Pseudokiefferiella

Krasheninnikov, Andrey B., Makarchenko, Eugenyi A., Semenchenko, Alexander A., Gavrilo, Maria V. & Vshivkova, Kristina A., 2020, Morphological description and DNA barcoding of some Diamesinae (Diptera Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic), Zootaxa 4802 (3), pp. 587-600 : 595

Pseudokiefferiella sp.

( Fig. 12 )

Pseudokiefferiella sp.|Male, available from:

http://v3.boldsystems.org/index.php/Public_RecordView?processid=CHRFI378-11

GenBank accession number: JN266675 , available from :

https://www.ncbi.nlm.nih.gov/nuccore/ JN266675

Material examined. 1 adult male, RUSSIA: Vaigach Island , Lyamchin Peninsula, stream in the vicinity of Bolshoy Lyamchin Nos Cape, N 69.860028 E 59.133417, 07.VIII.2015, leg. A. Krasheninnikov ; 1 adult male, the same location except, mouth part of the stream from the walrus rookeries, N 69.856278 E 59.132917, 07.VIII.2015, leg. A. Krasheninnikov ; 1 adult male, the same locality except, stream, N 69.855972 E 59.163417, 07.VIII.2015, leg. A. Krasheninnikov ; 1 adult male, the same locality except, puddles on the shore of the Okhotnich’ya sea lip, N 69.88425 E 59.357556, 07.VIII.2015, leg. A. Krasheninnikov.

Adult male (n=4). Total length 3.4–4.1 mm. Wing length 2.3–2.7 mm. Total length/wing length 1.25–1.71. Wing length/length of profemur 2.73–3.0.

Сolouration. Total colour brown, wings greyish.

Head. Eyes pubescent, slightly elongate dorsomedially. Temporal setae 15–22, including 10–16 verticals and 5–6 postorbitals. Clypeus with 0–2 setae. Antenna with 13 flagellomeres and well-developed plume; terminal flagellomere with 1 subapical seta 30 μm long; AR 1.31–1.54. Palpomeres lengths (in μm): 31; 47; 125–140; 109–125; 94–109. Palpomeres 1–5 length/head width 0.70–0.79.

Thorax. Antepronotum with 3–5 ventrolateral setae. Dorsocentrals 9–10, prealars 5–6, scutellars 7–9.

Wing. R with 6–20 setae, R 1 with 2–4 setae in basal part; R 2+3 with 1 pore, R 4+5 with 0–1 seta. Costa extension 47–49 μm long. RM length/MCu length 2.70. Brachiolum with 1–2 setae.Anal lobe developed, rounded and slightly protrude. Squama with 24–31 setae 80–104 μm long. VR 0.86–0.98.

Legs. Spur of fore tibia 62–78 μm long; both spurs of mid tibia 47 μm long; of hind tibia 62 μm and 30 μm long. Hind tibial comb with 12 setae. Lengths and proportions of leg segments as in Table 5.

Hypopygium ( Fig. 12 ). Tergite IX with 11–18 setae (from one side) and with anal point 62–78 μm long, which in distal half slender and hair-like. Laterosternite IX with 5–8 setae. Transverse sternapodeme like narrow arch, 170–180 μm long. Gonocoxite 250–280 μm long, with broad basal lobe and lobe-like medial field, covered with 6–9 setae 24–32 μm long. Gonostylus 110–140 μm long, with long and rounded apically crista dorsalis; macroseta 8–9 μm long. HR 2.00–2.28.

Comments. After comparing the male hypopygium and DNA analysis data of the Pseudokiefferiella sp. from Vaigach Island and the Pseudokiefferiella sp. from Northern Norway (BOLD ID: CHRFI378, BIN BOLD:AAV2899) we came to the conclusion that this is the same and apparently new to science species. However, we believe that in order to fully confirm this it is necessary to conduct an additional revision of the known species of this genus shown in Figure 17 . It is also necessary to determine which of them is really the Pseudokiefferiella parva (Edwards) .

Figures

FIGURE 17. Bayesian tree based on mitochondrion COI gene for the genus Pseudokiefferiella Zavřel. SYM+G, F81 and HKY+G was the best models of nucleotide substitution for the first, second and third of COI codon position respectively. Bayesian posterior probabilities (PP) are given above tree nodes and bootstrap support values found in the ML analysis are shown below nodes. Specimens obtained in this study are in bold.

FIGURES 8–12. Diamesa urvantsevi sp. nov. (8–10), D. amplexivirilia Hansen (11) and Pseudokiefferiella sp. (12), males. 8, 10–12, hypopygium in dorsal view; 9, “anal point”. ap—“anal point”.