Neoroussoella entadae Jayasiri, E. B. G. Jones & K. D. Hyde

Htet, Zin Hnin, Hyde, Kevin D., Alotibi, Fatimah O., Chethana, Thilini K. W. & Mapook, Ausana, 2024, Multigene phylogeny, taxonomy, and potential biological properties of Pseudoroussoella and Neoroussoella species (Roussoellaceae, Dothideomycetes) from Asteraceae weeds in northern Thailand, MycoKeys 111, pp. 129-146 : 129-146

publication ID

https://doi.org/ 10.3897/mycokeys.111.136922

DOI

https://doi.org/10.5281/zenodo.14510046

persistent identifier

https://treatment.plazi.org/id/1CAEDF93-E94B-5388-B4E3-D88B954D009B

treatment provided by

MycoKeys by Pensoft

scientific name

Neoroussoella entadae Jayasiri, E. B. G. Jones & K. D. Hyde
status

 

Neoroussoella entadae Jayasiri, E. B. G. Jones & K. D. Hyde View in CoL , Mycosphere 10 (1): 105 (2019)

Fig. 3 View Figure 3

Description.

Saprobic on the dead stems of Chromolaena odorata ( Asteraceae ). Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 70–120 × 100–150 µm (av. 95 × 110 µm, n = 5), pycnidial, solitary, globose to subglobose, uniloculate, immersed to semi-immersed, ostiolate. Ostiole 30–35 µm wide, with small papillate. Peridium 10–20 µm wide, comprising 2–3 layers of brown cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 3–5 × 1–3 µm (av. 3.8 × 2.1 µm, n = 10), phialidic, ampulliform to cylindrical, hyaline. Conidia 3–5 × 2–4 μm (av. 3.8 × 2 μm, n = 20), hyaline, becoming pale brown when gathering, oblong to ovoid, aseptate, with small guttules.

Culture characteristics.

Conidia germinating on PDA within 24 hours, reaching 24 mm after 7 days at 27 ° C, irregular, entire, yellowish-brown, slightly wrinkled on the surface; pale brown to brown in reverse.

Material examined.

Thailand • Chiang Rai Province, Thoeng district , on dead stems of Chromolaena odorata ( Asteraceae ), 24 Jan 2022, A. Mapook (TCR 18, MFLU 24-0265 , new host record); living culture MFLUCC 24-0275 .

Known host distribution.

Entada phaseoloides ( Fabaceae ), Leucaena sp. ( Fabaceae ) ( Jayasiri et al. 2019).

Notes.

In a BLASTn search of GenBank, the closest match for the ITS sequence of our isolate was N. solani strain MnF 107 (OQ 704272) with 99.83 % similarity. The closest match for the LSU region was Roussoella sp. strain HF 3 S 53 ( OP 179275 View Materials ) with 99.77 % similarity, and the closest match for the SSU region was Pleosporales sp. IRB 20-2 (AB 195632) with 100 % similarity. The closest match for the tef 1 - α and rpb 2 gene region was Neoroussoella entadae strain MFLUCC 18-0243 ( MK 434866 View Materials ) with 99.78 % and 99.53 % similarity, respectively.

When we compared the morphology, our isolate is similar to N. entadae ( MFLUCC 17–0920 ) in having solitary, unilocular, ostiolate conidiomata, phialidic, ampulliform to cylindrical, hyaline conidiogenous cells, and oblong to ovoid, hyaline conidia with size (3–5 × 2–4 μm vs 3–4 × 1.7–1.9 μm). However, our isolate differs from N. entadae ( MFLUCC 17–0920 ) in having smaller conidiomata (70–120 × 100–150 µm vs. 127–192 × 161–190 µm), slightly wider conidiogenous cells (3–5 × 1–3 µm vs. 3.5–5.6 × 0.7–1.8 µm) (Table 3 View Table 3 ).

Based on the multi-locus phylogeny (Fig. 1 View Figure 1 ), our isolate MFLUCC 24-0275 clustered in the same clade with N. entadae ( MFLUCC 18-0243 ). Moreover, there is no significant base pair difference between MFLUCC 24-0275 and N. entadae ( MFLUCC 18-0243 ). Therefore, we reported N. entadae as the first occurrence on C. odorata ( Asteraceae ) based on morphology and multigene phylogeny.

Kingdom

Fungi

Phylum

Ascomycota

Class

Dothideomycetes

Order

Pleosporales

Family

Roussoellaceae

Genus

Neoroussoella

Loc

Neoroussoella entadae Jayasiri, E. B. G. Jones & K. D. Hyde

Htet, Zin Hnin, Hyde, Kevin D., Alotibi, Fatimah O., Chethana, Thilini K. W. & Mapook, Ausana 2024
2024
Loc

Neoroussoella entadae

Jayasiri, E. B. G. Jones & K. D. Hyde 2019: 105
2019