Martiodrilus (Maipure) quimbayaensis, Feijoo & Zuluaga & Molina, 2018

Feijoo, Alexander, Zuluaga, Luis Fernando & Molina, Ligia Janneth, 2018, New species and records of earthworms (Annelida, Oligochaeta) in plantain cropping systems in Colombia's coffee-growing region, Zootaxa 4496 (1), pp. 448-458 : 452-453

Martiodrilus (Maipure) quimbayaensis , sp. nov.

( Figure 3A–C )

Material examined. Holotype: Clitellate specimen (CNLT-UTP-0320). Locality : Colombia, Department of Quindío, La Sofe farm (4° 33'30"N, 75°41'10"W). Type of vegetation: sole cropped plantain, intercropped coffee/ plantain crops. Altitude : 1479 m.a.s.l. Sampling depth: 0–10 cm litter. Collection dates: 26 April 2016 and 1 June 2017. Collectors: A. Feijoo and L.J. Molina . Paratypes: Three adults (CNLT-UTP-0321). Locality: Colombia, Department of Quindío, La Sofe farm (4° 33'30"N, 75°41'10"W). Type of vegetation: sole croppd plantain, intercropped coffee/plantain crops. Altitude : 1479 m.a.s.l. Sampling depth: 0–10 cm litter. Collection dates: 26 January 2015, 26 April 2016 and 31 June 2017. Collectors: A. Feijoo (2015) and A. Feijoo and L.J. Molina, other dates. Other materials: three juveniles and 7 adults (CNLT-UTP-0322), same collection data as holotype .

Etymology. The name of the species is given because of an ancient indian group dominant in the Colombian coffee-growing region.

Description. Epigeic species, with a light-blue to Bermuda grey-colored dorsum, milky white ventrum, and cylindrical body. Holotype: length (preserved) 74.2 mm, diameter 2.3 mm in preclitellar region (segment 8), 3.3 mm at clitellum, 2.9 mm in mid-posterior region. Number of segments: 103. Paratype (three adults): Adult 1: 56.6 mm long, diameter 3 mm in preclitellar region, 2.8 mm at clitellum, 2.6 mm in mid-posterior region, number of segments: 70. Adult 2: 88.7 mm long, diameter 2.8 mm in preclitellar region, 3.2 mm at clitellum, 2.8 mm in midposterior region; number of segments: 119. Adult 3: 94.8 mm long, diameter 2.8 mm in preclitellar region, 3.94 mm at clitellum, 2.3 mm in mid-posterior region; number of segments: 115. The prostomium short and lobeshaped. Eight rows of closely paired setae are visible (a, b, c, d) as of segment 3. Common setae slightly sigmoid with a slightly pronounced central nodule, tips at both ends almost rounded. Distance between setae in mid-body region (segments 40–50); aa:ab:bc:cd:dd = 4.4:1:4.9:0.6:20.9. Genital setae in segments 5–9, 15–23, with transverse excavations in proximal 2/3, in distal 1/3 semilunar marks; distally with a spear-shaped tip ( Fig. 3A ). Clitellum whitish (preserved), saddle-shaped, in segments 14–1/2 23 (=9.5 segments); tubercula pubertatis between segments ½ 19–½ 26 ( Fig 3B ). Male pores in segment 20/21, female pores in segment 13/14. Nephropores aligned with setae c. Four pairs of spermathecal pores observed in intersegments 5/6, 6/7, 7/8, 8/9.

Internal anatomy. Anterior septa very fragile: only tiny membranous threads representing the septa are observed between segments 5 and 12. Gizzard rounded, in segment 6. Seven pairs of small extramural, tubularshaped calciferous glands, opening into dorso-ventral region of esophagus in segments 7–13; all glands of similar size, with a small appendage in the distal region highly irrigated with blood; gland structure of composite-tubular type, consisting of short parietal tubules that flow into each other. In the center, the tubules merge together in the lumen from where they branch out toward the tip of the calciferous gland. The lumen narrows in the submedial region and tubules close irregularly. Transition esophagus-intestine at 17/18, typholosole beginning in segments 26–27. No caeca. Three pairs of thin lateral hearts in segments 7–9, separating the calciferous glands, and two pairs of voluminous intestinal hearts in segments 10 and 11. One pair of holonephridia per segment, nephridiopores located just above the line of setae c. Two pairs of large hypo-esophageal testis sacs in segments 10 and 11, two pairs of seminal vesicles extending forward in segments 11 and 12. One pair of ovaries in segment 13. Four pairs of spermathecae that increase in size, 9>8>7>6, near setae c. Each spermatheca with conspicuous duct and ampulla visible from the outside; duct short, ampulla an elongate mass; no seminal chambers or diverticles ( Fig. 3C ).

Remarks. Martiodrilus (Maipure) quimbayaensis sp. nov. is a species closely related to M. (Mp.) ecuadoriensis (Benham, 1892) , M. (Mp.) grandis Zicsi, 2001 , M. (Mp.) huitoto Feijoo & Celis, 2011 , M. (Mp.) palmirus Zicsi & Feijoo, 1994 and M. (Mp.) savanicola (Michaelsen 1900) , because of the presence of seven pairs of calciferous glands (in segments 7–13 or 8–14, depending on the species) and four pairs of spermathecae. However, these species differ from the new species in the following characteristics: M. (Mp.) ecuadoriensis : clitellum in segments 15–½ 25 (vs. 14 –½ 23), tubercula pubertatis in segments 21–27 or ½ 20–½ 27 (vs. ½ 19–½ 26); spermathecae bag-shaped and without distinction between duct and ampulla; shape and size of normal setae, and ornamentation of genital setae. M. (Mp.) grandis : clitellum in segments 14–½ 27, 27, (vs. 14–½ 23), tubercula pubertatis in segments ½ 20–½ 27 (vs. ½ 19–½ 26), shape of spermathecae. M. (Mp.) huitoto : clitellum in segments 14, 15–28 (vs. 14–½ 23), tubercula pubertatis in segments ½ 19–½ 26 (vs. ½ 19–½ 26), as well as shape of spermathecae. M. (Mp.) palmirus : clitellum in segments 15–½ 25 (vs. 14–½ 23), tubercula pubertatis in segments 20–¼ 25 (vs. ½ 19–½ 26), calciferous glands in segments 8–14 (vs. 7–13). M. (Mp.) savanicola : clitellum in segments 15–24 (vs. 14–½ 23), tubercula pubertatis in segments 20–¼ 27 (vs. ½ 19–½ 26), nephropores aligned with setae d (vs. aligned with setae c), calciferous gland in segments 8–14 (vs. 7–13).

Figures

FIGURE 3. Martiodrilus (Maipure) quimbayaensis sp. nov., holotype. A. Genital seta a, segment 22. B. External, ventral view of tubercula pubertatis, segments ½ 19–½ 26; C. Internal anatomy of segments 5–12, dorsal view. S= spermathecae of 6, 7, 8, 9; G= gizzard in segment 6.