Colletotrichum chiangraiense X.Y. Ma, K.D. Hyde & Jayawardena

Ma, Xiaoya, Nontachaiyapoom, Sureeporn, Jayawardena, Ruvishika S., yde, Kevin D., Gentekaki, Eleni, Zhou, Sixuan, Qian, Yixin, Wen, Tingchi & Kang, Jichuan, 2018, Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand, MycoKeys 43, pp. 23-57 : 30

Colletotrichum chiangraiense X.Y. Ma, K.D. Hyde & Jayawardena sp.nov. Figure 3

Etymology.

In reference to the host sample site Chiang Rai, Thailand.

Holotype.

MFLU 17-1201 (ex-holotype culture: MFLUCC 14-0119).

Description.

Asexual morph not observed.

Sexual morph on CMA. Vegetative hyphae 4.6. ± 1.8 µm diam (N=20), hyaline to pale brown, smooth-walled, septate, branched. Chlamydospore globose, brown. Hyphae fusion and crozier observed. Ascomata rare, covered by mycelia, black. Appressoria (14.4-) 15.3-19.6 (-20.5) × (7.4-) 7.3-7.9 (-8) µm (N=2), single, outline ampulliform or ovate, pale brown. Asci (30.7-) 33.4-52.7 (-72.18) × (5.7-) 6.5-8.2 (-9.4) µm (N=15), cylindrical, straight to curved, unitunicate, 8-spored. Ascospores (11-) 11.9-15.4 (-16.7) × (2.2-) 2.8-3.8 (-4.4) µm (N=20), L/W = 4.2, bi-seriately, smooth-walled, cylindrical or fusiform, one guttule in the middle, hyaline.

Cultures on CMA flat with entire margin. Growth rate: 0.6cm/day, with 20-days for sporulation. Fluffy, dark green in the middle and white margin, reverse black in the middle.

Material examined.

Thailand, Chiang Rai, Wat Phra That Doi Tung (Temple of Doi Tung Pagodas), the host Dendrobium sp. 2 was collected on 19 December 2013, Collector: Sureeporn Nontachaiyapoom, Natdanai Aewsakul, Xiaoya Ma.

Notes.

Colletotrichum chiangraiense (MFLUCC 14-0119) formed a single branch with the support of 81ML/1.00BI/78MP in boninense species complex. It has 125 different base pairs (mainly in ITS and ACT) from the closest strain C. cymbidiicola . Blastn searches with sequences of MFLUCC 14-0119 resulted in 99% identity with ITS of the endophytic C. crassipes strain DO93 (GenBank KP050648) isolated from Dendrobium officinale in China (Unpublished), 99% identity with ACT of the endophytic Colletotrichum sp. strain COAD 2105 (GenBank KY407893) isolated from Cattleya jongheana in Brazil (Unpublished), 98% identity with TUB2 of the endophytic C. boninense strain CBS 125502 (GenBank KJ955336) isolated from Camellia sinensis in China ( Liu et al. 2015) and 98% identity with TUB2 of the endophytic C. boninense strain CGMCC 3.15165 (GenBank KC244156) isolated from Bletilla ochracea in China ( Tao et al. 2013). This species was observed antheridium, mycelia fusion and crozier, which indicates that this species may be homothallic. Here we introduce the strain isolated from root of Dendrobium sp. 2 as a new species.