Neoascochyta yunnanensis Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv, 2024

Wang, Yuchun, Tu, Yiyi, Chen, Xueling, Jiang, Hong, Ren, Hengze, Lu, Qinhua, Wei, Chaoling & Lv, Wuyun, 2024, Didymellaceae species associated with tea plant (Camellia sinensis) in China, MycoKeys 105, pp. 217-251 : 217-251

Neoascochyta yunnanensis Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv sp. nov.

Fig. 9

Etymology.

Named after the location where it was collected, Yunnan Province.

Description.

Sexual morph: undetermined. Asexual morph: Pycnidia smooth, subglobose to ellipsoidal, hyaline. Conidia ellipsoidal to subcylindrical, pale yellow to green, smooth- and thin-walled, abundant, generated from pycnidia, aseptate, 8.5–11.7 × 4.5–7 µm (av. = 9.9 ± 0.9 × 5.4 ± 0.6 µm, n = 30). Mycelia lateral branching, septate, hyaline.

Culture characteristics.

Colonies on PDA reaching 42–45 mm diam. after 7 days 28 ° C in the dark, margin regular, aerial mycelium flat, whitish; reverse black to pale buff. Pycnidia and conidia produced on the colony surface after being cultured for 14 days at 28 ° C in the dark. Colonies on OA reaching 34 – 39 mm diam. after 7 days at 28 ° C in the dark, margin irregular, aerial mycelium flat, black in the centre, white edges; reverse concolorous.

Materials examined.

China, Yunnan Province, Puer City , Jingdong Yizu Autonomous County, from healthy leaves of C. sinensis , 13 Jun 2020, Y. C. Wang, Holotype HMAS 352391 , culture ex-type CGMCC 3.24253 = YCW 1883 .

Notes.

Neoascochyta yunnanensis is closely related to N. rosicola with high statistical support (99 % / 1, ML / PP, Fig. 4 ). Neoascochyta yunnanensis has 2 bp differences in ITS from N. rosicola . In the present study, one strain was isolated from healthy tea plant leaves. It was isolated and identified from tea plant for the first time.

Figures

Figure 4. Phylogenetic tree generated by Maximum Likelihood analysis, based on the combined ITS, LSU, RPB 2 and TUB 2 dataset of Neoascochyta, Paraboeremia, Remotididymella and Stagonosporopsis species. Bootstrap support values above 50 % and Bayesian posterior values above 0.75 are shown at each node (ML / PP). Pleiochaeta setosa CBS 118.25 / CBS 496.63 is used as the outgroup. Ex-type strains are emphasised in bold.

Figure 9. Neoascochyta yunnanensis YCW 1883 A, B colony on PDA (front and reverse) C, D colony on OA (front and reverse) E mycelia F pycnidia forming on PDA G conidia. Scale bars: 10 μm (E – G).