Akanthomyces noctuidarum Aini, Luangsa-ard, Mongkolsamrit & Thanakitpipattana, 2020

Aini, Arifah Nur, Mongkolsamrit, Suchada, Wijanarka, Wijanarka, Thanakitpipattana, Donnaya, Luangsa-ard, J. Jennifer & Budiharjo, Anto, 2020, Diversity of Akanthomyces on moths (Lepidoptera) in Thailand, MycoKeys 71, pp. 1-22 : 1

Akanthomyces noctuidarum Aini, Luangsa-ard, Mongkolsamrit & Thanakitpipattana sp. nov. Figure 2

Type.

Thailand. Narathiwat Province, Hala Bala Wildlife Sanctuary, Headquarter Nature Trail; 5°928'N, 101°883'E; on adult moth; 3 Mar 2009; K. Tasanathai (KT), P. Puyngain (PP), T. Chohmee (TC) (holotype BBH 26019 dried culture; ex-type living culture BCC 36265). GenBank: ITS = MT356072, LSU = MT356084, TEF = MT477978, RPB1 = MT477994, RPB2 = MT477987.

Etymology.

Referring to the host ( Noctuidae , Lepidoptera ) where the fungus was found.

Description.

Teleomorph: Adult moth attached to the midrib of monocotyledonous leaf or undersides of dicotyledonous leaf covered by white to cream mycelium (OAC816). Stroma arising from host body and wing veins, white to cream, cylindrical, length ca. 5 mm. Perithecia superficial, orange to light brown (OAC825), few to numerous, crowded at the tip of the stroma or growing directly from mycelium in host body and wing veins, ovoid, (530-)623-993(-1000) × (290-)308-413(-425) µm. Asci cylindrical, hyaline, (170-)196-423(-550) × (2-)2.7-3.8(-4) µm. Ascospores cylindrical, filiform, hyaline, multi-septate, breaking into one-celled fragments at maturity, (6-)7-10.7(-13) × 1 µm.

Anamorph: Synnemata arising from moth body and wing veins, white to cream (OAC816), erect, simple, cylindrical to clavate, (650-)668-1191(-1500) × (50-)53.4-102(-120) µm. Conidiogenous cells produced along the synnemata, monophialidic or polyphialidic. Phialides cylindrical with papillate end, hyaline, (5-)6.8-9(-10) × (1.8-) 2-2.4(-3) µm. Conidia cylindrical with round end, hyaline, (3-)3.5-4.5(-6) × 1 µm.

Culture characters.

Colony on PDA growing with a diameter of 20-24 mm in 14 days, circular, flat to raised, entire edges, white (OAC909) and fluffy mycelium. Colony reverse cream (OAC814). Colony on OA growing with a diameter of 20-25 mm in 14 days, circular, flat to raised, entire, white (OAC 909) and fluffy mycelium. Colony reverse uncolored. Conidia and reproductive structures not observed on both, PDA and OA in 14 days.

Distribution.

Thailand, known from various national parks throughout the country.

Ecology.

All specimens were found on the underside of leaves of plants.

Additional specimens examined.

Thailand. Nakhon Ratchasima Province, Khao Yai National Park, Km.29; 14°711'N, 101°421'E; on adult moth; 24 Jan 2006; KT, W. Chaygate (WC), S. Sivichai, Le Tan Hung (BBH16595). Narathiwat Province, Hala Bala Wildlife Sanctuary, Headquarter Nature Trail; 5°928'N, 101°883'E; on adult moth; 19 Feb 2011; KT (BBH30267, BCC 47498). Kamphaeng Phet Province, Khlong Lan National Park, Saphan Ton Nature Trail; 16°203'N, 99°321'E; on adult moth; 6 Nov 2007; BT, KT, WC, S. Mongkolsamrit (SM), P. Srikitikulchai (PS), R. Ridkaew (RR), A. Khonsanit (AK) (BBH22738, BCC 28571).

Notes.

This species produced both, anamorph and teleomorph. The type strain of this species, BBH 26019/ BCC 36265, consisted of both, anamorph and teleomorph. The other strains produced only one morph on the insect, either anamorph or teleomorph.

Figures

Figure 2. Akanthomyces noctuidarum (BBH 26019, BCC 36265) A, B fungus on adult moth C-F perithecia G asci H tip of ascus I ascus with ascospores J ascospores with clear septae K ascospores break into part-spores L-Q synnemata R-T phialides through the length of synnema U phialides at the tip of synnema V conidia W, X culture on PDA 14 days X reverse Y, Z culture on OA 14 days Z reverse. Scale bars: 1 cm (A, B, W, X, Y, Z); 5 mm (C, I, J, K); 1 mm (D, E, L); 200 µm (F, M, N, O, P, Q); 50 µm (G); 10 µm (H, R, S, T, U, V).