Martiodrilus (Botaria) tchoukouchipan Decaëns & Bartz, 2024

Decaëns, Thibaud, Bartz, Marie Luise Carolina, Bartz, arolina, Feijoo-Martínez, Alexander, Goulpeau, Arnaud, Lapied, Emmanuel, Marchán, Daniel F., Maggia, Marie-Eugénie, Papuga, Guillaume & James, Samuel W., 2024, Earthworms (Oligochaeta, Clitellata) of the Mitaraka range (French Guiana): commented checklist with description of one genus and eighteen species new to science, Zoosystema 46 (9), pp. 196-244 : 211-213

Martiodrilus (Botaria) tchoukouchipan Decaëns & Bartz , n. sp.

( Figs 2E ; 10 )

urn:lsid:zoobank.org:act:D2235D38-95FC-44C7-8FDE-CD45AC4F308F

TYPE MATERIAL. — Holotype. French Guiana • Adult specimen; Tumuc-Humac, Mitaraka Massif , plateau forest on DIADEMA project C trail, in the soil; 2°13’58”N, 54°26’38”W; 449 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0289 ; MNHN.

Paratypes. French Guiana • 2 adult specimens; Tumuc-Humac, Mitaraka Massif , plateau forest at base camp; 2°14’2”N, 54°27’1”W; 331 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0285 , EW-MI15-0303 ; CEFE • 1 adult specimen; Tumuc-Humac, Mitaraka Massif , plateau forest on DIADEMA project C trail; 2°13’58”N, 54°26’38”W; 449 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0298 ; CEFE • 1 adult specimen; Tumuc-Humac, Mitaraka Massif , rocky savannah #1 on Sommet en Cloche Inselberg ; 2°13’40”N, 54°28’1”W; 623 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0362 ; CEFE • 3 adult specimens; Tumuc-Humac, Mitaraka Massif , rocky savannah #2 on Sommet en Cloche Inselberg ; 2°13’58”N, 54°27’39”W; 427 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0324 , EW-MI15-0379 , EW-MI15-0157 ; MNHN • 1 adult specimen; Tumuc-Humac, Mitaraka Massif , transition forest # 1 Sommet en Cloche Inselberg ; 2°13’54”N, 54°27’39”W; 401 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; EW-MI15-0336 ; MNHN • 1 adult specimen; Tumuc-Humac, Mitaraka Massif , transition forest on Tchoukou Chipan Inselberg ; 2°14’54”N, 54°29’12”W; 405 m a.s.l.; III.2015; C. Baraloto leg.; BOLD Sample ID: EW-MI15-0108 ; MNHN • 1 adult specimen; Tumuc-Humac, Mitaraka Massif , tropical rainforest on crowned mountain; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0127 ; MNHN.

OTHER MATERIAL EXAMINED. — French Guiana • 3 adult specimens; Tumuc-Humac, Mitaraka Massif , hilltop forest on Sommet en Cloche Inselberg ; 2°13’42”N, 54°28’2”W; 638 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0152 , EW-MI15-0153 , EW-MI15-0154 ; MNHN • 4 juvenile specimens; Tumuc-Humac, Mitaraka Massif , plateau forest on DIADEMA project C trail; 2°13’58”N, 54°26’38”W; 449 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0287 , EW-MI15-0288 , EW-MI15-0290 , EW-MI15-0292 ; MNHN • 13 juvenile specimens; Tumuc-Humac, Mitaraka Massif , plateau forest on DIADEMA project D trail; 2°12’57”N, 54°27’25”W; 381 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0020 , EW-MI15-0021 , EW-MI15-0022 , EW-MI15-0092 , EW-MI15-0093 , EW-MI15-0095 , EW-MI15-0096 , EW-MI15-0097 , EW-MI15-0098 , EW-MI15-0101 , EW-MI15-0102 , EW-MI15-0103 , EW-MI15-0104 ; MNHN • 4 adult specimens, 3 juvenile specimens, 5 cocoons; Tumuc-Humac, Mitaraka Massif , rocky savannah #1 on Sommet en Cloche Inselberg ; 2°13’40”N, 54°28’1”W; 623 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0155 , EW-MI15-0156 , EW-MI15-0158 , EW-MI15-0159 , EW-MI15-0165 , EW-MI15-0166 , EW-MI15-0167 , EW-MI15-0168 , EW-MI15-0169 , EW-MI15-0160 , EW-MI15-0162 , EW-MI15-0163 ; MNHN • 3 adult specimens, 4 juvenile specimens, 2 cocoons; Tumuc-Humac, Mitaraka Massif , rocky savannah #2 on Sommet en Cloche Inselberg ; 2°13’58”N, 54°27’39”W; 427 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0320 , EW-MI15-0322 , EW-MI15-0323 , EW-MI15-0331 , EW-MI15-0332 , EW-MI15-0325 , EW-MI15-0326 , EW-MI15-0327 , EW-MI15-0328 ; MNHN • 4 juvenile specimens; Tumuc-Humac, Mitaraka Massif , slope forest on DIADEMA project D trail; 2°13’40”N, 54°27’14”W; 339 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0265 , EW-MI15-0266 , EW-MI15-0267 , EW-MI15-0268 ; MNHN • 1 adult specimen, 2 juvenile specimens, 1 fragment; Tumuc-Humac, Mitaraka Massif , transition forest # 1 Sommet en Cloche Inselberg ; 2°13’54”N, 54°27’39”W; 401 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0313 , EW-MI15-0354 , EW-MI15-0314 , EW-MI15-0338 ; MNHN • 4 adult specimens; Tumuc-Humac, Mitaraka Massif , transition forest # 2 Sommet en Cloche Inselberg ; 2°13’59”N, 54°27’34”W; 395 m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0110 , EW-MI15-0111 , EW-MI15-0112 , EW-MI15-0113 ; MNHN • 1 adult specimen; Tumuc-Humac, Mitaraka Massif , tropical rainforest on crowned mountain; NA/NA; NA m a.s.l.; III.2015; T. Decaëns, E. Lapied leg.; BOLD Sample ID: EW-MI15-0129 ; MNHN.

ETYMOLOGY. — This species is named in reference to the Tchoukou Chipan inselberg near which the holotype specimen was collected.

ECOLOGY. — Martiodrilus (B.) tchoukouchipan Decaëns & Bartz , n. sp. has been found in a wide range of well-drained habitats, with a marked preference for rocky savannahs of the inselbergs (38.46% of specimens), plateau forests (32.31%) and inselberg transition forests (15.38%) ( Fig. 10D ). Most specimens were collected under terrestrial bromeliads covering rocky savannahs (38.46% of specimens), in the organo-mineral soil (38.46%) and a lower proportion in decaying trunks (16.92%) and in litter accumulating on large granite rocks (6.15%) ( Fig. 10E ). Cocoons are tan yellow, spherical, 4.5-5.5 mm in diameter, with two mucrons at the anterior and posterior ends.

DISTRIBUTION. — Martiodrilus (B.) tchoukouchipan Decaëns & Bartz , n. sp. is only known from the Mitaraka Massif.

DESCRIPTION

External morphology ( Fig. 10A, B )

Body shape cylindrical. Body pigmentation dorsally yellow-brown, ventrally yellow.Body length: 56 mm in the holotype, 55 to 75 mm in the paratypes (average: 76.3 mm; n = 6), after ethanol fixation. Body mass: 0.32 mm after ethanol fixation in the holotype, 0.31 to 0.82 g in the paratypes (average: 0.54 g; n = 6). Diameter: 3 to 5.5 mm in the preclitellar region, 3.9 to 5.5 mm in the clitellum, 3.2 to 4.7 mm in the postclitellar region. Number of segments: 96 in the holotype, 101 to 145 in the paratypes (average: 128.3; n = 6). Prostomium proepilobic. One pair of longitudinal grooves passing through segment I in line of nephridial pores in II. Setae closely paired, ab and cd beginning in III or IV. Setal arrangement aa:ab:bc:cd:dd = 6:1:9:1:31. Clitellum in (dorsal XIV) XV (XVI)-½ XXV, saddle-shaped ( Fig. 10A ). Genital markings variable in V-VIII and in X-XII, and intraclitellar in XV-XVIII (ab position). Tubercula pubertatis linear in 1/4XX-1/3XXV ( Fig. 10B ). Male pores not recognised externally, internally open in 19/20, and ovipores not visible. Spermathecal pores in 5/6, 6/7 and 7/8. Nephridial pores begining in II, in CD line.

Internal anatomy ( Fig. 10C )

Septa: membranous. Gizzard: muscular and well developed in VI, but displaced to X, XI, with an average size (width × length) of 2.60 × 3.38 mm. Calciferous glands: eight pairs in VII-XIV, those in X and XI slightly smaller; all kidney-shaped with a terminal appendix and tubular composite structure. Esophagus-intestine transition in XVII/XVIII; intestine without caeca. Typhlosole abruptly begining in XXVI, structured as a long-folded lamella occupying all the lumen. Hearts: six pairs, the two intestinal pairs in X-XI and well developed. Excretory apparatus holoic, nephridia with simple nephrostome.Testes sacs:midventral or hypoesophagic in X and XI, enclosing seminal vesicles. Seminal vesicles: two pairs in XI-XII: first pair enclosed in septa extending to IX and X; second pair going until XVII, lobulated with division in the segments and attached to the dorsal body wall. Spermathecae: three pairs, VI, VII and VIII, elongated without diverticula.

REMARKS

Martiodrilus (B.) tchoukouchipan Decaëns & Bartz , n. sp. belongs to the subgenus Botaria because of the presence of 3 pairs of spermathecae. It resembles a group of species sharing a clitellum positioned on XV-XXV, including M. (B.) pavliceki Bartz & Decaëns , n. sp., M. (B.) bicolor , M. (B.) euzonus , M. (B.) gara , M. (B.) gravis , M. (B.) minoriformis , M. (B.) poncei and M. (B.) tutus . However, it differs from these species in the position of the tubercula pubertatis in 1/4XX-1/3XXV which is only compatible with the description of M. (B.) gravis and M. (B.) tutus . It is finally distinguished from the latter two species by its significantly smaller size (55-75 mm, whereas M. (B.) gravis and M. (B.) tutus are larger than 90 mm and 100 mm, respectively). Martiodrilus (B.) tchoukouchipan Decaëns & Bartz , n. sp. is clearly separated from the other species of Martiodrilus from the Mitaraka Massif by its COI barcode ( Table 2 ). This species corresponds to OTU#071 in Maggia et al. (2021).

Figures

FIG. 2. — (next page) The Mitaraka Massif: A, general view of the inselberg landscape from “Borne 1”, with “Sommet en Cloche” in the foreground, followed by “Tchoukouchipan” and “Mitaraka”;B, Astrocaryum forest plateau near Base Camp;C, transitional forest (TF1) on the slopes of the “Sommet en Cloche”;D, Rocky savannah (RS3) showing terrestrial bromeliad (Pepinia sp.) and transitional forest (TF2) in the background; E, a specimen of Martiodrilus (Botaria) tchoukouchipan Decaëns & Bartz n. sp. in the root mat of terrestrial bromeliads in a rocky savannah (RS2); F, the holotype of Martiodrilus (Botaria) flavus Decaëns & Bartz n. sp. in a transitional forest (TF2); G, detail of the terrestrial bromeliad beds in the rocky savannah RS3 (all photos by T. Decaëns).

FIG. 10. — Martiodrilus (Botaria) tchoukouchipan Decaëns & Bartz n. sp.: A, external and lateral view of the anterior body (arrows indicate the clitellum position); B, external and ventral view of the anterior body (arrows indicate the position of the tubercula pubertatis); C, dorsal dissection showing anterior internal anatomy;D, habitat preferences;E, microhabitat preferences.All images from the holotype; CG, calciferous glands;CL, clitellum;GZ, gizzard;SP, spermathecae; SV, seminal vesicles; TF, typhlosole; TP, tubercula pubertatis; N, total number of specimens in the dataset. Scale bars: 5 mm.