Massilia neuiana BT

Han, Joo Hyun, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Lee, Byoung-Hee & Kim, Ki-eun Lee and Myung Kyum, 2020, Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea, Journal of Species Research 9 (2), pp. 85-104 : 93-95

Description of Massilia neuiana BT 239

Cells are Gram-stain-positive, non-flagellated, and rod-shaped. Colonies are pale yellow-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, positive for dextrin, D- maltose, gentiobiose, pH 6, L- rhamnose, gelatin, glyctl-L- proline, L- aspartic, L- glutamic acid, lincomycin, D- galacturonic acid, L- galactonic acid lactone, D- glucuronic acid, tetrazolium blue, D- malic acid, L- malic acid, propionic acid, acetic acid and aztreonam; weak positive for D- glucose, D- fructose, D- galactose, D- fucose, 1% sodium lactate, D- fructose-6-PO 4, L- histidine, pectin, glucuronamide, tetrazolium violet, tween 40, β -hydroxy-D, L- butyric acid, acetoacetic acid and formic acid; but negative for D- trehalose, D- cellobiolose, sucrose, D- turanose, stachyose, pH 5, D- raffinose, α - D- lactose, D- melibiose, β - methyl-D- glucoside, D- salicin, N -acetyl-D- glucosamine, N -acetyl- β -mannosamin, N -acetyl-D- galactosamin, N -acetyl neuraminic acid, 1% NaCl, 4% NaCl, 8% NaCl, D- mannose, 3-methyl-glucose, L- fucose, inosine, fusidic acid, D- serine, D- sorbitol, D- mannitol, D- arabitol, myo-inositol, glycerol, D- glucose-6-PO 4, D- aspartic acid, D- serine, troleandomycin, rifamycin SV, minocycline, L- alanine, L- arginine, L- pyroglutamic acid, L- serine, guanidine HCl, niaproof 4, D- gluconic acid, mucic acid, quinic acid, D- saccharic acid, bancomycin, p -hydroxy-pheylacetic acid, methyl pyruvate, D- lactic acid methyl ester, L- lactic acid, citric acid, α -keto-glutaric acid, bromo-succinic acid, nalidixic acid, lithium chloride, potassium tellurite, γ -amino-butryric acid, α -hydroxy-butyric acid, α -keto butyric acid, sodium butyrate and sodium bromate. In API 20NE test, positive for arginine dihydrolase, urease and esculin hydrolysis; but negative for reduction of nitrates (NO 3) to nitrite (NO 2 -), reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, gelatin hydrolysis, β -galactosidase and D- maltose (weak) were utilized. D- Glucose, D- mannose, D- mannitol, N -acetyl-D- glucosamine, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate and phenylacetic acid were not utilized. In API 32GN test, N -acetyl-glucosamine, D- ribose, inositol, D- saccharose (sucrose), itaconic acid, suberic acid, sodium malonate, lactic acid, potassium 5-ketogluconate, 3-hydroxybenzoic acid, D- mannitol, D- glucose, salicin, D- melibiose, D- fucose, D- sorbitol, capric acid, valeric acid, trisodium citrate, L- histidine, potassium 2-ketogluconate and 4-hydroxybenzoic acid were utilized whereas, L- rhamnose, D- maltose, glycogen, L- serine and L- arabinose were not utilized.

Strain BT239 (= NIBRBAC000503018) was isolated from a soil sample, Pocheon City in Gyeonggi Province, Korea.