Cylindrocladiella solicola N.Q. Pham, T.Q. Pham & M.J. Wingf.

Pham, Nam Q., Barnes, Irene, Chen, ShuaiFei, Pham, Thu Q., Lombard, Lorenzo, Crous, Pedro W. & Wingfield, Michael J., 2018, New species of Cylindrocladiella from plantation soils in South-East Asia, MycoKeys 32, pp. 1-24 : 8

Cylindrocladiella solicola N.Q. Pham, T.Q. Pham & M.J. Wingf. sp. nov. Figure 6

Etymology.

Name refers to soil, the substrate from which this fungus was first isolated.

Type material.

VIETNAM. Tuyen Quang Province, from soil in Acacia hybrid plantation, Nov. 2013, N.Q. Pham & T.Q. Pham, herbarium specimen of dried culture, PREM 62164 (holotype), CMW 47198 = CBS 143551 (ex-type culture).

Description.

Sexual morph not observed. Conidiophores dimorphic, penicillate and subverticillate, mononematous and hyaline. Penicillate conidiophores comprising a stipe, a penicillate arrangement of fertile branches, a stipe extension and a terminal vesicle; stipe septate, hyaline, smooth, 58.5-120 × 2.5-5 µm; stipe extension aseptate, straight, 93.5-170 µm long, thick-walled with one basal septum, terminating in thin-walled, broadly clavate to lanceolate to fusiform vesicles, 3.5-6.5 µm wide. Penicillate conidiogenous apparatus with primary branches aseptate, 16-36.5 × 3-4.5 µm, secondary branches aseptate, 10-16 × 2.5-3.5 µm, each terminal branch producing 2-4 phialides; phialides cymbiform to cylindrical, hyaline, aseptate, 9-15.5 × 2-3 µm, apex with minute periclinal thickening and collarette. Subverticillate conidiophores abundant, comprising of a septate stipe and primary branches terminating in 2-4 phialides; primary branches straight, hyaline, 0-1-septate, 16.5-25 × 2.5-5 µm; phialides cymbiform to cylindrical, hyaline, aseptate, 12-28 × 2.5-4 µm, apex with minute periclinal thickening and collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, (10.5 –)12.5–14.5(– 15.5) × 2 –2.5(– 3) µm (av. = 13.5 × 2.5 µm), frequently slightly flattened at the base, held in asymmetrical clusters by colourless slime.

Culture characteristics.

Colonies honey to isabelline on the surface and sepia to brown vinaceous in reverse on MEA after 7 d; undulate margins; extensive aerial mycelium especially in the middle; chlamydospores moderate, arranged in chains. Optimal growth temperature at 25 °C, no growth at 5 °C and 35 °C; after 7 d, colonies at 10 °C, 15 °C, 20 °C, 25 °C and 30 °C reached 5.2 mm, 20.4 mm, 37.8 mm, 61.2 mm and 37.1 mm, respectively.

Distribution.

Tuyen Quang, Vietnam

Notes.

Cylindrocladiella solicola is phylogenetically closely related to Cy. malesiana , Cy. microcylindrica and Cy. natalensis . The stipe extensions of Cy. solicola are longer than those of Cy. malesiana , Cy. microcylindrica and Cy. natalensis (Table 2).