Nothophoma naiawu E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan, 2021

Keirnan, Elizabeth C., Tan, Yu Pei, Laurence, Matthew H., Mertin, Allison A., Liew, Edward C. Y., Summerell, Brett A. & Shivas, Roger G., 2021, Cryptic diversity found in Didymellaceae from Australian native legumes, MycoKeys 78, pp. 1-20 : 1

Nothophoma naiawu E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan sp. nov. Fig. 5

Type.

Australia, South Australia, Blanchetown, from Senna artemisioides , 22 Oct. 2016, E.C. Keirnan, holotype BRIP 69583 (includes culture ex-type).

Description.

Colonies on OA, 21-25 mm diam. after 7 d, flat with scant aerial mycelia, rosy vinaceous, dark at centre; reverse rosy buff, dark at centre, with a few dark radiating fissures; on MEA, 27-30 mm after 7 d, margin entire, flat, with sparse aerial mycelium towards centre rosy vinaceous; reverse peach, darker at centre; on PDA, 27-30 mm after 7 d, margin entire, flat felty, rosy buff; reverse peach, dark at centre. NaOH spot test: slightly yellow. Conidiomata pycnidial, globose to subglobose, 200-300 μm diam., pale brown becoming black, semi-immersed, confluent on MEA, glabrous, non-papillate; ostiole c. 25 μm diam.; pycnidial wall composed of textura globulosa, pale brown, cells 5-8 μm diam.. Conidiogenous cells phialidic, cylindrical, very thin-walled, hyaline. Conidia aseptate or 1-septate, 8-12 × 4-6 μm, cylindrical to narrow ellipsoidal, pale yellow.

Etymology.

A variation of the Indigenous Australian Ngayawang people’s language group, who lived in the Murray River region of South Australia, which includes Blanchetown, the locality where this specimen was collected.

Notes.

Nothophoma naiawu is phylogenetically close to No. eucalyptigena and No. infuscata (Fig. 2 ). Nothophoma naiawu is easily distinguished from No. eucalyptigena and No. infuscata by the ITS region (98 % identity to both) and the rpb 2 locus (95%, and 94% identity, respectively). Nothophoma infuscata produce a pale red discolouration in response to NaOH spot test on MEA media, which is distinct from the slightly yellow response by No. naiawu .

Figures

Figure 2. Epicoccum djirangnandiri: a leaf lesions on Swainsona galegifolia b 14 - d old colonies on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e conidia. Scale bars: 200 µm (d); 7 µm (e).

Figure 5. Nothophoma naiawu: a pin-prick leaf spots on Senna artemisioides b 14 - d old colonies top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e pycnidia f conidia. Scale bars: 300 µm (d, e); 10 µm (f).