Holtermanniella wattica NH

Park, Yuna & Srinivasan, Soohyun Maeng and Sathiyaraj, 2023, Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter, Journal of Species Research 12 (3), pp. 197-202 : 201

Description of Holtermanniella wattica NH 20

Cells are oval shaped and budding is polar ( Fig. 1 ). Colonies are convex, smooth, and white-colored after 3 days of incubation on YM agar at 10℃. In the API 20C AUX test, strain NH20 is positive for D- glucose, calcium, 2-keto-D- gluconate, L- arabinose, D- xylose, inositol, D- sorbitol, N -methyl-D- glucoside, D- cellobiose, D- lactose (bovine origin), D- maltose, D- saccharose (sucrose), D- trehalose, and D- raffinose; weak positive for glycerol, adonitol, xylitol, and D- galactose; and negative for N -acetyl-D- Glucosamine.

Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791.

Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.

Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger data dets. Mol Biol Evol 33(7):1870-1874.

Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371.

Strain NH 20 ( KCTC 37091 ) was isolated from the soil collected in Namhansanseong Forest , Gwangju City, Gyeonggi Province, Republic of Korea .

Figures

Fig. 1. Morphology of cells from the unrecorded strains incubated at 10℃. The colonies of Leucosporidium scottii NH19(A), Holtermanniella wattica NH20 (B), Buckleyzyma aurantiaca NH33 (C), and Mrakia aquatica YP416(D). The budding cells of Leucosporidium scottii NH19(F), Holtermanniella wattica NH20 (G), Buckleyzyma aurantiaca NH33 (H), and Mrakia aquatica YP416 (I). Bars, 20 μm.All strains were grown after 3 days on YM agar.