Eukerria barrigosii James, Bartz & Brown, 2023

James, Samuel W., Bartz, Marie L. C. & Brown, George G., 2023, New Ocnerodrilidae genera, species and records from Brazil (Annelida: Crassiclitellata), Zootaxa 5255 (1), pp. 235-269 : 240-241

Eukerria barrigosii James, Bartz & Brown , sp. nov. ( Fig. 3 a-c)

Holotype. BRMS0115 , one adult, Miranda , Mato Grosso do Sul state, Brazil, S20.2614°, W56.4168°, 114 m asl, in flooded rice field; 27 September 2006, J.A. Barrigosi, coll.

Paratypes. BRMS0013 , one adult, two pre-clitellates, same data as holotype . BRMS0007 , one adult, same data as holotype .

Etymology. The species is named in honor of its collector, José Antonio Barrigosi, who has sent us several lots of specimens from the region.

Description. Dimensions 53-73 mm by 1.2-1.4 mm at segment x, 1.0- 1.2 mm at xxx, 1.2-1.5 mm at clitellum; body cylindrical throughout, segments 84. Setae closely paired throughout, CD sub-lateral; setal formula AA:AB: BC:CD = 4:1:4.5:1 at xxx, DD> ½ circumference. Setae AB present unmodified in xvii-xix. Prostomium epilobous open; segments without secondary annulations. Unpigmented, no dorsal pores, spermathecal pores in 7/8, 8/ 9 in BC just below C. Ovipores in xiv just behind 13/ 14 in B line. Male pores in seminal grooves in xviii; prostatic pores at ends of grooves lateral to B in xvii-xix; seminal grooves, prostatic pores on longitudinal elevated pads aligned about the AB distance lateral to B in xvii-xix. Clitellum xiii- xxii, saddle-shaped, genital markings mid-ventral in BB from equator of ix to equator of x, mid-ventral in AA from equator of xv to 16/17, mid-ventral in AA from equator of xx to equator of xxii, or only to 21/22 ( Fig. 3a ).

Anterior septa 5/6-8/9 thickly muscular, others membranous. Alimentary canal with distinct gizzard in vii; esophagous valvular in xi, intestinal origin ½ xii; no typhlosole, slight intestinal constriction at 21/22. Calciferous glands paired in ix, elongate with ascending duct, attached latero-dorsally to esophagus. Glands with thick wall composed of longitudinal tubules, central lumen present ( Fig. 3b ); each gland with blood vessel from anterior end to extra-esophageal vessel, blood vessel from posterior face of gland to supra-esophageal vessel. Holonephric, tubular nephridia with dense white mantle filling segmental cavities from A to mid-dorsal, first nephridia in xii.

Vascular system with ventral trunk, single dorsal trunk, lateral vessels in vi-ix, large latero-esophageal hearts in x, xi.

Fan-shaped ovaries, free with funnels in xiii; paired spermathecae in viii, ix, each an irregular sac ampulla on long slender duct with thicker section at ampulla junction, sperm chambers within this thicker part of the duct, no diverticulum; spermathecae of viii smaller ( Fig. 3c ).

Male sexual system proandric, testes and funnels in x free, vas deferens superficial on body wall from 10/11 to xviii, iridescent and enlarging in xvii-xviii, terminating in small round chamber on body wall of xviii, seminal vesicles in ix with several long stalked branches, placed laterally on body wall, large and post-septal in xi; tubular prostates folded under intestine within xvii-xxiii, xxxv, xxxvii; prostatic ducts ectally enlarged, muscular, no penial setae.

Remarks: The new species is geographically and morphologically close to E. subandina ( Rosa, 1895) from the same region of Brazil but on the opposite side of the Pantanal (Urucum; Jamieson 1970). Eukerria barrigosii sp. nov. is diagnosed by the saddle-shaped longer clitellum ending on xxii, whereas most others terminate in xix or xx, lack of copulatory bursae at the prostatic pores, spermathecal pores below C in BC, lack of genital markings, spermathecae not in spiral form and with ducts shorter than ampulla, the first muscular septum 5/6, and the first nephridia in xii. Unfortunately, no DNA barcode data is available for this species.

Figures

FIGURE 3. Eukerria barrigosii sp. nov. A. Ventral view with genital marking (gm) and male field (mf), scale bar 1 mm; B. Cross section of calciferous gland; C. Spermatheca of segment ix, scale bar 0.4 mm.