Aspergillus sibiricus V.A. Iliushin,

Iliushin, Vadim A., 2022, Aspergillus sibiricus (Aspergillaceae, Eurotiales), a novel acid-tolerant species in Aspergillus section Fumigati, Phytotaxa 531 (1), pp. 63-72 : 68-69

Aspergillus sibiricus V.A. Iliushin, sp. nov. ( Fig. 2 )

MB 841752

Type:— RUSSIA. Siberia: Altai Republic, Kosh-Agach area, Chagan-Uzun village (50°10’24’’N, 88°36’15’’E): from acid (pH 3.2) soil open coal mine, 2015, isol. V . A. Iliushin , (holotype LE F-341005 dried culture); ex-type culture CBS 143307 . ITS barcode: MG 587008 (alternative markers: BenA = MG 722970 ; CaM = MG 722971 ; RPB2 = MG 710809 ) .

Etymology:— Latin “sibiricus” refers to the place of collection (Siberia).

Diagnosis:— A. sibiricus has subglobose to globose vesicles, ampulliform phialides and produces globose, smooth conidia. It is able to grow at 47 °C but does not grow at 50 °C.

Description:— Colony diam. at 7 days (mm). 25 °C: CYA 28–32, MEA 27–32, CZ 25–30, YES 40–44, OA 26–33, M20S 35–40. 30 °C: CYA 35–40, MEA 35–40. 37 °C: CYA 47–51, MEA 40–45.

Colony characters:— Colonies on CYA, 7 days, 25 °C are pale greenish yellow (#ebe8a4), velvety; conidiogenesis abundant; sclerotia absent; reverse uncoloured. Colony characters on CYA, 30 °C and on CYA, 37 °C are the same as characters on CYA, 7 days, 25°C. Colonies on MEA, 7 days, 25 °C are greenish white (#dfede8) or almost white (#f2f3f4), plane, velvety to powdery, conidiogenesis few in number; reverse vivid yellow (#F3C300); sclerotia absent. Colony characters on MEA, 30 °C and on MEA, 37 °C are the same as characters on MEA, 7 days, 25°C. Colonies on CZ, 7 days, 25 °C are pale greenish yellow (#ebe8a4), plane; conidiogenesis abundant; sclerotia absent; reverse uncoloured. Colonies on YES, 7 days, 25 °C are grayish white (#dfede8), almost plane, slightly convex in the centre, velvety-felt; conidiogenesis abundant; sclerotia absent; reverse uncoloured. Colonies on OA, 7 days, 25 °C are almost white (#f2f3f4), plane, powdery; conidiogenesis abundant; sclerotia absent; reverse uncoloured. Colonies on M20S, 7 days, 25 °C are white (#f2f3f4), plane, slightly velvety, conidiogenesis few in number; reverse vivid yellow (#F3C300); sclerotia absent ( Fig. 2 ).

Micromorphology:— mycelium branched, septate, smooth-walled hyphae, 2.0–6.0 μm wide. Conidial heads columnar, uniseriate. Conidiophores 50–250 μm long, 2.5–5.0 μm wide in the middle, smooth-walled, non-septate or with occasional septum, lower part occasionally greenish yellow. Vesicles subglobose to globose, 8–16 μm in diameter. Aspergilla uniseriate; phialides ampulliform, 3–5 × 1.5–2.5 μm, covering two-thirds of the vesicle. Conidia uncoloured in mass, globose 1.5–2.2 μm, smooth ( Fig. 2 ).

Notes:— A. sibiricus is able to grow at 47 °C and does not grow at 50 °C. A. sibiricus grows in a wide pH range of 2–10 with an optimum of 4–7.

Figures

FIGURE 2. Morphological characters of Aspergillus sibiricus. a. CYA. b. MEA.c. OA. d. M20S. e. CYA reverse. f. MEA reverse. g. YES. h. CZ. i, j. Conidiophores. k. Conidia. Scale bar = 10 μm.