Diplodia seriata De Not., Micr. Ital. Dec. 4: 6. (1942).

Li, Wen-Li, Liang, Rui-Ru, Dissanayake, Asha J. & Liu, Jian-Kui, 2023, Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China, MycoKeys 97, pp. 71-116 : 71

Diplodia seriata De Not., Micr. Ital. Dec. 4: 6. (1942).

Fig. 11

Description.

Saprobic on decaying branches of Camellia oleifera . Sexual morph: Ascomata 301-343 × 293-340 (x̄ = 322 × 316 μm, n = 10), more or less subglobose, solitary or gregarious, semi-immersed, medium brown to dark brown, unilocular, papillate, ostiolate. Ostiole 72-78 μm diam., conical or circular, central, papillate, periphysate. Peridium 33-44 μm wide, composed of dark brown, 4-6 layers of textura angularis. Pseudoparaphyses 2-2.5 μm wide, hyaline, branched, septate. Asci 112-141 × 27.5-30 μm (x̄ = 126 × 28.5 μm, n = 30), clavate, stipitate, bitunicate, containing eight, biseriate ascospores. Ascospores 31.5-32.5 × 12-13.5 μm (x̄ = 32 × 13 μm, n = 30), L/W ratio = 2.5, broadly fusiform to oval, widest in the middle, both ends obtuse, hyaline, moderately thick-walled, smooth, becoming brown when aged. Asexual morph: Not observed.

Culture characteristics.

Ascospores germinate on PDA within 12 h. Colonies growing on PDA, reaching a diameter of 4 cm after five days at 25 °C, effuse, velvety, with entire to slightly undulate edge.

Material examined.

China, Sichuan Province, Jiangyou City, shuanghe County , 31°54'10"N, 104°55'57"E, elevation 656 m, on dead branches of Camellia oleifera , 10th June 2021, W.L Li, 288 (HUEST 22.0073), living culture UESTCC 22.0072 .

Notes.

The morphology of the taxa isolated from decaying woody oil plants is similar to Diplodia seriata . In the multi-gene phylogenetic analysis, our new collection clustered with the ex-type strain of Di. seriata (CBS 112555) with strong bootstrap support. Diplodia seriata has been isolated from a wide range of hosts (121 species) and has a worldwide distribution (reported in 46 countries) ( Batista et al. 2021). This is the first report of Di. seriata isolated from Camellia oleifera .

Figures

Figure 11. Diplodia seriata (HUEST 22.0073, new host record) a-c appearance of ascomata on natural substrate d vertical section of ascomata e section of peridium f-i asci j-n ascospores. Scale bars: 100 μm (d); 10 μm (e, j-n); 20 μm (f-i).