment of male; 44, spinules of tergite IV.

Tables

TABLE 3. Lengths (in µm) and proportions of leg segments of Chaetocladius lopatinskiy sp. nov., male (n = 3).

	fe	ti	ta1	ta2	ta3	ta4	ta5
P1	912–1024	1040–1200	704–800	400–480	320–352	192–240	144–160
P2	944–1088	1008–1136	464–512	280–320	224–256	144–176	128–144
P3	1008–1216	1184–1360	688–768	400–448	304–352	192–208	144–160