Sphingomonas crusticola KG

Soung, Seung Hyeok, Song, Jaeho & Han, Seung Yeol Shin and Song-Ih, 2023, A report of 20 unrecorded bacterial species in Korea, isolated from soils of coastal areas in 2022, Journal of Species Research 12 (4), pp. 267-276 : 273

Description of Sphingomonas crusticola KG 1R52

Cells are Gram-stain-negative, aerobic, flagellated, and rod shaped. Colonies are circular, convex, entire, and yellow colored after incubation for 3 days on MA at 25℃. Positive for β-galactosidase and acetoin production; but negative for arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, citrate utilization, H 2 S production, urease, tryptophane deaminase, indole production, and gelatinase in API 20E. D-Glucose, D-sucrose, D-melibiose, amygdalin, and L-arabinose are utilized for fermentation/oxidation; but not D-mannitol, inositol, D-sorbitol, and L-rhamnose. In the API 50 CH test, positive for L-arabinose, D-xylose, methyl-α D-glucopyranoside, D-galactose, D-glucose, D-fructose, D-mannose, N -acetylglucosamine, amygdalin, arbutin, esculine, salicin, D-cellobiose, D-lactose, D-melibiose, D-saccharose, D-trehalose, D-melezitose, glycogen, xylitol, gentiobiose, D-lyxose, D-fucose, and potassium 5-ketogluconate. The API ZYM test is positive for alkaline phosphatase, esterase, lipase esterase, leucine arylamidase, valine arylamidase, α-chymotrypsin, acid phosphatase, naphtol-as-bi-phosphohydrolase, α-galactosidase, β-glucuronidase, β-glucosidase, α-glucosidase, β-glucosidase, and N - acetyl-β-glucosaminidase. Strain KG1 R52 (= HNIBRBA4483) was isolated from rhizosphere soils of a halophyte ( Calystegia soldanella ) collected from Anmyeon-do (36°33′ 38.25″N, 126°19′23.05″E), Taean , Chungcheongnam-do, Korea. The GenBank accession number of the 16 S rRNA gene sequence of strain KG1 R52 is OP602333 .