Distoseptispora thysanolaenae Goonas., Dayarathne, Phookamsak & K.D.Hyde, in Phookamsak et al., Fungal Diversity 95: 126 (2019)

Shen, Hong-Wei, Bao, Dan-Feng, Hyde, Kevin D., Su, Hong-Yan, Bhat, Darbhe J. & Luo, Zong-Long, 2021, Two novel species and two new records of Distoseptispora from freshwater habitats in China and Thailand, MycoKeys 84, pp. 79-101 : 79

Distoseptispora thysanolaenae Goonas., Dayarathne, Phookamsak & K.D.Hyde, in Phookamsak et al., Fungal Diversity 95: 126 (2019)

Figure 5

Description.

Saprobic on submerged wood in freshwater River. Sexual morph: Undetermined. Asexual morph: Colonies on the substratum superficial, effuse, scattered, hairy, dark brown. Mycelium partly immersed, composed of branched, septate, smooth, brown to dark brown hyphae. Conidiophores 41-59 × 4-5 μm (x¯ = 50 × 5 μm, n = 20) macronematous, mononematous, unbranched, single, erect, straight or slightly curved, smooth, 3-6-septate, pale brown to brown. Conidiogenous cells monoblastic, integrated, determinate, terminal, dark brown, cylindrical. Conidia 46-87 × 9-12 μm (x¯ = 67 × 10 μm, n = 25) acrogenous, solitary, dry, smooth, obclavate, elongated, straight or slightly curved, truncate at base, tapering towards apex, 6-19-septate, dark grayish-brown to light yellow-green, thick-walled.

Culture characteristics.

Conidia cultivated on PDA within 12 h and germ tubes produced at the apex. Colonies on PDA, reaching 6 cm after 6 weeks at room temperature (25 ℃). Mycelium loose, flocculent, neat edges, convex in middle, pale brown to dark brown. Black, smooth on the back.

Material examined.

China, Yunnan Province, Lushui City, Nujiang River , 26°23'12"N; 98°53'94"E, on submerged decaying wood, 3 May 2016, Zonglong Luo and Songming Tang, S-876 (KUN-HKAS 112710), living culture (DLUCC 876 = KUNCC 21-10710)

Notes.

Our new collection is identical to Distoseptispora thysanolaenae in characters of the conidiophores, conidiogenous cell, and conidia ( Phookamsak et al. 2019). Furthermore, our new isolate phylogenetically clusters with the ex-type strain of D. thysanolaenae (KUN-HKAS 102247) with 100%ML/1.00PP support (Figure 1 ). Distoseptispora thysanolaenae was collected from terrestrial habitats in China, while, our new isolate was collected from freshwater habitat in China. Therefore, we identified our new collection as D. thysanolaenae , and it is new to freshwater habitats in China.

Figures

Figure 1. Maximum likelihood (ML) tree is based on combined of LSU, ITS, SSU, TEF 1 - α, and RPB 2 sequence data. Bootstrap support values with an ML greater than 70 % and Bayesian posterior probabilities (PP) greater than 0.97 given above the nodes, shown as " ML / PP ". The tree is rooted with Pseudostanjehughesia aquitropica (MFLUCC 16 - 0569) and P. lignicola (MFLUCC 15 - 0352). New species are indicated in red and type strains are in bold.

Figure 5. Distoseptispora thysanolaenae (KUN-HKAS 112710) A colonies on the substratum B-D conidiophores with conidia E, F conidiogenous cells G-N conidia O germinating conidium P, Q culture on PDA Scale bars: 30 μm (B-D); 10 μm (E, F); 20 μm (G-O).