Diaporthe subclavata F. Huang, K.D. Hyde & H.Y. Li, Fung. Biol. 119: 343, 2015

Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang, 2021, Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species, MycoKeys 77, pp. 65-95 : 65

Diaporthe subclavata F. Huang, K.D. Hyde & H.Y. Li, Fung. Biol. 119: 343, 2015 Figure 10

Description.

Asexual morph: Conidiomata pycnidial, multi-pycnidia grouped together, globose, black, erumpent, coated with white hyphae, creamy to yellowish conidial droplets exuded from central ostioles. Conidiophores hyaline, densely aggregated, cylindrical, straight to sinuous, tapering towards the apex, 13.5-23.0 × 2.0-3.0 μm. Alpha conidiogenous cells 7.0-10 × 1.8-2.5 μm, cylindrical, terminal, slightly curved. Beta conidiogenous cells 10.5-13.5 × 0.9-1.5 μm, cylindrical, hyaline, tapering towards the apex. Alpha conidia, hyaline, smooth, aseptate, ellipsoidal, multi-guttulate, apex subobtuse, base subtruncate, 4.7-5.8 × 2.4-2.9 µm (mean = 5.3 × 2.6 μm, n = 20). Beta conidia hyaline, aseptate, filiform, few guttulate, slightly curved, tapering towards the both ends, 25.5-32.0 × 1.0-1.6 µm (mean = 27.5 × 1.3 μm, n = 20). Gamma conidia and sexual morph not observed.

Culture characteristics.

Pure culture was isolated by subbing hyphal tips growing from surface sterilized diseased material. Colonies on PDA cover the Petri dish diameter after incubation for 15 days in dark conditions at 25 °C. Aerial mycelium white, cottony, feathery, with concentric zonation, white on surface side, pale brown to black on reverse side.

Specimen examined.

China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on infected leaves of Pometia pinnata . 19 April 2019, S.T. Huang, HSAUP194.66, living culture SAUCC194.66.

Notes.

Diaporthe subclavata was originally described from the leaf with citrus scab of Citrus unshiu in Fujian Province, China ( Huang et al. 2015). In the present study, isolated strain SAUCC194.66 from symptomatic leaves of Pometia pinnata was congruent with D. subclavata based on morphology and DNA sequences data (Fig. 1 ). We therefore present a description and illustration of D. subclavata as a known species for this clade, found on new host.

Figures

Figure 1. Phylogram of Diaporthe based on combined ITS, TUB, TEF, CAL and HIS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Three branches were shortened to fit the page size - these are indicated by symbol (//) with indication number showing how many times they are shortened.

Figure 10. Diaporthe subclavata (SAUCC 194.66) a leaf of Pometia pinnata b, c surface (b) and reverse (c) sides of colony after incubation for 15 days on PDA d conidiomata e-h conidiophores and conidiogenous cells i, j Beta conidia k, l Alpha conidia. Scale bars: 10 μm (e-l).