Paraputo nasai, Caballero, 2021

Caballero, Alejandro, 2021, Four new scale insect species (Hemiptera: Coccomorpha) associated with coffee roots in Colombia, South America, with identification keys for genera Newsteadia Green, 1902, Distichlicoccus Ferris, 1950, and Paraputo Laing, 1929, Zoosystema 43 (18), pp. 341-363 : 352-356

Paraputo nasai n. sp.

( Figs 5 ; 6 )

urn:lsid:zoobank.org:act:7C8415A1-4443-4123-A524-EA5D0C82CBE8

TYPE MATERIAL. — Holotype. Colombia • adult ♀ (two specimens in the same slide, holotype at the rigth side indicated as “Holo” in additional label); Cauca, Páez, Vereda El Ciprés , Farm Naranjal , 2°42’31.54”N, 75°45’22.42”W; 1746 m a.s.l.; 12.XI.2015; A. Ibagón leg.; ex roots Coffea arabica (Rubiaceae) -coffee in association with Wasmannia auropunctata (Roger, 1863) ( Hymenoptera ; Formicidae ), manual; UNAB 4631 .

Paratypes. 12 adult ♀ on 7 slides • 1 ♀ (marked as “Para” shares a slide with the holotype); same data as holotype; UNAB 4631 • 5 ♀ (on three slides); same data as holotype; UNAB 4631 • 6♀ (on three slide; same data as holotype; MNHN.

ETYMOLOGY. — The specific epithet is in recognition of the aboriginal community Nasa, who inhabit the region where specimens were collected.

DIAGNOSIS. — Cerarii numbering 16 pairs; dorsal setae short, each 7-19 µm long; oral collar tubular ducts of two sizes, the smaller ones each with a diameter similar to a trilocular pore and the larger ones each with a diameter wider than that of a trilocular pore; anal bar absent; anal ring with 6 setae.

DESCRIPTION Pre-mounting specimens Appearance in situ: not observed. Specimens preserved in 75% ethanol with milky appearence; color unchanged in 10% potassium hydroxide.

Post-mounting specimens

Slide-mounted adult females (n = 8): body oval to round ( Figs 5 ; 6A ), length 1.9 ± 0.3 [1.9] (1.5-2.4) mm; wide 1.4 ± 0.3 [1.4] (1.1-1.9) mm.

Dorsum

Ostioles. Conspicuous with membranous lips and 1-8 setae and scattered trilocular pores on each lip. Anal ring. 94 ± 10.2 [84] (76-109) µm in transverse diameter, with 6 flagellate setae, each seta 89 ± 8.8 (66-104) µm long; ring containing two rows of cells, external row with cells smaller than those in internal row, subquadrate and subrectangular, each cell with a spicule 2 or 3 µm long; internal row of 72 ± 10 (59-87) cells, each cell irregular, tending to be elongate and without a spicule.

Cerarii. Numbering 16 pairs ( Fig. 5A , 6B ), C 10 absent; cerarian pattern C 1 [4-5:1-2] (2-6:1-4), C 2 [6:0] (6-10:0-3), C 3 [7-8:0] (6-12:0-3), C 4 [4-6:1-2] (4-9:0-2), C 5 [4-5:0] (3-9:0-2), C 6 [3-4:0] (3-6:0-1), C 7 [3:0] (2-3:0-2), C 8 [2:0] (1-3:0-2), C 9 [2-3:0] (1-3:0-2), C 10 absent, C 11 and C 12 [2:0] (1-3:0-1), C 13 [2:0] (1-5:0-1), C 14 [2-3:0] (2-4:0-3), C 15 [3:0] (2-4:0-2), C 16 [5-6:0-1] (3-8:0-4), and C 17 [3-4:0] (3-8:0-1); each cerarius with trilocular pores grouped on a membranous plate.

Body setae. Flagellate, each 12 ± 2.6 (6-22) µm long, the longest setae in mesial and submarginal regions, the shortest evenly distributed.

Trilocular pores. Each 3-4 µm in diameter ( Fig. 5B ), evenly distributed throughout.

Multilocular disc pores. Absent.

Oral rim tubular ducts. Absent.

Oral collar tubular ducts. Absent.

Discoidal pores. Of two sizes: I) shorter type, each 2-3 µm in diameter, similar diameter to or smaller than a trilocular pore ( Fig. 5C ), distributed from head to SabdVI; II) longer type, each 4-5 µm in diameter, wider than a trilocular pore ( Fig. 5D ) with border stout and sclerotized, distributed in SabdVII and SabdVIII + IX.

Spicules. Present from mesothorax to SabdVIII + IX.

Venter

Antennae. Each 391 ± 23.7 (351-435) µm long, 7 or 8 segmented [holotype with 7 segments] ( Fig. 5E ); all specimens seem to have at least one antennal segment atrophied, so segment length and chaetotaxy vary depending on number of segments: SantI 69.6 ± 5.7 (57-77) µm long, with four flagellate setae; SantII 55.1 ± 3.8 (49-61) µm long, with three to six flagellate setae and one placoid sensilla; SantIII 53.8 ± 6.0 (43-65) µm long, with one to five flagellate setae; SantIV 30.9 ± 9.7 (17-52) µm long, with one to two flagellate setae; SantV 41.2 ± 6.0 (29-57) µm long, with three to five flagellate setae; SantVI 44.0 ± 3.6 (38-53) µm long, with two to five flagellate setae and 0 or 1 fleshy seta; SantVII 57.0 ± 14.6 (46-101) µm long, with 3-16 flagellate and 0-2 fleshy setae; SantVIII 67.8 ± 17.3 (31-90) µm long, with 7-18 flagellate and 0-4 fleshy setae.

Eyespot. 35 ± 2.8 (29-39) µm in diameter; 0-3 associated discoidal pores ( Fig. 6C ).

Labium. 176 ± 32.1 [186] (92-198) µm long.

Fore leg. 533 ± 22.3 (504-569) µm long; trochanter + femur 268 ± 12.4 (250-291) µm long, femur 92 ± 8.4 (81- 103) wide; tibia + tarsus 221 ± 11 (207-234) µm long, tibia-tarsus union 42 ± 3.7 (36-48) µm wide; claw 44 ± 1.4 (42-47) µm long; chaetotaxy: coxa with nine flagellate setae, trochanter with seven, femur with 25, tibia internal margin of distal part with 6-13, tarsus with 11 setae and two tarsal digitules, the shortest 39 ± 4.8 (30-48) µm long, the longest 54 ± 2.6 (50-57) µm long; claw digitules each 36 ± 3.2 (29-39) µm long.

Mid leg. 563 ± 18.8 (527-591) µm long; trochanter + femur 283 ± 8.5 (270-304) µm long; femur 97 ± 5.6 (90-106) µm wide; tibia + tarsus 235 ± 10.9 (218-251) µm long, tibia-tarsus union 45 ± 3.5 (38-50) µm wide; claw 45 ± 2.5 (36-48) µm long; chaetotaxy: coxa with 11 flagellate setae, trochanter with seven to eight, femur with 13-25, tibia with 10-18 setae plus two spines (as fore tibia), tarsus with 11 setae and two unequal tarsal digitules, the shortest 43 ± 4.8 (33-50) µm long, the longest 55 ± 2.5 (50-57) µm.

Hind leg. 666 ± 27.6 (629-718) µm long, trochanter + femur 332 ± 16.4 (316-371) µm long; femur 134 ± 5.6 (125-142) µm wide; tibia + tarsus 287 ± 15.2 (259-306) µm long; tibia-tarsus union 50 ± 7.2 (39-64) µm wide; claw 47 ± 2 (43-50) µm long; femur with 398-460 [398-411] translucent pores and tibia with 111-154 [111-114] translucent pores ( Fig. 5F , 6D ); chaetotaxy: coxa with 11 flagellate setae, trochanter with seven, femur with 14-21, tibia with 13-21 setae plus two spines (as fore tibia), tarsus with 10 setae and two unequal tarsal digitules, the shortest 40 ± 5.9 (31-48) µm long, the longest 54 ± 3.1 (49-59) µm long ( Fig. 6E ); claw without a denticle, with two stout capitate ungual digitules each 36 ± 6.6 (24-44) µm long. Ratios: lengths of trochanter + femur /tibia + tarsus of fore, mid and hind legs 1.2 ± 0.1 (1.1-1.3); trochanter + femur length/femur width of fore and mid leg 2.9 ± 0.2 (2.6-3.2), and hind leg 2.5 ± 0.2 (2.2-2.9); tibia + tarsus length/tibia-tarsus union width fore and mid leg 5.3 ± 0.5 (4.3-6.2), and hind leg 5.8 ± 0.9 (4.4-7.8).

Circulus. Situated across intersegmental line between SabdIII and SabdIV ( Fig. 6F ), divided by intersegmental line, 130 ± 2.3 [128] (128-132) µm in transverse diameter.

Vulvar opening. Located between SabdVII and SabdVIII.

Anal lobes. Without anal bars, membranous and slightly protruding, each with 6-9 flagellate setae, apical seta 38 ± 8.5 (29-47) µm long ( Fig. 6G ).

Spicules. Present from metathorax to SabdVIII +IX.

Body setae. Flagellate, each 20 ± 5 (12-40) µm long, longest setae distributed in submesial and mesial areas, forming clusters in interantennal region, SabdVI to SabdVIII + IX and a few adjacent to cerarii; shortest setae evenly distributed over the entire surface.

Obanal setae. Each 18 ± 5.2 (12-24) µm long.

Cisanal setae. Each 32 ± 4.5 (25-36) µm long.

Multilocular disc pores. Each with 9 or 10 loculi ( Figs 5G ; 6H ) and 8 ± 2 (6-10) µm in diameter, distributed as follows: SabdIV [0] (0-1), SabdV [3] (8-16), SabdVI [20] (29-34), Sabd- VII [12] (31-39) and SabdVIII + IX [8] (10-16).

Oral rim tubular ducts. Absent.

Oral collar tubular ducts. Of two sizes: I) smaller type with diameter similar to a trilocular pore ( Fig. 5H , 6I ), each 3 ± 0.2 (2-3) µm in diameter and 6 ± 0.8 (6-8) µm long, forming transverse rows across SabdV [1] (1-17 pores), SabdVI [25] (25-47) and SabdVII [26] (26-37 pores); II) larger type each with a diameter greater than a trilocular pore ( Fig. 5I ), 5 ± 0.2 (5-6) µm in diameter and 8 ± 0.6 (6-9) µm long, distributed in clusters in marginal and submarginal regions of SabdIV [1] (1-20 ducts), SabdV [52] (52-79), SabdVI [47] (47-61) and SabdVII [32] (32-46 ducts).

Trilocular pores. Each 3-4 µm in diameter ( Fig. 5J ), evenly distributed.

Discoidal pores. Each narrower than a trilocular pore, 2-3 µm in diameter, evenly distributed ( Fig. 5K ).

REMARKS

There is a debate about the generic character state that defines Paraputo and Formicocccus. As Paraputo nasai n. sp. has six setae in the anal ring and no anal bar, there is no conflict to put it into the Paraputo genus. Paraputo nasai n. sp. comes closest to Paraputo colombiensis ( Williams & Granara de Willink, 1992) , another species with translucent pores on the hind femur and tibia and with a circulus present. Paraputo nasai n. sp. differs from P. colombiensis (character states in parenthesis) in having oral collar tubular ducts of two distinct sizes (oral collar ducts of one size only); multilocular disc pores on SabdIV and posterior segments (present on SabdVI and posterior segments); dorsal setae of uniform length over the entire surface (dorsal setae of two sizes, the shorter ones present from head to SabdIII, the longer ones present on SabdVI and posterior segments); number of conical cerarian setae progressively reduced anteriorly, from 6-12 setae in each of C 1 to C 6, to 2-3 setae in C 7 to C 18 (conical setae number constant amongst all the cerarii, with 4-6 setae per cerarius); and conical setae absent from marginal intercerarian spaces (conical setae present in marginal intercerarian spaces) [character data of P.colombiensis taken from Williams & Granara de Willink (1992)].

Figures

FIG. 5. — Taxonomic illustration of Paraputo nasai n. sp. adult ♀, with enlarged details: A, cerarii; B, trilocular pore; C, smaller discoidal pore; D, larger discoidal pore; E, antenna; F, hind femur and tibia; G, multilocular disc pore; H, smaller oral collar tubular duct; I, larger oral collar tubular duct; J, trilocular pore; K, discoidal pore. Scale bars: A, D, H-J, 5 µm; B, K, 3 µm; C, L, 2 µm; E, F, 100 µm.

FIG. 6. — Microphotographs of female specimens of Paraputo nasai Caballero n. sp.: A, body; B, cerarii; C, eyespot associated with discoidal pores; D, hind femur and hind tibia with translucent pores; E, tarsal digitules; F, circulus; G, anal lobes; H, multilocular disc pores on abdominal segment VII; I, large oral collar tubular ducts (one-tipped arrow) and small oral collar tubular duct (double-tipped arrow) on margin of abdominal segment V. Scale bars: A, 400 µm; B, 30 µm; C, E, G-I, 20 µm; D, 50 µm; F, 40 µm.