Epicoccum djirangnandiri E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan, 2021

Keirnan, Elizabeth C., Tan, Yu Pei, Laurence, Matthew H., Mertin, Allison A., Liew, Edward C. Y., Summerell, Brett A. & Shivas, Roger G., 2021, Cryptic diversity found in Didymellaceae from Australian native legumes, MycoKeys 78, pp. 1-20 : 1

Epicoccum djirangnandiri E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan sp. nov. 833689 Fig. 2

Type.

Australia, New South Wales, Mount Annan, Swainsona galegifolia , 19 Jan. 2017, E.C. Keirnan (holotype BRIP 69585, includes culture ex-type).

Description.

Colonies on OA, 76-80 mm diam. after 7 d, covered in dense aerial mycelium, variable shades of grey, pale cinnamon towards centre; reverse dark vinaceous; on MEA, 70-72 mm after 7 d, margin entire, covered in low dense aerial mycelium, pale mouse grey with lighter patches; reverse olivaceous with radiating spokes; on PDA, 73-80 mm after 7 d, margin entire, mycelia felty, mouse grey becoming vinaceous buff towards centre; reverse fuscous black. NaOH spot test: negative. Conidiomata on CLA, pycnidial, globose 100-200 μm diam., pale brown becoming black, solitary, glabrous, non-papillate; pycnidial wall composed of textura globulosa, pale brown, cells 5-15 μm diam. Conidiogenous cells phialidic, cylindral, thin-walled, hyaline, rounded ends. Conidia aseptate, 5-7 × 2-3 μm.

Etymology.

From the language of the Indigenous Australian Dharawal people, meaning leaf spot. The Dharawal people are from the western Sydney region in New South Wales, which includes Mount Annan, where the holotype was collected.

Notes.

Epicoccum djirangnandiri is phylogenetically close to E. pneumoniae ex-type strain UTHSC DI16-257 (Fig. 1 ) and is distinguished in rpb 2 sequences with 99% identity. Morphological comparisons could not be made as E. pneumoniae was sterile in culture ( Valenzuela-Lopez et al. 2018). Epicoccum djirangnandiri is only known from one specimen on Swainsona galegifolia .

Figures

Figure 1. Phylogenetic tree based on maximum likelihood analysis of the combined multilocus (rpb 2 and ITS) alignment. RAxML bootstrap values (bs) greater than 70 % and Bayesian posterior probabilities (pp) greater than 0.95 are given at the nodes (bs / pp). Genera are delimited in coloured boxes, with the genus name indicated to the right. Isolates identified in this study are in bold, and novel taxa are in red bold. Ex-type isolates are marked with T. The outgroup is Neoascochyta desmazieri (CBS 297.69).

Figure 2. Epicoccum djirangnandiri: a leaf lesions on Swainsona galegifolia b 14 - d old colonies on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e conidia. Scale bars: 200 µm (d); 7 µm (e).