Pyropia raulaguilarii Mateo-Cid, Mendoza-González & Sentíes, 2012

Mateo-Cid, Luz Elena, Mendoza-González, Angela Catalina, Larrea, Jhoana Díaz-, Sentíes, Abel, Pedroche, Francisco F. & Heredia, Juan Diego Sánchez, 2012, A new species of Pyropia (Rhodophyta, Bangiaceae), from the Pacific coast of Mexico, based on morphological and molecular evidence, Phytotaxa 54 (1), pp. 1-12 : 3-6

Pyropia raulaguilarii Mateo-Cid, Mendoza-González & Sentíes , sp. nov. ( Figs 1–8 , Table 2)

Gametophytum foliosum, monostromaticum, lanceolatum, basis umbilicalis , margo crispata. Thalli 4 ad 6 cm, adhaerentes perfecte ad papyrum. Thalli steriles usque 40–42 µm crassi. Color rubrum ad aliquando purpureus. Thalli sexuales monoecii spermatangia in viridi inaequalis intermixtam zygotosporangia in rectangulum vel irregularis inaequaliter. Sectiones fertiles (feminei) 50–55 µm crassi. Structurae reproductivae marginales, maculis extensiae intro. Spermatangia in sarcinula 2 × 2 × 4 × 4. Zygotosporangia in sarcinula 2 × 2 × 4.

Type: — MEXICO. Michoacán: Carrizalillo, low deciduous forest, 18°36’00” N, 102°33’03” W, 0 m a.s.l., coll. Mendoza-González and Mateo-Cid , 04-04-2009, MICH-09- A/01 (holotype: ENCB!, isotype: UAMIZ!, paratype: Michoacán: Caletilla, 18°03’28” N, 102°42’13” W, coll. Mendoza González and Sánchez Heredia, 17-05-2006, MICH-06-30/01, ENCB!) .

Gametophyte foliose, monostromatic, lanceolate, rarely branched ( Fig. 1 ), often deeply ruffled, margin undulating ( Fig. 3 ). Thalli 4–6 cm high, blades 1–2 cm diameter, red to purple color. Adhering completely to paper. The plants have a well-developed umbilicate base, which is closely adherent to the substratum and attached by rhizoidal cells ( Fig. 2 ). Sterile thalli 40–42 µm thick ( Fig. 4 ), vegetative cells measuring 14–17 µm in diameter and 20–25 µm in length. Vegetative cells with a single stellate plastid with one pyrenoid. Reproductive plants: sexual thalli monoecious, spermatangia in green patches intermixed with zygotosporangia in rectangular or irregular patches in fertile regions of the blades. Fertile female sectors 50– 55 µm thick ( Fig. 5 ). Reproductive structures marginal, extending inward in alternating patches. Submarginal tissue paler than the adjacent outer zygotosporangial and inner sterile tissues. Zygotosporangia in packets of 2 × 2 × 4 ( Figs 7, 8 ). Spermatangia in packets of 2 × 2 × 4 × 4 ( Fig. 6 ).

Habitat: —On rocks, intertidal pools.

Etymology: —The species epithet is named in honor of Prof. Raúl Aguilar Rosas, a prominent Mexican phycologist and beloved colleague from Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Mexico.

Molecular analyses: —Samples of Pyropia raulaguilarii from Carrizalillo and Caletilla (Michoacán, Mexico) diverged each other 0.02–0.03%. These samples diverged 3.0 to 3.4% from the GenBank sequence called Pyropia cf. thuretii from Mazatlán, Mexico ( Table 1). The concatenated phylogenetic data set consisted of 62 taxa ( Table 1), including 59 ingroup and three outgroup taxa, with 2767 characters: 1300 from the nrSSU and 1467 from the rbc L gene. Bayesian, parsimony (not shown) and distance (not shown) analyses resulted in similar trees. The topology of the Bayesian tree showed eight lineage corresponding to representatives of the following Bangiales genera: Bangia Lyngbye , Boreophyllum , Clymene , Fuscifolium , Lysithea , Porphyra C.Agardh , Pyropia and Wildemania ( Fig. 9 ). The Pacific Mexican samples processed in the present study were located in two well-supported terminal Pyropia sister clades. The samples collected in Carrizalillo and Caletilla (Michoacán) formed a high supported monophyletic clade with a sister clade called Pyropia cf. thuretii from Mazatlán, Mexico ( Table 1).

analysis.

Figures

FIGURE 9: Bayesian result for concatenated nuclear SSU ribosomal RNA (nrSSU) and rbcL data set. Support values are Bayesian posterior probabilities, and parsimony and distance bootstrap values, respectively. Some internal support values are omitted for clarity.

FIGURES 1–8: Pyropia raulaguilarii sp. nov. Fig. 1: Habit of the foliose gametophyte. Holotype. El Carrizalillo (ENCB 19 603). Fig. 2: Rhizoidal cells in surface view. Fig. 3: Margin of thallus in surface view. Fig. 4: Transverse section through thallus showing monostromatic blade. Fig. 5: Transverse section of female portion. Fig. 6: Mature spermatangia in surface view. Fig. 7: Surface view of zygotosporangial region of thallus. Fig. 8: Mature marginal zygotosporangias in surface view. Scale bars: Fig. 1: 12 mm; Figs 2, 6: 30 µm; Figs 3, 7, 8: 40 µm; Fig. 4: 15 µm; Fig. 5: 20 µm.