Dactylospora chiangraiensis Ekanayaka, E.B.G. Jones, Q. Zhao & K.D. Hyde, 2019

Ekanayaka, Anusha H., G, E. B., Jones, areth, Hyde, Kevin D. & Zhao, Qi, 2019, A stable phylogeny for Dactylosporaceae, Cryptogamie, Mycologie 20 (3), pp. 23-44 : 28-30

Dactylospora chiangraiensis Ekanayaka, E.B.G. Jones, Q. Zhao & K.D. Hyde , sp. nov.

( Fig. 4 )

Black apothecia with maroon to brown margins, ectal excipulum of textura angularis, paraphyses with slightly swollen apices, unitunicate, cylindrical, short pedicellate, inoperculate asci with an amyloid gelatinous cap and smooth one-septate ascospores.

INDEX FUNGORUM NUMBER. — IF555304.

FACESOFFUNGI NUMBER. — FoF 04853.

HOLOTYPE. — MFLU 16-0570 .

TYPE LOCALITY. — Thailand

ETYMOLOGY. — With reference to the province where the holotype was collected.

MATERIAL EXAMINED. — Thailand. Tham Pla Temple M.14 (Bann Tham Pla Temple), Pongpha , Mae Sai District , Chiang Rai Province, 25th November 2014, A. H. Ekanayaka ( HD 003) (holo-, MFLU [ MFLU 16-0570 ]; iso-, HKAS). Sequence data: ITS-MH718440, LSU-MH718433

DESCRIPTION

Saprobic on unidentified wood, stems and twigs.

Sexual morph

Apothecia. 450-500 × 1000-1500 Μm (ẍ = 469.7 × 1242.5 Μm, n =10) superficial, sessile, arising singly or in small groups.

Hamathecium. Cupulate, outer surface black.

Margins. Raised, maroon to brown, rough surface.

Disc. Flat to slightly concave, black, rough granulated surface.

Ectal excipulum. 55-65 Μm wide at margins, comprising cells of textura angularis, outer cell layer is dark brown to light brown, inner cells are hyaline to brownish.

Medullary excipulum. 15-25 Μm wide at margins, composed of brown intertwined hyphae, embedded in a gelatinous matrix.

Pseudo epithecium. Composed of hyaline to brown amorphous matter surrounding the paraphysis tips.

Paraphyses. 1.8-2.3 Μm (ẍ = 2.1 Μm, n =10) wide at the middle, 3.5-5 Μm (ẍ = 4.3 Μm, n =10) wide at the tips, numerous, filamentous, septate, branched, slightly swollen and branched at the apices extending beyond the asci and apices are glued together to form the epithecium.

Asci. 65-75 × 12-15 Μm (ẍ = 69.7 × 13.6 Μm, n =30), 8-spored, unitunicate, cylindrical, short pedicellate, inoperculate with an amyloid gelatinous cap.

Ascospore. 12-15 × 4-4.5 Μm (ẍ = 14.2 × 4.3 Μm, n =40), ellipsoid to fusoid, hyaline at immature stage and brown at maturity, one-septate, smooth walled, guttulate.

Asexual morph

Undetermined.

REMARKS

Dactylospora chiangraiensis sp. nov. is characterized by black apothecia with maroon to brown margins, paraphyses with slightly swollen apices and smooth oneseptate ascospores. Our species is phylogenetically close to D. stygi a (Berk. & M.A. Curtis) Hafellner. However, D. chiangraiensis sp. nov. differs from D. stygia by having maroon to brown raised margins with rough granulated surface in disc, margins and outer surface of apothecia ( Berkeley 1875; Baral & Marson 2005).

Dactylospora parellaria (Nyl.) Arnold, 1877 , D. borealis Holien & Ihlen, 2004 , D. rhyparizae Arnold, 1874 , D. parasitica (Flörke) Arnold, 1887 , D. attendenda (Nyl.) Arnold, 1895 , D. deminuta (Th. Fr.) Triebel, 1989 , D. urceolata (Th. Fr.) Arnold, 1874 , D. frigida Hafellner, 1985 , D. suburceolata Coppins & Fryday, 2012 , D. imperfecta (Ellis) Hafellner, 1979 and D. pertusariicola (Willey ex Tuck.) Hafellner, 1979 differ from D. chiangraiensis sp. nov. by having ascospores with more than 1 septa ( Ihlen et al. 2004). Dactylospora rimulicola (Müll. Arg.) Hafellner, 1979 , D. ahtii Zhurb. & Pino-Bodas, 2017 , D. saxatilis (Schaer.) Hafellner, 1979 , D. homoclinella (Nyl.) Hafellner, 1979 , D. australis Triebel & Hertel, 1989 and D. amygdalariae Triebel, 1989 differ from D. chiangraiensis sp. nov. by having stipitate apothecia ( Ihlen et al. 2004). Dactylospora purpurascens Triebel, 1989 and D. athallina (Müll. Arg.) Hafellner, 1979 differ by having an epihymenium with K+ purple reaction ( Ihlen et al. 2004). Dactylospora lobariella (Nyl.) Hafellner, 1979 and D. protothallina (Anzi) Hafellner, 1979 differ by having a lichenized lifestyle ( Ihlen et al. 2004). Dactylospora inopina Döbbeler & W.R. Buck, 2017 differs by having polyporous asci ( Döbbeler & Buck 2017). Dactylospora aeruginosa Holien & Ihlen, 2004 differs by having an epihymenium with patches of violet-blue pigment ( Ihlen et al. 2004). Dactylospora microspore Etayo, 1991 differs from D. chiangraiensis sp. nov. by having smaller ascospores (4-7 × 2-3 Μm) ( Ihlen et al. 2004; Joshi et al. 2010). Dactylospora vrijmoediae K.L. Pang, Sheng Y. Guo, Alias, Hafellner & E.B.G. Jones, 2014 and D. canariensis Kohlm. & Volkm. -Kohlm., 1998 differs from D. chiangraiensis sp. nov. by having ascospore appendages ( Jones et al. 1999; Pang et al. 2014). Dactylospora mangrovei E.B.G. Jones, Alias, Abdel-Wahab & S.Y. Hsieh, 1999 and D. haliotrepha (Kohlm. & E. Kohlm.) Hafellner, 1979 differ from D. chiangraiensis sp. nov. by having ascospore wall ornamentation ( Au et al. 1996; Jones et al. 1999).

Figures

FIG. 4. — Morphology of Dactylospora chiangraiensis Ekanayaka, E.B.G. Jones, Q. Zhao & K.D. Hyde, sp. nov. (Holotype MFLU 16-0570): A, B, ascomata in wood; C, ascoma in wood; D, cross section of a ascoma; E, peridium of ascoma; F-I, asci in water; J, paraphyses in water; K, ascus apex with gelatinous amyloid cap (in Melzer’s reagent); L-O, ascospores in water; P, germinated ascospore. Scale bars: A, 1000 µm; B, 500 µm; C, D, 200 µm; E, 100 µm; F-I, 25 µm; J, P, 50 µm; K-O, 10 µm.