Rosellinia lamprostoma Syd. & P. Syd., Philipp. J. Sci., C, Bot.

Long, Sihan, Pi, Yinhui, Wu, Youpeng, Liu, Lili, Zhang, Xu, Long, Qingde, Lin, Yan, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N. & Li, Qirui, 2022, Rosellinia qiongensis sp. nov., R. verticillata sp. nov. and a new record of R. lamprostoma from China, Phytotaxa 552 (5), pp. 287-300 : 293

Rosellinia lamprostoma Syd. & P. Syd., Philipp. J. Sci., C, Bot. 8(4): 273 (1913) ( Fig. 2 )

MycoBank No: MB 159461

Saprobic on dead twig of unidentified plant. Sexual morph Subiculum felted, reddish brown, restricted to rim around stromata, evanescent. Stromata 750–1100 µm high × 700–960 µm diam. (av. = 943 × 808 µm, n = 20), superficial, conical, pear shaped, scattered to gregarious, solitary, conical, dark brown to black, shiny, carbonaceous. Ostioles black, finely to coarsely papillate. Ectostroma 100–120 µm thick, black, carbonaceous. Entostroma disappearing at maturity. Perithecia nearly semiglobose, not collapsed. Asci 246–270 × 16–22 µm (av. = 258.9 × 19.5 µm, n = 30), 8-spored, cylindrical, short pedicellate, apically rounded, with a J+ apical apparatus in Melzer’s reagent, urn-shaped, 14–18.5 µm high, 5.5–8 µm wide. Ascospores 39–54 × 9–11 µm (av. = 46 × 10 µm, n = 30), overlapping uniseriate, fusiform, rounded ends, hyaline when immature, slight brown to brown at maturity, straight to curved, with a germ slit running the entire length of the spores, slightly constricted at the middle part, lacking slimy sheathes or appendages. Asexual morph Undetermined.

Culture characteristics: —Ascospores germinated on PDA within 1 month. Colonies on PDA were white when young, became pale brown, dense, but thinning towards the edge, medium dense, white from above, reverse side white at the margin, flesh to pale brown at centre. No pigmentation was produced on the PDA medium. No conidia were observed on PDA or OA media.

Habitat/Distribution: —Known to inhabit dead bamboo, Taiwan province, China, Luzon, Philippine Island.

Material examined: — CHINA, Hainan Province, Wuzhishan, Emerald Park (18°47′11.85″N, 109°31′0.13″E), altitude: 360 m, dead branch of unidentified plant, 15 November 2020, S.H. Long, FCGY14 ( GMB0086 , KUN-HKAS 122643 , living culture GMBC0086 ) (New geographical record) .

Addition sequences: — LSU: OM 001209

Notes: —In our phylogenetic analyses ( Figure 1 ), new collection ( GMB 0086) accommodated with R. lamprostoma ( YMJ 89112602) and R. mearnsii ( MFLU 16-1382). However, GMB 0086 morphologically resembles R. lamprostoma . A morphological comparison of the new collection with these two new species is provided in Table 2 . Stromata are conical, pear-shaped in GMB 0086 and R. lamprostoma , which is different from globose stromata in R. mearnsii . And the stromata size of GMB 0086 is close to R. lamprostoma but larger than R. mearnsii . The size of the ascospores is closer to R. lamprostoma but wider than R. mearnsii ( Petrini 2013, Tibpromma et al. 2017). Moreover, GMB 0086 have the spore-lenght germ slits which are similar to R. lamprostoma ( Petrini 2013) . Therefore, we identify GMB 0086 as R. lamprostoma . Rosellinia lamprostoma has been reported in Philippine Islands, and Taiwan ( Ju et al. 2007, Petrini 2013). This is the first report of R. lamprostoma in Chinese mainland.

Figures

FIGURE 1. Phylogram generated from Maximum Likelihood (RAxML) analyses, based on ITS-β-tubulin-α-actin matrix. ML bootstrap supports (≥ 75%) and Bayesian posterior probability (≥ 0.95) are indicated as ML/BYPP. The tree is rooted to Amphilogia gyrosa (YMJ 91123101). Type strains are in bold and newly generated strains are in red bold.

FIGURE 2. Rosellinia lamprostoma (GMB0086) A. Host. B. Stromata on host substrate C. Close-up of stroma D. Transverse section trough stroma E. Vertical section through stroma F–H. Asci I. Ascus apical ring in Melzer’s reagent. J, K. Ascospores L. Ascospores in black ink M. Culture on PDA. Scale bars: B = 1 mm, C–E = 200 μm, F–L = 10 μm.