Periglischrus caligus Kolenati, 1857

Gomes-AlmeidaK, Brenda Karolina, DiórioK, Gabriel Félix, CostaK, Samuel Geremias dos Santos & PepatoK, Almir Rogério, 2024, DNA barcoding, visual-guide resource, new localities and host associations of genus Periglischrus Oudemans, 1902 (Acari: Mesostigmata, Spinturnicidae) from Minas Gerais, Brazil, Acarologia 1902 (2), pp. 425-462 : 436-441

Periglischrus caligus Kolenati ( Figures 6–8 )

Periglischrus caligus Kolenati, 1857: 60 (original designation).

Periglischrus setosus Machado-Allison 1964: 199–200 .

Specimens examined — 2♀ (UFMG AC 220058, 221031), 2♂ (UFMG AC 220066,

221035) and 3 protonymphs (UFMG AC 220057, 220059–60) on bat Glossophaga soricina (4

ex.): Lagoa Santa, Pacas cave, PE Sumidouro, - 19.5606° S, - 43.9667° E, 12 Aug. 2021, collected by B. Almeida et al. (COX1 sequence [voucher code]: OP964376 [UFMG AC 220066], OP964377 [UFMG AC 221035], OP964379[UFMG AC 221031], OP964395[UFMG AC

220060], OP964396[UFMG AC 220059], OP964397[UFMG AC 220058], OP964398[UFMG with –

proteronotal setae (Pn1–Pn5) and poststigmal seta Pst () indicated; D – Dorsal opisthosoma with hysteronotal setae (Op3–Op6) indicated in red arrow; E and F – Sternal plate with sternal setae (St1–St4); G – Proximal anterodorsal ad () seta on femur–tibia I; H – Distal posteroventral (pv)

seta on femur–tibia I (enlarged and serrate) and tarsus I (small and spinelike), indicated in red arrow; I – Proximal ad anteroand) (posterodorsal (pd) setae on tibia II; J – Proximal ad and pd setae on femur II; K – Distal pv setae on femur–tibia II (enlarged and serrate) and tarsus II (small and spinelike), indicated in red arrow; L – Proximal ad and pd setae on femur IV; M and N – Posterolateral pl () setae on femur–tibia IV with details, indicated in red arrow. Scale bars: A =200 µm, B, F–N = 50 µm, C–E = 100 µm.

AC 220057]); 4♂ (UFMG AC 220107–09, 220808) and 2 protonymphs (UFMG AC 220124,

221032) on Glossophaga soricina (3 ex.): Montes Claros, Lapa Grande cave, PE da Lapa Grande, - 16.7067° S, - 43.9549° E, 13-14 Dec. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964378[UFMG AC 221032], OP964381[UFMG AC

220808], OP964390[UFMG AC 220124], OP964391[UFMG AC 220109], OP964392[UFMG

AC 220108], OP964393[UFMG AC 220107]); 2♀ (UFMG AC 220214, 220218), 1♂ (UFMG

AC 221016) and 1 protonymph (UFMG AC 220216) on Glossophaga soricina (2 ex.): Brazil, Minas Gerais, Rio Pardo de Minas, Chácara cave (unregistered), PE Serra Nova e Talhado, - 15.6758° S, - 42.7295° E, 20 Dec. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964380[UFMG AC 221016], OP964382[UFMG AC 220216], OP964383[UFMG AC 220214]); 2♀ (UFMG AC 220147, 220149) and 1♂ (UFMG AC 220148)

on Glossophaga soricina (1 ex.): Brazil, Minas Gerais, Rio Pardo de Minas, Mosquito cave (unregistered), PE Serra Nova e Talhado, - 15.6545° S, - 42.7335° E, 16 Dec. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964388 [UFMG AC 220148]

and OP964389[UFMG AC 220147]); 2♀ (UFMG AC 220186, 220181), 1♂ (UFMG AC

220185) and 2 protonymphs (UFMG AC 220183-84) on Glossophaga soricina (2 ex.): Brazil, Minas Gerais, Rio Pardo de Minas, Pequenos Labirintos cave (unregistered), PE Serra Nova e Talhado, - 15.6293° S, - 42.7043° E, 18 Dec. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964384–87); 1♂ (UFMG AC 220104) on Glossophaga soricina (1 ex.): Brazil, Minas Gerais, Santana do Riacho, Bocaina V cave, APA Morro da Pedreira, - 19.3346° S, - 43.6032° E, 17 Sep. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964394 [UFMG AC 220104]).

Barcode sequences — OP964376–78, OP964381, OP964383–92, OP964394, OP964397

( Table 1).

Distribution — Bolivia, Brazil, Mexico, Panama, Peru, Suriname, Venezuela ( Beron

2020).

Hosts and records from Brazil — Artibeus lituratus (Olfers, 1818) : Rio de Janeiro ( Lourenço et al. 2020); Artibeus planirostris : Mato Grosso do Sul ( Silva and Graciolli 2013 ; Silva et al. 2017); Glossophaga soricina (Pallas, 1766) : Ceará, Mato Grosso ( Almeida et al. 2016b), Rio de Janeiro, São Paulo ( Confalonieri 1976), Distrito Federal ( Gettinger and Gribel 1989), Brazil ( Rudnick 1960), Mato Grosso do Sul ( Silva and Graciolli 2013 ; Silva et al. 2017), Rio Grande do Sul ( Silva et al. 2009) and Minas Gerais (new report in present study); Platyrrhinus lineatus : Mato Grosso do Sul ( Silva and Graciolli 2013); unknown bat: Mato Grosso ( Confalonieri 1976).

Differential diagnosis — Female: four pairs of small to minute setae on dorsal opistonothal area ( Figure 6D ); sternal plates subpentagonal, narrow anterior border with anterior projection narrowly rounded ( Figure 6E–F ); posterolateral pl () setae on femur-tibia IV greatly inflated,

with slender recurved end ( Figure 6M–N ). Male: sternogenital seta St1 small, extending posteriorly about two-thirds the distance to first pair of pores; intercoxal IV area bears eight pairs of setae with first pair as microsetae posterior to sternogenital plate ( Figure 7C ); coxa

II with seta pv much shorter than the width of coxa II ( Figure 7I ) ( Herrin and Tipton 1975 ; Morales-Malacara 2001; Morales-Malacara and López-Ortega 2001). Protonymph: similar to males, except by ontogenetic differences ( Deunff et al. 2011) ( Figures 8A–N ).

Remarks — P. caligus is reported for the first time to Minas Gerais fauna. This is a stenoxenous species on glossophagine bats of the genus Glossophaga . Morphological characters of examined specimens agree with original description and re-descriptions ( Machado-Allison 1964 ; Herrin and Tipton 1975 ; Furman 1966). Periglischrus caligus protonymph may be misidentified as P. herrerai that differs by having first pair of setae on intercoxal area IV small (minute in P. caligus , Figures 8C and 10C ); tarsus III with proximal posterodorsal pd ()

seta medium to small (vs. large) and proximal anterodorsal ad () seta similar in size with pd

(vs. small, Figure 8K and 10K ); Proximal anterodorsal ad () on femur IV medium (vs. small, Figure 8L and 10L ) and proximal antero and posterodorsal setae on genu IV medium (vs. small, Figure 8M and 10M ).

In our bGMYC species delimitation analyses ( Fig. 2 ) Periglischrus caligus is represented by 20 haplotypes (11 to 30) out of 23 sequences and all haplotypes were recovered as a single putative species with pp.> 95%.

Figures

Figure 2 Mitochondrial COI haplotype ultrametric tree inferred in BEAST using strict clock and species delimitation probabilities obtained in bGMYC.

Figure 6 Periglischrus caligus, female. A – General view; B – Mediodistal lobe of palpal tibia indicated in red arrow; C – dorsal plate

Figure 7 Periglischrus caligus, male. A – General view; B – Dorsal view with proteronotal setae (Pn1–Pn5) and poststigmal seta (Pst) indicated; C – Ventral view with sternogenital setaeSt(1–St4) and genital seta (Sg) indicated; D – Ventral setae on legs I and II with details; E Details ventral setae on leg I; F – Details ventral setae on leg II; G – Coxa I with pv anddvsetae, indicated in red arrow; H – Femur–tibia I with proximal adsetae, indicated; I – Coxa II with posterolateral setapl () indicated; J – Femur II, proximaladandpdsetae, indicated; K – Proximal adandpdon tarsus III, indicated; L – Femur IV, proximaladandpdsetae, indicated; M – Genu IV, proximaladandpdsetae, indicated; N – Proximal adandpdon tarsus IV, indicated. Scale bars: A =200 µm, B, C, E–N = 50 µm, D = 100 µm.

Figure 8 Periglischrus caligus, protonymph. A – General view; B – Dorsal view; C – Ventral view; D – Ventral setae on legs I and II with details; E – Details ventral setae on leg I; F – Details ventral setae on leg II; G – Coxa I withpv anddvsetae, indicated; H – Femur–tibia I with proximal adsetae, indicated; I – Coxa II with posterolateral setapl () indicated; J – Femur II, proximaladandpdsetae, indicated; K – Proximal adandpdon tarsus III, indicated; L – Femur IV, proximaladandpdsetae, indicated; M – Genu IV, proximaladandpdsetae, indicated; N – Proximal adandpdon tarsus IV, indicated. Scale bars: A =200 µm, B–N = 50 µm.

Figure 10 Periglischrus herrerai, protonymph. A – General view; B – Dorsal view; C – Ventral view; D – Ventral setae on legs I and II with details; E – Details ventral setae on leg I; F – Details ventral setae on leg II; G – Coxa I withpv anddvsetae, indicated; H – Femur–tibia I with proximal adsetae, indicated; I – Coxa II with posterolateral setapl () indicated; J – Femur II, proximaladandpdsetae, indicated; K – Proximal adandpdon tarsus III, indicated; L – Femur IV, proximaladandpdsetae, indicated; M – Genu IV, proximaladandpdsetae, indicated; N – Proximal adandpdon tarsus IV, indicated. Scale bars: A–N = 50 µm.