Leptomyrmex relictus, Brendone Boudinot, Rodolfos Probst, Carlos Roberto F. Brandão, Rodrigo Feitosas & Philip S. Ward, 2016

Brendone Boudinot, Rodolfos Probst, Carlos Roberto F. Brandão, Rodrigo Feitosas & Philip S. Ward, 2016, Out of the Neotropics: newly discovered relictual species sheds light on the biogeographical history of spider ants (Leptomyrmex, Dolichoderinae, Formicidae), Systematic Entomology 41, pp. 658-671: 663-667

publication ID

http://doi.org/ 10.1111/syen.12181

publication LSID

lsid:zoobank.org:pub:6E9E6617-6E53-40B8-82C7-67F89A83C553

DOI

http://doi.org/10.5281/zenodo.5670018

persistent identifier

http://treatment.plazi.org/id/936F5440-9B94-43C0-95ED-2099FD380471

taxon LSID

lsid:zoobank.org:act:936F5440-9B94-43C0-95ED-2099FD380471

treatment provided by

Plazi

scientific name

Leptomyrmex relictus
status

sp.n.

Leptomyrmex relictus   sp.n.

http://zoobank.org/urn:lsid:zoobank.org:act: 936 F 5440 - 9 B 94 - 43 C 0-95 ED- 2099 FD 380471 View Materials

( Figs. 1–5 View Fig. 1 View Fig. 2 View Fig. 3 View Fig. 4 View Fig. 5 , Figures S 1, S 2)

Type material. Holotype worker. BRAZIL, Goiás: Niquelândia , Serra da Mesa , 14 ∘ 20 ′ 39.5 ′′S, 48 ∘ 10 ′ 21.1 ′′W, 16.xi. 2013, cerrado, inside nest of Cornitermes silvestrii (D. E. Oliveira, R. G. Santos & T. Carrijo)   (sample code DEO 2013 -030; specimen code CASENT0106501) [ MZSP]. GoogleMaps  

Paratype workers (w) and males (m). Same data and colony as holotype; sample code R06 [1 w MZSP, 1 w ( CASENT0730400) 1 m UCDC]; sample code TC08 [1 w DZUP, 1 w MZSP]; sample code DEO 2013 -030 [1 w LACM, 1 m MZSP].  

Additional material examined (nontype): 12 w, 6 m, 3 larvae, and 2 pupae from 6 localities in Goiás and Tocantins, Brazil ( Fig. 2 View Fig. 2 ).

Diagnosis. With diagnostic characters of genus from Shattuck (1992), notably with medial hypostomal notch (synapomorphy of genus) and lacking pterostigmal appendage in male (synapomorphy of macro- Leptomyrmex   ). Distinguished from all Leptomyrmex   by the following characters: (i) short, thick, erect setae present on the head capsule, mesosoma (legs included) and metasoma ( Figs 1 View Fig. 1 A, 3 A, B); (ii) clypeal setae comparatively more numerous ( Figs 1 View Fig. 1 E, 3 B); (iii) anterior clypeal margin strongly convex ( Figs 1 View Fig. 1 E, 3 B); (iv) lateral hypostoma weakly flanged; (v) worker with convexity subtending subapical mandibular tooth ( Fig. 1 View Fig. 1 E); (vi) worker petiole lateromedially narrow; (vii) worker gaster strongly lateromedially compressed; (viii) male scape elongate (SI 1.26–1.40 versus 0.19–1.05 in other Leptomyrmex   ); (ix) male wing with five closed cells (costal, basal, subbasal, submarginal 1, marginal 1) ( Fig. 5 View Fig. 5 E); (x) male petiole dorsal margin concave ( Fig. 3 View Fig. 3 A); (xi) male petiolar spiracles situated on tubercles ( Fig. 3 View Fig. 3 A); (xii) several features of male genitalia ( Fig. 3 View Fig. 3 C–K; see Appendix S 2 for description). Characters 1 –5, 9– 11 and those of 12 are unique in the genus.

Biology. Although males of L. relictus   have been collected as far back as 1956 – remaining unidentified for over half a century – workers were only recently discovered in 2013 from nest samples of Cornitermes silvestrii Emerson   and in 2012 from epigaeic and arboreal pitfall traps in typical cerrado (Brazilian savannah) habitat. It is unknown whether workers are nocturnal or diurnal, but hypothetical nocturnal habits and/or their Camponotus   -like habitus may account for the low encounter rate of this species. It is interesting to note that at least three of the specimens were captured in arboreal pitfall traps, suggesting that workers may forage both on the soil and in the vegetation strata. The collection of workers ( Fig. 1 View Fig. 1 A, E) and brood (Figure S 1) inside termitaria suggests that L. relictus   may live in association with Cornitermes silvestrii   . One termite nest measuring 1.30 m wide and about 40–50 cm tall inhabited by L. relictus   was found in northeast Goiás state under a tree, and was also occupied by another ant species, Camponotus blandus (Smith, F.)   , which is known to be a facultative termite inquiline ( Gallego-Ropero & Feitosa, 2014). The other termite nest with L. relictus   specimens was found in southeast Tocantins state, along a trail edge. Unfortunately, no data are available on how the Leptomyrmex   occupy the termitaria or if they were sharing galleries with the termites. A potential association with C. silvestrii   is compelling as the termitaria of C. silvestrii   are large, environmentally-stable clay mounds and may house several other ant and termite species (see Appendix S 2). In addition to describing and comparing the sociometric and sociogenic patterns ( Tschinkel, 1991, 2011) of L. relictus   with the Australian species, future studies should test the association of the species with C. silvestrii   .

Phylogeny. Phylogenetic analyses of the 10 -gene dolichoderine data matrix yielded results congruent with those of Ward et al. (2010) ( Fig. 4 View Fig. 4 ). In particular, we consistently recovered all four dolichoderine tribes, with Tapinomini   as sister to all other Dolichoderinae   . Leptomyrmex relictus   is positioned as sister to the six Australian Leptomyrmex   with strong support: Bayesian posterior probability (PP) of 0.96, and ML bootstrap support ( MLBS) of 93 %. There is maximum support (PP 1.00, MLBS 100 %) for the Australian Leptomyrmex   and for the micro- and macro- clades within it. Our results differ from those of Lucky (2011) for the internal topology of Leptomyrmex   in that, with our sampling, L. unicolor   is sister to L. erythrocephalus   , rather than L. geniculatus   + L. rufithorax   . A sister-group relationship between Leptomyrmex   and the ( Forelius Emery   + Dorymyrmex Mayr   ) clade is also sustained (PP 1.00, MLBS 74 %). Finally this larger clade of ( Leptomyrmex   + ( Forelius   + Dorymyrmex   )) is sister to all other Leptomyrmecini   , as in Ward et al. (2010). Notably, L. relictus   is a long-branched taxon; because its divergence from the most recent common ancestor of the crown Leptomyrmex   , it is apparent that L. relictus   has undergone considerable morphological (especially genitalic) and molecular evolution.

Divergence dating. Two alternate analyses were run treating † Chronomyrmex   as a stem dolichoderine, reflecting uncertainty of stem or crown placement of † L. neotropicus   with respect to extant Leptomyrmex   . Dating results obtained were robust to alternate placements of † L. neotropicus   . The chronogram from the stem Leptomyrmex   treatment is presented in Fig. 5 View Fig. 5 . Mean ages between the two treatments for the clades listed in Table S 3 differed by an average of 0.21 Ma, and 95 % highest probability densities (hpd) differed by an average of 0.26 Ma at the older and 0.53 Ma at the younger ends, respectively. These findings are in accord with results of Ward et al. (2010) and Lucky (2011) (Table S 3), although less so with the latter which incorporated fewer outgroups and loci. Only the dolichoderomorph crown age differed notably from Ward et al. (2010), being 11 Ma younger. This difference is attributable to the modified dolichoderomorph root node calibration used herein which is a diffuse normal distribution, not restricting the minimum age of the node to 100 Ma with the unplaceable yet putative aneuretine fossil † Burmomyrma   .

Biogeographical history. The LAGRANGE analysis was run with a single outgroup taxon ( Aneuretus simoni Emery   ) ( Fig. 5 View Fig. 5 ), and all results are presented in Table S 4. A Neotropical origin was recovered with high support (relative probabilities> 0.80) for the Leptomyrmecini   , the Leptomyrmex   + ( Dorymyrmex   + Forelius   ) clade and Leptomyrmex   ( Fig. 5 View Fig. 5 A). The crown ages of Leptomyrmex   and the Australasian clade are estimated to be 46 Ma (95 % hpd 53 – 37 Ma) and 28 Ma (95 % hpd 40 to 18 Ma), respectively (Table S 3, calibration 2). We therefore infer dispersal to Australia between those two time periods. This range expansion probably happened before the breakup of the Antarctic land bridge, estimated to have occurred about 35 Ma for Australia and Antarctica, and about 30 to 28 Ma for Antarctica and South America ( Lawver et al., 1992; Sanmartín & Ronquist, 2004). Another notable result is an inferred Neotropical origin for the Dolichoderini   (comprising solely Dolichoderus Lund   ) ( Fig. 5 View Fig. 5 A). Only four nodes had relative probabilities <0.33 for inferred ancestral ranges: the crown and root Dolichoderinae   , the BDL ( Bothriomyrmecini   + Dolichoderini   + Leptomyrmecini   ) clade, and the Bothriomyrmecini   . All other relative probabilities were> 0.66, except the Liometopum   + Aptinoma Fisher   + Tapinoma Foerster   clade, which had a relative probability between 0.33 and 0.66. Further sampling within the Bothriomyrmecini   is expected to increase the relative probabilities of inferred ancestral ranges for the tribe as well as the BDL clade.

Results and evaluation of fossil record. Dlussky et al. (2014) claimed that † Usomyrma   , † Leptomyrmula   and Leptomyrmex   form a natural group based on the putatively synapomorphic ‘reduction of the central vein sections and cells.’ This is refuted by the retention of complete wing venation in the species of micro- Leptomyrmex   , which is nested well within the genus. Moreover, the venation reduction patterns of † Usomyrma   , Dorymyrmex   + Forelius   and Leptomyrmex   are not homologous. † Usomyrma   has lost Rs + M and Mf 2-3; the former is autapomorphic and the latter has occurred at least five times in the Dolichoderinae   ( Fig. 5 View Fig. 5 ). At least two reductions have occurred in Leptomyrmex   ( Fig. 5 View Fig. 5 ) as the micro- clade retains complete wing venation, whereas L. relictus   , the macro- Leptomyrmex   and † L. neotropicus   have differing reduction patterns. Leptomyrmex relictus   has lost 1 m-cu and Mf 2-3, † L. neotropicus   has lost Rsf 2 +, Mf 2 + m, 2 rs-m, 1 m-cu and 2 r-rs, whereas macro- Leptomyrmex   have lost Mf 2 -3, 2r-rs (variable) and 1 m-cu, and retained Rs + M, Rsf 2 + and Mf 4 +. Other characters claimed by Dlussky et al. (2014) to support a relationship between † Usomyrma   and † Leptomyrmex   are, by their explicit statement, either plesiomorphic or autapomorphic; these are thus uninformative of relationships by definition. Furthermore, based on examination of a paratype male, † Usomyrma   lacks a newly discovered genitalic synapomorphy of the Leptomyrmecini   : a dorsal sclerotic bridge between the penisvalvar bases. The sclerotic bridge is anteroposteriorly broad and fused with the basimeres in L. relictus   ( Fig. 2 View Fig. 2 C, F), and is narrowed anteroposteriorly in the Australasian clade. The strap- or thread-like condition of the bridge in the Australasian clade is a synapomorphy, whereas fusion of the bridge to the basimeres in L. relictus   is autapomorphic.

Regarding † Leptomyrmula   specifically, the only known specimen may be lost (G.M. Bargossi, University of Bologna, Italy, personal communication), but it has fortunately been illustrated ( Emery, 1891) (reproduced in Fig. 5 View Fig. 5 H). The pattern of vein abscissa loss is most similar between † Leptomyrmula   and the macro- clade ( Fig. 5 View Fig. 5 F), with the venation being clearly different from the micro- Leptomyrmex   ( Fig. 5 View Fig. 5 A) and L. relictus   ( Fig. 5 View Fig. 5 E), which have complete or relatively complete wing venation, respectively. Homology of the loss pattern between † Leptomyrmula   and the macro- Leptomyrmex   is unlikely, however, given the distinctions of the venational patterns. Forewing characters of † Leptomyrmula   differ as follows from the macro- Leptomyrmex   clade: costal cell broad and short (vs. absent, with secondary sclerotization); free R lacking along the anterior wing margin (vs. continuing almost to wing apex); pterostigmal appendage absent (vs. present); 2 rs-m + Mf 4 + absent (vs. usually present); basal cell narrow and Mf 1 subparallel to Sc + R + Rs (vs. broad, Mf 1 and Sc + R + Rs clearly converging); free Cu short and diverging from M + Cu in the basal third of the wing (vs. about midlength); and anal vein elongated after crossvein cu-a (vs. anal vein strongly reduced or absent). Moreover, the somatic morphology of † Leptomyrmula   is considerably divergent, including the following differences: head capsule dorsoventrally compressed; compound eyes small and anteriorly-situated; mandibles small; flagellomeres short; pronotum dorsoventrally tall; mesonotum flat; and limbs short (metatibia not exceeding metasomal apex). Neither † Leptomyrmula   nor † Usomyrma   have the key macro- Leptomyrmex   clade forewing synapomorphies of the pterostigmal appendage ( Lucky & Ward, 2010) and the absent costal vein with secondary sclerotization. We therefore consider † Leptomyrmula   and † Usomyrma   incertae sedis in the Dolichoderinae   . The placement of † Leptomyrmula   in the Dolichoderinae   itself could be questioned because the genitalia, as illustrated in Emery (1891), do not conclusively demonstrate a synapomorphic condition of dolichoderine genitalia: enlarged basimere and reduced telomere ( Yoshimura & Fisher, 2011; Boudinot, 2015).

Study of worker and male † L. neotropicus   specimens revealed presence of the hypostomal notch, a synapomorphy of the genus almost completely unique in the Dolichoderinae   , except for the enigmatic leptomyrmecine Anillidris Santschi   (B.E. Boudinot, personal observation), confirming placement of the fossil within the genus. Presence of the notch could not be evaluated in either † Leptomyrmula   or † Usomyrma   ; the ventral head surface of the paratype † Usomyrma   examined is obscured by a fracture within the amber. It is plausible that † L. neotropicus   is more closely related to L. relictus   than to the Australasian clade based on genitalic characters, although the placement of the Dominican amber fossil should be tested in a ‘total-evidence’ framework ( Ronquist et al., 2012 b).

MZSP

Brazil, Sao Paulo, Sao Paulo, Museu de Zoologia da Universidade de Sao Paulo

UCDC

USA, California, Davis, University of California, R.M. Bohart Museum of Entomology

DZUP

Brazil, Parana, Curitiba, Universidade Federal do Parana, Museu de Entomologia Pe. Jesus Santiago Moure

LACM

USA, California, Los Angeles, Los Angeles County Museum of Natural History

MZSP

Sao Paulo, Museu de Zoologia da Universidade de Sao Paulo

UCDC

R. M. Bohart Museum of Entomology

DZUP

Universidade Federal do Parana, Colecao de Entomologia Pe. Jesus Santiago Moure

LACM

Natural History Museum of Los Angeles County