Lasiodiplodia brasiliense M.S.B. Netto et al.

Doilom, Mingkwan, Shuttleworth, Lucas A., Roux, Jolanda, Chukeatirote, Ekachai & Hyde, Kevin D., 2015, Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand, Phytotaxa 233 (1), pp. 1-26 : 19

Lasiodiplodia brasiliense M.S.B. Netto et al. in Netto et al., Fungal Diversity 67: 134 (2014)

Facesoffungi number: FoF 00628 ( Figure 6 ).

Saprobic on dead branch of Tectona grandis . Sexual morph: Undetermined. Asexual morph: Conidiomata pycnidial formed on MEA after 1 month, dark brown, erumpent. Paraphyses up to 60 μm long, 1.5–5.5 μm wide, hyaline, septate, cylindrical, ends rounded, numerous. Conidiogenous cells 6–15 × 1.5–6 μm (x = 10 × 3.5 μm n = 20), holoblastic, hyaline, cylindrical. Conidia on host (22–) 26–27 (–29) × 12–16 μm (x ± S.D. = 26 ± 1.6 × 14 ± 1 μm n = 30), in culture (19–) 25–27 (–28) × 12–17 μm (x ± S.D. = 25 ± 2 × 15 ± 1 μm n = 30), initially hyaline and aseptate,

becoming 1-septate, dark brown, thick-walled, ellipsoid to obovoid, guttulate, apex broadly rounded, base truncate or round, with longitudinal striations from apex to base.

Culture characteristics: —Conidia germinating on PDA after 5 h. Germ tubes produced from both ends of conidia.

Colonies on MEA reaching 50 mm diameter after 1 day in the dark at 25 °C, fast growing, raised, fluffy, undulate,

dense, filamentous, convex with papillate surface, initially white, after 2 days becoming pale grey and becoming dark grey (1F1) after 1 week, reaching the edge of the Petri-dish after 2 days.

Material examined: — THAILAND, Chiang Rai Province, Mae Fah Luang District, on dead branches of Tectona grandis , 4 May 2011, M. Doilom (living culture MFLUCC 11-0414, MUCL 55406).

Notes: — Lasiodiplodia brasiliense collected in this study differs from the type species in having septate paraphyses, although this may have been overlooked in the type ( Netto et al. 2014). They also differ in hosts. In this study L. brasiliense was collected from T. grandis , while the type was collected from Mangifera indica . Our collection from Tectona grandis is illustrated and described here to facilitate identification from this host.

Figures

FIGURE 6. Lasiodiplodia brasiliense (MFLUCC 11-0414) a. Conidia formed on dead branches of Tectona grandis after incubation in a moist chamber for10 days. b. Colony on MEA after 1 week, and inset a conidioma on the agar surface after 1 month. c. Immature conidia attached to conidiogenous cells with paraphyses. d-f. Immature conidia attached to conidiogenous cells. g. Paraphyses. h. Immature conidia. i–k. Mature conidia. Note: c stained with lactophenol cotton blue. c–i, k. Morphology in culture. j morphology on host. Scale bars: a=100 μm. b=300 μm. c–k=10 μm.