Niesslia guizhouensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Niesslia guizhouensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 26

Etymology.

In reference to Guizhou, the Province where the type specimen was obtained.

Type.

China: Guizhou Province, Guiyang City, North Campus of Guizhou University 26°44'37"N, 106°67'46"E, soil, 13 Sep 2019, Z.Y. Zhang (HMAS 351877 holotype designated here, ex-type living culture CGMCC 3.20780 = GZUIFR 21.912) ; ibid., GZUIFR 21.913 .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 26-27 mm diam., white (1A1), texture velvety, nearly round, margin entire; reverse white (1A1). Colony on MEA 14-16 mm diam., orange white (5A2), aerial mycelia sparse, compact, rugged, cracked, margin undulated; reverse brownish-yellow (5C8) to white (5A1) from centre to margin. Colony on OA 36 mm diam., white (1A1), with a light-coloured margin, felty, compact, plicated, convex, margin entire to undulate; reverse white (1A1).

Hyphae branched, septate, hyaline, smooth, 0.5-2.0 μm diam. Sporulation abundant, nematogenous to synnematogenous. Phialides from hyphae, hyphal coils, fertile, acerose at the moderately thick-walled base, sometimes bending, hardly widening above and tapering to 0.5-1 μm at the tip, 10.5-79.0 × 1.0-3.0 µm. Conidia adhering to slimy heads, cylindrical, smooth- and thin-walled, 3.0-7.5 × 1.0-2.5 μm (av. 5.3 × 1.9 μm, n = 50). Chlamydospores not observed. Sexual morph unknown.

Additional specimens examined.

China: Guizhou Province, Guiyang City, Qianlingshan Park 26°59'03"N, 106°69'57"E, soil, 13 Sep 2019, Z.Y. Zhang, GZUIFR 21.914 .

Notes.

Niesslia guizhouensis is phylogenetically related to N. ligustica , as demonstrated in Fig. 25 , but can be differentiated from it by the absence of pigmented cells ( Gams et al. 2019). In addition, the colony of N. guizhouensis on MEA are white with a white reverse, whereas those of N. ligustica are white or sometimes pale orange with a pale luteous to ochraceous reverse (Gams et al. in 2019).

Figures

Figure 25. Concatenated phylogeny of the ITS, LSU, EF 1 A and ACT gene regions of species in Niesslia. Sixty strains are used. The tree is rooted in Trichoderma aggressivum f. europaeum (CBS 100526). The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.7) and ML bootstrap values (≥ 70 %) and are indicated along branches (PP / ML). Novel species are in blue and bold font and " T " indicates type derived sequences.

Figure 26. Niesslia guizhouensis (from ex-holotype CGMCC 3.20780) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-j monocillium-like conidiophores and conidia k hyphal coil with conidiophores. Scale bars: 10 µm (d-k).