Lasiodiplodia pseudotheobromae A.J.L. Phillips et al.

Doilom, Mingkwan, Shuttleworth, Lucas A., Roux, Jolanda, Chukeatirote, Ekachai & Hyde, Kevin D., 2015, Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand, Phytotaxa 233 (1), pp. 1-26 : 19

Lasiodiplodia pseudotheobromae A.J.L. Phillips et al. , Fungal Diversity 28: 8 (2008)

Facesoffungi number: FoF00166 ( Figure 7 ).

Associated with trunk canker and branch dieback symptoms, and from dead twigs and branches of Tectona grandis . Sexual morph: Undetermined.Asexual morph: Conidiomata (330–) 360–385 (–450) μm high × 230–295 μm diam. (x

= 370 × 265 μm n = 10), pycnidial, solitary or scattered, dark brown to black, initially immersed, becoming erumpent,

uniloculate, globose or subglobose, with a central ostiole. Conidiomata wall 30–50 μm wide, outer layers dark brown to black, inner layers thin-walled, pale brown to hyaline, composed of 4–6 cell layers of textura angularis. Paraphyses up to 50 μm long, 1.5–3 μm wide, hyaline, mostly aseptate, cylindrical, ends rounded, numerous. Conidiogenous cells 6–12 ×3–4 μm (x = 8 × 3 μm n = 15), holoblastic, hyaline, cylindrical. Conidia on host (22–) 27–28.5 (–33) × 13–15 μm (x ± S.D. = 27 ± 2.7 × 14 ± 0.5 μm n = 20), initially hyaline and aseptate, becoming 1-septate at the centre, dark brown, thick-walled, ellipsoid to obovoid, guttulate, apex broadly rounded, base truncate or rounded, with longitudinal striations from apex to base.

Culture characteristics: —Conidia germinating on PDA after 5 h. Germ tubes produced from both lateral ends of the ascospore. Colonies on MEA reaching 40 mm diam after 1 day in the dark at 25 °C, cotton-like, fast growing,

raised, fluffy, undulate, dense, filamentous, initially white, after 1 week becoming grey (4F1) at the edge, white in the centre, reaching the edge of the Petri-dish after 2 days.

Material examined: — THAILAND, Phayao Province, Chun District, Hong Hin Sub-district, on twigs of T.

grandis with dieback symptoms, 23 November 2012, M. Doilom and J. Roux (living culture MFLUCC 12-0772, MUCL

55410); Phayao Province, Muang District , on dead branch of T. grandis , 12 March 2012, Phongeun Sysouphanthong ,

(living culture MFLUCC 12-0294); Phayao Province, Muang District , on dead branch of T. grandis , 12 March 2012 ,

M. Doilom (living culture MFLUCC 12-0295); Chiang Rai Province, Muang District, San Sai Sub-district, Pong Sa

Lee Arboretum, from basal trunk canker symptoms of T. grandis , 2 December 2012, M. Doilom and K. Jatuwong

(living culture MFLUCC 12-0796, MUCL 55411); Phrae Province, Song District, Ban Rainadeaw Sub-district , on dead twig of T. grandis , 30 December 2011, M. Doilom ( MFLU 14-0270 , living culture MFLUCC 12-0053 , MUCL

55407).

Notes: —Conidia and paraphyses of L. pseudotheobromae ( MFLU 14-0270) were shorter and smaller than in the holotype. The variation may be related to different hosts. The type was collected from Gmelina arborea . Our collection of Lasiodiplodia pseudotheobromae on Tectona grandis is illustrated and described here to amend the previous descriptions of L. pseudotheobromae .

Figures

FIGURE 7. Lasiodiplodia pseudotheobromae (MFLU 14-0270) a, b. Conidiomata and conidia on surface of dead twig of Tectona grandis. c. Section through conidioma. d. Conidioma wall. e. Conidia attached to conidiogenous cells with paraphyses. f, g. Immature conidia. h–j. Mature conidia in two different focal planes showing longitudinal striations. k, l. Germinated mature conidia. Note e, g stained with lacto-phenol cotton blue. Scale bars: a=300 μm. b=200 μm. c=100 μm. d, f=20 μm. e, g–l=10 μm.