Doryphoribius barbarae, Beasley, Clark W. & Miller, William R., 2012

Beasley, Clark W. & Miller, William R., 2012, Additional Tardigrada from Hubei Province, China, with the description of Doryphoribius barbarae sp. nov. (Eutardigrada: Parachela: Hypsibiidae), Zootaxa 3170, pp. 55-63 : 58-61

Doryphoribius barbarae sp. nov.

FIGURES 2 A–D, TABLE 1

Material examined. Holotype (slide No. 13-303-9) and three paratypes (13-303-18 (simplex), 13-303-21, 13-303- 22), three exuvia with eggs (13-303-14, 13-303-15, 13-340-2); moss sample from Hubei Province, China, Tiansheng Bridge area, Site 5, 1356 m asl, 15 June 2005; collected by C. Beasley.

Specific diagnosis. Doryphoribius with two macroplacoids, no microplacoid. Cuticle with irregularly shaped and arranged tubercles. Body with nine rows of gibbosities (IX: 2-4-4-4-4-6-4-4-2).

Description of holotype. Body length 221.1 µm. Body colour unknown, no eyes observed. Nine rows of gibbosities (IX: 2-4-4-4-4-6-4-4-2) on dorsal and lateral surfaces of the body ( Fig. 2 A) Dorsal gibbosities at the level of the third legs 16.9 µm in diameter. Dorsal and lateral surfaces of body, including gibbosities, with irregularly shaped and arranged tubercles ( Fig. 2 C), dimensions mostly ranging from 0.5 to 3.2 µm. Claws of the Isohypsibius type, similar in shape and size ( Fig. 2 B, Table 1 ), primary branches with small accessory points. No lunules or cuticular bars on legs.

Anterio-ventral mouth. Bucco-pharyngeal apparatus of the Doryphoribius type, with peribuccal lamellae and papulae absent, oral cavity armature not seen. Buccal tube slightly curved in anterior half, 28.9 µm long, 2.7 µm (pt = 9.3) external diameter. Ventral lamina 13.6 µm (pt = 47.1) long ( Fig. 2 D). Pharyngeal apophyses at the end of the buccal tube of slender, triangular shape. The insertion of the stylet supports on the buccal tube at 19.9 µm (pt = 68.9). Pharynx with two elongated macroplacoids ( Fig. 2 D). The first 5.0 µm (pt = 17.3) long, slightly curved, with slight constriction a little anterior of its middle. The second macroplacoid 3.6 µm (pt = 12.5) long, slightly curved, with slight sub-terminal constriction. Macroplacoid row length 9.3 µm (pt = 32.2). As in all described species of Doryphoribius , no microplacoid or septulum.

Remarks. The two anterior rows of gibbosities were not clearly visible in all specimens. When visible, the gibbosities of the first row were not as proportionally high as in the more posterior rows.

Three exuvia contained smooth, oval eggs (3, 3, 1).

Etymology. The species is named after Barbara, the wife of the first author.

Type depositories. The holotype, an exuvium with eggs, and one other adult (slide numbers, respectively, 13- 303-9, 13-303-15, and 13-303-24 (final catalogue numbers not available)) are deposited in the Tardigrada collection at the College of Life Sciences, Shaanxi Normal University, Xian, China. A paratype and an exuvium with eggs are deposited at each of the following locations: the Binda and Pilato collection (Museum of the Department of Animal Biology “Marcello La Greca”, University of Catania, Italy): slide numbers 5460 (paratype, 13-303-22) and 5461 (exuvium with eggs, 13-340-2) and the Academy of Natural Sciences, Philadelphia, Pennsylvania, U.S.A.: slide numbers ANSP-C000700 (paratype, 13-303-21), ANSP-C000701 (13-303-18), ANSP-C000702 (13- 303-10), ANSP-C000703 (13-391-1), ANSP-C000704 (13-391-9).

Differential diagnosis. Michalczyk & Kaczmarek (2010) divided the Doryphoribius species into four groups using the number of macroplacoids and the presence or absence of cuticular gibbosities. Using their criteria, D. barbarae sp. nov. fitted into the evelinae group. Michalczyk & Kaczmarek (2010) named nine species in this group, and since then D. tesselatus Meyer, 2011 , has been added ( Meyer 2011). Lisi (2011) stated that his new species D. amazzonicus Lisi, 2011 “should belong to the evelinae group” but expressed doubts concerning the value of the groups; he does not describe dorsal gibbosities and compares D. amazzonicus to species without dorsal gibbosities. Although not specifically stated, his new species apparently lacks dorsal gibbosities, but he does describe swellings on the legs.

D. barbarae sp. nov. differed from the existing species within the evelinae group in the following ways: D. tessellatus Meyer, 2011 , had only three rows of gibbosities, compared to the nine of D. barbarae sp. nov. D. evelinae ( Marcus 1928) , D. flavus ( Iharos 1966) , D. amazzonicus Lisi, 2011 , and D. dawkinsi Michalczyk & Kaczmarek, 2010 , were described with gibbosities on at least some of the legs, which was lacking in D. barbarae sp. nov.

D. picoensis Fontoura et al., 2008 , lacked cuticular tubercles which were present on D. barbarae sp. nov.

Some of the evelinae group species exhibit a different arrangement of gibbosities than D. barbarae sp. nov. (IX: 2-4-4-4-4-6-4-4-2): D. dupliglobulatus Ito, 1995 , seven rows of two each; D. huangguoshuensis Wang et al. 2007 , IX: 4-4-4-4-4-4-4-2-2; D. maranguensis Binda & Pilato 1995 , only indistinct gibbosities plus approximately 20 transverse undulations; D. quadrituberculatus Kaczmarek & Michalczyk 2004 , eleven transverse undulations plus two rows of two gibbosities on the caudal end; D. zyxiglobus ( Horning et al. 1978) , VIII: 6-4-6-4-6-4-4-2.

D. maranguensis was described with indistinct gibbosities and irregularly shaped tubercles, so it could be mistaken for D. barbarae sp. nov., but the macroplacoids are very different: in a D. maranguensis specimen of 331 µm, the first macroplacoid is 9.98 µm (pt = 25.3), the second 5.9 µm (pt = 14.9) ( Binda & Pilato 1995); in a D. barbarae sp. nov. specimen of 294 µm, the first macroplacoid is 4.1 µm (pt = 13.1), the second 3.1 µm (pt = 9.9).

Astatumen bartosi (W ę glarska, 1959)

Material examined. One specimen was found in a moss sample from Site 4.

Remarks. This specimen fitted the description, including quantitative values, given in Ramazzotti and Maucci (1983) and Dastych (1988), except that the salivary glands were not visible. This is a new record for China.

Figures

FIGURE 2. Doryphoribius barbarae sp. nov. A, habitus with some gibbosities visible; B, claws of leg IV; C, cuticular pattern of dorsum between second and third legs; D, buccal tube and placoids, ventral lamina visible. Scale line A, 50 µm; other scale lines 10 µm.

Tables

TABLE 1. Selected morphometric characters of Doryphoribius barbarae sp. nov. Abbreviations: ED, external diameter; ext, external; int, internal; ant, anterior; post, posterior; bc, basal claw; pb, primary branch; sb, secondary branch.

	Holotype	Paratype 1	Paratype 2
Body length	µm pt 221.1 -	µm pt 235.4 -	µm pt 239.6 -
Buccal tube length	28.9 -	29.2 -	30.2 -
Stylet supports insertion Buccal tube ED	19.9 68.9 2.7 9.3	22.1 75.7 3.4 11.6	22.9 75.8 3.3 10.9
Ventral lamina Macroplacoid 1	13.6 47.1 5.0 17.3	20.5 70.2 4.8 16.4	19.4 64.2 5.1 16.9
Macroplacoid 2	3.6 12.5	3.5 12.0	3.1 10.3
Macroplacoid row Claw I, bc (ext) Claw I, pb (ext) Claw I, sb (ext) Claw I, bc (int)	9.3 32.2????????	8.8 30.1 3.9 13.4 8.5 29.1 7.0 24.0 2.4 8.2	8.8 29.1????????
Claw I, pb (int) Claw I, sb (int)	????	6.5 22.3 5.6 19.2	????
Claw II, bc (ext) Claw II, pb (ext) Claw II, sb (ext) Claw II, bc (int) Claw II, pb (int)	4.1 14.2 8.4 29.1 6.2 21.5 3.3 11.4 7.7 26.6	3.3 11.3 9.6 32.9 5.8 19.9?? 7.4 25.3	4.7 15.6 10.2 33.8 7.3 24.2 3.5 11.6 8.6 28.5
Claw II, sb (int) Claw III, bc (ext) Claw III, pb (ext) Claw III, sb (ext) Claw III, bc (int)	5.6 19.4 4.1 14.2?? 6.2 21.5 2.9 10.0	6.0 20.5 2.9 9.9 10.3 35.3 6.5 22.3 2.2 7.5	5.6 18.5 5.5 18.2 10.1 33.4 7.1 23.5 3.5 11.6
Claw III, pb (int) Claw III, sb (int)	8.3 28.7 5.7 19.7	8.3 28.4 5.6 19.2	8.4 27.8 5.8 19.2
Claw IV, bc (ext/ant) Claw IV, pb (ext/ant) Claw IV, sb (ext/ant) Claw IV, bc (int/post) Claw IV, pb (int/post)	3.9 13.5 8.0 27.7 5.8 20.1 4.9 17.0 8.3 28.7	??????????	4.4 14.6 8.7 28.8 6.2 20.5 5.1 16.9 9.6 31.8
Claw IV, sb (int/post)	6.0 20.8	??	7.2 23.8