Cadophora novi-eboraci Travadon, D.P.Lawr., Roon.-Lath., Gubler, W.F.Wilcox, Rolsh. & K.Baumgartner

Bien, Steffen & Damm, Ulrike, 2020, Arboricolonus simplex gen. et sp. nov. and novelties in Cadophora, Minutiella and Proliferodiscus from Prunus wood in Germany, MycoKeys 63, pp. 119-161 : 119

Cadophora novi-eboraci Travadon, D.P.Lawr., Roon.-Lath., Gubler, W.F.Wilcox, Rolsh. & K.Baumgartner Figures 5E , 10

Description.

Sexual morph not observed. Asexual morph on SNA. Vegetative mycelium hyaline, smooth-walled, septate, branched, 1-4 µm wide, sometimes hyphae inflated and constricted at the septa, chlamydospores absent. Sporulation abundant, conidia formed on hyphal cells. Conidiophores hyaline, smooth-walled, mostly simple, rarely septate and branched, up to 20 µm. Conidiogenous cells enteroblastic, hyaline, smooth-walled, often integrated, discrete conidiogenous cells ampulliform, ellongate-ampulliform to navicular, 7-17 × 1.5-3 µm, necks cylindrical, 1-1.5 × 1.5-5.5 µm, collarettes cylindrical to narrowly funnel-shaped, 1.5-2 µm long, 0.5-1.5 µm wide at the upper edge, opening 0.5-1 µm, periclinal thickening sometimes observed. Conidia aggregated in heads, hyaline, smooth-walled, aseptate, cylindrical, elongate-ellipsoidal to ellipsoidal, straight, rarely slightly curved, with both ends rounded, (3-)4.5-6.5(-8.5) × 1.5-2(-2.5) µm, mean ± SD = 5.4 ± 1.1 × 1.8 ± 0.4 µm, L/W ratio = 2.9.

Culture characteristics.

Colonies on SNA flat with an entire to undulate margin, hyaline to pale smoke grey, filter paper partly pale luteous to very pale smoke grey, lacking aerial mycelium, reverse same colours, 5-7 mm diam. in 2 wk (25 °C in the dark); Colonies on OA flat with an entire to undulate margin, fawn to umber with a pale luteous to luteous margin, partly covered by floccose white aerial mycelium, reverse fawn, pale olivaceous to pale luteous, 18 mm diam. in 2 wk (25 °C in the dark).

Notes.

In total, eight strains of C. novi-eboraci were isolated from Prunus cerasus in Saxony (7) and Bavaria (1). Five of the strains from Saxony and the strain from Bavaria had been selected for the phylogenetic analyses. The complete sequence dataset of C. novi-eboraci exhibits a certain amount of variation in the loci analysed. The ITS and EF-1α sequences exhibited a maximum of one and two nucleotide differences to those of the ex-type strain NYC14, respectively. The TUB sequences were more variable; the TUB sequence of strain NYC13 differs in 15 nucleotides from that of NYC14. The TUB sequences of the strains from this study only differ with a maximum of two nucleotides from the ex-type strain.

Material examined.

Germany, Bavaria, in garden east of Wolferszell, 48°57'38.8"N, 12°38'24.9"E, from non-symptomatic wood of Prunus cerasus , 2 Oct 2016, J. Simmel leg., GLM-F110552, culture GLMC 1472 = CBS 145758 = DSM 109145.

Figures

Figure 5. Colony surface of analysed strains on OA medium. A Arboricolonus simplex GLMC 459 T B Cadophora africana CBS 120890 T C C. bubakii CBS 198.30 T D C. luteo-olivacea GLMC 1264 E C. novi-eboraci GLMC 1472 F C. obscura CBS 269.33 G C. prunicola CBS 120891 T H C. prunicola GLMC 1633 I C. ramosa GLMC 377 T J Minutiella pruni-avium GLMC 1624 T K Proliferodiscus ingens GLMC 1751 T L Proliferodiscus sp. GLMC 460. Cultures A, J-L after 4 wk. Cultures B-I after 2 wk. Strains with a superscript T are ex-type cultures.

Figure 10. Cadophora novi-eboraci A-G conidiophores and conidiogenous H conidia A-H from SNA A-H LM. Scale bar: 5 μm (A applies to B-H).