Aedes mosquito

Mohamed, Asia H., Ali, Alaa M., Harbach, Ralph E., Reeves, R. Guy, Ibrahim, Kamal M., AhmedMohmed, Meshkat A. & Azrag, Rasha S., 2017, Aedes mosquitoes in the Republic of the Sudan, with dichotomous keys for the adult and larval stages, Journal of Natural History 51 (9 - 10), pp. 513-529 : 515

Mosquito surveillance and collection

A longitudinal surveillance study of larval and adult mosquitoes was conducted for six months during the period of May to October 2013. Inspections of larval habitats and indoor and outdoor resting adults were carried out weekly at four study sites chosen to represent agricultural and adjacent residential areas. Mosquito larvae were collected using the standard dipping method and preserved in 70% ethanol for subsequent morphological identification. Collections of resting adult mosquitoes were performed using a knock-down procedure ( World Health Organization 1995). These were carried out in early morning with prior consent from occupants. Selection of the sampled homes was random, grouped by house type (small or large), proximity to potential larval habitats (near or at a distance from such sites). This avoided sampling bias when determining mosquito densities. Also, active searches for adults in outdoor resting sites were carried out using aspirators. Adults were preserved dry for subsequent morphological identification.