Paraisaria cascadensis Tehan, Dooley & Spatafora, 2023

Tehan, Richard M., Dooley, Connor B., Barge, Edward G., McPhail, Kerry L. & Spatafora, Joseph W., 2023, New species and new combinations in the genus Paraisaria (Hypocreales, Ophiocordycipitaceae) from the U. S. A., supported by polyphasic analysis, MycoKeys 100, pp. 69-94 : 69

Paraisaria cascadensis Tehan, Dooley & Spatafora sp. nov.

Fig. 3

Type material.

Holotype. U.S.A., Washington. Skamania County, Gifford Pinchot National Forest, Mt. St. Helens, at approximately 46.1771, -121,9224. 1,042 m alt., 9 June 2021, on adult Cyphoderris monstrosa buried in the ground, in mixed coniferous forest comprising Pinus contorta , Pseudotsuga menziesii , and Abies sp., collected by R. Tehan, C. Dooley (RMT-2021-072, OSC-M-052017, ex-holotype living culture: ARSEF 14609.

Etymology.

cascadensis occurring in the Cascade Mountain range in the Pacific Northwest, USA.

Description.

Stroma capitate, solitary, rhizoids solitary arising from heads of adult Cyphoderris monstrosa buried in soil. Ascogenous portion globose or subglobose, 8-9 × 6-9 mm, chestnut brown. Stipe white to light brown, inside hollow, fibrous, white, 15-17 mm long, 3-4 mm wide, papillate with ostioles of perithecia. Perithecia obclavate, immersed, ordinally arranged, 800-970 × 105-150 µm. Asci hyaline, cylindrical, eight-spored, observed up to 350 µm long × 4.5-7 µm wide, possessing abruptly thickened apex. Ascospores hyaline, filiform, multiseptate, breaking into 64 cylindrical part-spores, (6.3-)7.5-9.5(-10.3) × 1.6-2.2(-2.4) µm.

Culture characteristics.

Colonies on PDA 61 days at 20 °C, 28 mm, white to yellow, reverse reddish brown to orange. Mycelium septate, smooth-walled hyaline. No conidial state was observed.

Host.

Cyphoderris monstrosa ( Prophalangopsidae , Orthoptera ).

Habitat.

Specimens occur on hypogeous adult hump-winged grigs, Cyphoderris monstrosa , in coniferous forest.

Additional materials examined.

U.S.A., Washington: Skamania County, at approximately 46.177, -121.9167, elevation: 974 m, 29 May 2018, on cf. Cyphoderris monstrosa buried in soil, collected by Josh Grefe (OSC-M-052003) . U.S.A., Washington: Chelan County, 47.9761, -120.7811, elevation: 865 m, 15 June 2020, on adult Cyphoderris monstrosa , buried in soil, collected by Daniel Winkler, Hans Drabicki (OSC-M-052010) . U.S.A., Washington: Skamania County, at approximately 46.1848, -122.1139, elevation: 12332 m, 12 June 2020, on cf. Cyphoderris monstrosa , collected by Ben McCormick (OSC-M-052012) . U.S.A., Washington: Skamania County, Gifford Pinchot National Forest, Mt. St. Helens , at approximately 46.1771, -121,9224. 1,042 m alt., 9 June 2021, on adult Cyphoderris monstrosa buried in soil, in mixed coniferous forest comprising Pinus contorta , Pseudotsuga menziesii , and Abies sp., collected by Richard Tehan, Connor Dooley (RMT-2021-071, OSC-M-052016) .

Notes.

This species is uncommon and has thus far only been collected in the Cascade Mountains of Washington State in the vicinity of Mount St. Helens at elevations above 850 m. It might be expected to have a broader range on the basis of the range of its host, Cyphoderris monstrosa , which is known to occur in coniferous forest in several Western U.S. states and Canada ( The Orthopterists’ Society 2023).

Figures

Figure 3. Paraisaria cascadensis A OSC-M- 052017 B fertile head C cross section of fertile head showing arrangement of perithecia D perithecia E ascus F Ascus apex G-I ascospores J part-spores K, L colonies on PDA 61 d (K obverse, L reverse).