Diaporthe pseudofoliicola Q. Yang, 2024

Liu, Hong Y., Luo, Dun, Huang, Han L. & Yang, Qin, 2024, Two new species of Diaporthe (Diaporthaceae, Diaporthales) associated with Camellia oleifera leaf spot disease in Hainan Province, China, MycoKeys 102, pp. 225-243 : 225

Diaporthe pseudofoliicola Q. Yang sp. nov.

Fig. 3

Diagnosis.

Distinguished from D. longicolla in having smaller alpha conidia; from D. unshiuensis in having narrower conidiophores.

Etymology.

The epithet " Diaporthe pseudofoliicola " refers to its habitat similar to Diaporthe foliicola .

Description.

Asexual morph: Conidiomata on PDA pycnidial, 190-330 μm in diam., superficial, scattered on PDA, dark brown to black, globose, solitary or clustered in groups of 1-3 pycnidia. Pale yellow conidial drops exuding from ostioles. Conidiophores reduced to conidiogenous cells. Conidiogenous cells (10.5-)12.5-18(-22) × 1.3-1.5 μm (n = 30), phialidic, aseptate, cylindrical, straight, densely aggregated, terminal, slightly tapered towards the apex. Alpha conidia 5-6.5(-7) × 2.3-3.0 μm (n = 30), aseptate, hyaline, ellipsoidal to fusiform, biguttulate, both ends obtuse. Beta conidia (27.5-)30-33(-35.5) × 1.2-1.4 µm (n = 30), hyaline, aseptate, filiform, sinuous at one end, eguttulate.

Culture characters.

Culture incubated on PDA at 25 °C, originally flat with white fluffy aerial mycelium, becoming pale brown due to pigment formation, with yellowish-cream conidial drops exuding from the ostioles after 20 days.

Specimens examined.

China. Hainan Province: Chengmai County, on leaves of Camellia oleifera , 110°15'16"E, 19°23'20"N, 25 July 2022, Q. Yang (holotype CSUFT050; ex-type living culture: HNCM045; other living cultures: HNCM046, HNCM047 and HNCM048) .

Notes.

Four isolates representing D. pseudofoliicola cluster in a well-supported clade (ML/BI = 100/1) and appear most closely related to D. longicolla on Glycine max and D. unshiuensis on Citrus unshiu . Diaporthe pseudofoliicola can be distinguished from D. longicolla , based on ITS, tef1 and tub2 loci (9/462 in ITS, 16/318 in tef1 and 4/444 in tub2); from D. unshiuensis , based on his3 and tef1 loci (51/457 in his3 and 17/318 in tef1). Morphologically, D. pseudofoliicola differs from D. longicolla in having smaller alpha conidia (5-6.5 × 2.3-3.0 μm vs. 6.9-7.2 × 1.6-2.8 μm) ( Santos et al. 2011); from D. unshiuensis in having narrower conidiophores (1.3-1.5 μm vs. 1.4-2.6 μm) ( Huang et al. 2015).

Figures

Figure 3. Diaporthe pseudofoliicola (HNCM 045) A culture on PDA B, C conidiomata D conidiogenous cells E alpha and beta conidia. Scale bars: 200 μm (B, C), 10 μm (D), 20 μm (E).