Torula canangae N.I. de Silva,S, Lumyong & K.D. Hyde. Mycosphere 13(1): 955-1076 (2022)

Wang, Wen-Peng, Shen, Hong-Wei, Bao, Dan-Feng, Lu, Yong-Zhong, Yang, Qiu-Xia, Su, Xi-Jun & Luo, Zong-Long, 2023, Two novel species and three new records of Torulaceae from Yunnan Province, China, MycoKeys 99, pp. 1-24 : 1

Torula canangae N.I. de Silva,S, Lumyong & K.D. Hyde. Mycosphere 13(1): 955-1076 (2022)

Fig. 3

Description.

Saprobic on submerged decaying wood. Sexual morph: Undetermined. Asexual morph: Colonies effuse on the natural substrate, neat, hairy, brown. Mycelium immersed to superficial, hyaline, septate, branched hyphae. Conidiophores indistinct. Conidiogenous cells 6-10(-13) × 5-7 (-13) μm (x̄ = 8 × 7 μm, n = 15), holoblastic, mono-to polyblastic, integrated, terminal, doliiform to spherical, brown to dark brown. Conidia (28-) 78-113 (-142) × 6-9 μm (x̄ = 82 × 7 μm, n = 20), acrogenous, dry, brown to dark brown, subhyaline at terminal cell, constricted at septa, verrucose, easily separating, 5-29-septate, cell size is uniform, chiefly subcylindrical.

Culture characters.

Conidia germinating on PDA within 12 h, and germ tubes produced at the side. Mycelium superficial, branched, septate, hyaline, smooth. After two weeks of incubation at room temperature, colony appears distinctly rounded, the central hyphae are longer, white, velvety, and the edges are white to brown and the hyphae are shorter.

Material examined.

China, Yunnan Province, Wenshan, Bamei Town (24°31′96.49″N, 105°03′84.35″E), on submerged decaying wood, 7 February 2022, Wen-Peng Wang S 3492 (KUN-HKAS 124619), living culture, KUNCC 22-12432 = CGMCC 3.24258 .

Notes.

Silva et al. (2022) first introduced Torula canangae , which was collected from terrestrial habitats on dead twigs of Cananga odorata in Thailand. In this study, phylogenetic analyses showed that our collection clustered with the ex-type strain of T. canangae (MFLUCC 21-0169) with 100% ML/1.00 PP support (Fig. 1 ). Our collection has similar morphological features to T. canangae , such as indistinct conidiophores, ellipsoid to coronal, terminal conidiogenous cells, and mainly subcylindrical conidia ( Silva et al. 2022). Thus, we identify our isolate as T. canangae based on both morphology and multigene phylogeny, and it is a new record for freshwater habitat in China.

Figures

Figure 1. The maximum likelihood (ML) tree based on combined LSU, ITS, SSU, tef 1 - α, and rpb 2 sequence data. Bootstrap support values with an ML greater than 75 % and Bayesian posterior probabilities (PP) greater than 0.95 are given above the nodes, shown as " ML / PP ". The tree is rooted with Occultibambusa bambusae (MFLUCC 13 - 0855) and Neooccultibusa thailandensis (MFLUCC 16 - 0274). New species and collections are indicated in red; while the type strains are in bold black.

Figure 3. Torula canangae (KUNCC 22 - 12432) A fungal structure on the substratum B-D conidiogenous cells E conidiogenous cells with conidia F-K conidia L germinating conidium M, N colonies on PDA from surface and reverse. Scale bars: 10 μm (B-D), 15 μm (E), 30 μm (F-H), 50 μm (I-K), 100 μm (L).