Cicurina (Cicurella) madla Gertsch, 1992

Hedin, Marshal, Derkarabetian, Shahan, Blair, Jennifer & Paquin, Pierre, 2018, Sequence capture phylogenomics of eyeless Cicurina spiders from Texas caves, with emphasis on US federally-endangered species from Bexar County (Araneae, Hahniidae), ZooKeys 769, pp. 49-76 : 58

Cicurina (Cicurella) madla Gertsch, 1992 Figs 1 , 2 , 3 , 4 , 5 , 6 , 10 , 11 , 12 , Suppl. materials 1, 2, 3, 5, 6.

Cicurina madla Gertsch, 1992: 109, figs 91-92.

Cicurina madla Cokendolpher, 2004: 42, figs 7, 40-47.

Cicurina madla Paquin & Dupérré, 2009: 28, figs 50-51, 134-135.

Cicurina venii Gertsch, 1992: 111, figs 95-96; syn. n.

Cicurina venii Cokendolpher, 2004: 52, figs 63-64; syn. n.

Cicurina venii Paquin & Dupérré, 2009: 52, figs 116-117; syn. n.

Diagnosis.

The adult morphology of a potentially undescribed sister species ( C. cf. madla ) remains unknown. ME clade members from the neighboring Stone Oak KFR ( C. platypus , C. puentecilla ) have different spermathecal morphologies (large, even-sized, rounded; Cokendolpher 2004, Paquin and Dupérré 2009). Cicurina madla is easily distinguished from neighboring or sympatric R clade members using genetic data, spermathecal morphology, male palpal morphology, or TI index.

Description.

Female spermathecal morphology as described in Paquin and Dupérré (2009). Male palpus with relatively narrow, elongate cymbium, oblong tegulum, origin of embolus at ~ 6 o’clock (Figs 6 , 12 ).

Distribution.

ME clade member known from approximately 25 caves or karst features in the Government Canyon, Helotes, UTSA and northern Culebra Anticline KFRs (Figure 5 ), plus two populations in Stone Oak KFR and Uvalde County (Figure 6 , also figure 4 of Paquin and Hedin 2004).

Discussion.

The high TI index, elongate spermathecae holotype C. venii type specimen is either 1) actually from GCBC, but was mislabeled or placed into an incorrect vial, or 2) is actually from Bracken Bat Cave, and represents a further southern (but currently unknown) extension of the C. madla Culebra Anticline subclade (Figure 11 ). If the latter, sympatry with C. vespera is likely in this central region of the Culebra Anticline.

Figures

Figure 1. Phylogenetic tree from RAxML analysis of 399 UCE loci, 70 % (399 _ 70) occupancy matrix. Bootstrap values below 75 not shown. Geography and morphology highlighted. Abbreviations: ME (= mostly elongate) spermathecae clade, R (= rounded) spermathecae clade. Further details regarding specimen codes and locations found in Suppl. material 5. Inset: sampled Texas counties.

Figure 2. Phylogenetic tree from BEAST analysis of 399 _ 70 UCE matrix. Posterior probability values above 0.95 shown. Inset: Boxplots of TI values (from Suppl. material 6) for ME and R clade members from Bexar County (for ME clade, two small juveniles and Stahl Cave specimens not included).

Figure 3. Phylogenetic tree from RAxML analysis of COI mitochondrial data. Previously published sequences with corresponding GenBank numbers (AY ), some with cave location codes as in Paquin and Hedin (2004). Bootstrap values above 70 shown only for major lineages. Bootstrap values within C. madla shown on Figure 6, those for C. loftini shown on Figure 10.

Figure 4. Morphology of ME clade with corresponding 399 _ 70 UCE RAxML phylogeny (not all bootstrap values shown). Holotype female spermathecal images from Paquin and Duperre (2009), used with permission. Images not to scale.

Figure 5. Distribution of C. madla and C. cf. madla in Bexar and adjacent counties, with corresponding 399 _ 70 RAxML UCE phylogeny (not all bootstrap values shown). Different geographic populations designated by different colors. Cave locations approximate.

Figure 6. Morphology of adult C. madla with corresponding COI RAxML phylogeny. Previously published sequences with corresponding GenBank numbers (AY ), some with cave location codes as in Paquin and Hedin (2004). Imaged specimens highlighted on phylogeny; images of specimens TK _ 188519 and TK _ 188501 from Joel Ledford. Scale bar: 0.5 mm.

Figure 10. Distribution of C. loftini in Culebra Anticline KFR, with corresponding COI RAxML phylogeny. Different mtDNA microclades designated by different colors. Cave locations approximate.

Figure 11. Anomalous geographic distribution of C. venii and C. vespera type specimens. All views ventral.

Figure 12. Male palpal morphology of C. madla and C. vespera (left palp, ventral view). Specimens from Stevens Ranch Trash Hole Cave (CIC _ 1745) and Robbers Cave (TX _ 241) from PP personal collection. Image of specimen TK _ 188519 from Joel Ledford. Abbreviations: CB = cymbium, T = tegulum, E = embolus. Scale bar: 0.5 mm.