Schizomus loreto Armas, 1977: 5–7

Zawierucha, Krzysztof, Szymkowiak, Paweł, Dabert, Miroslawa & Harvey, Mark Stephen, 2013, First record of the schizomid Stenochrus portoricensis (Schizomida: Hubbardiidae) in Poland, with DNA barcode data, Turkish Journal of Zoology 37 (3), pp. 357-361 : 359

Schizomus loreto Armas, 1977: 5–7 , figures 3, 4a–d (synonymized by Armas, 1989: 23).

Full synonymy to 2002 can be found in Harvey, 2003: 125.

Specimens examined: POLAND: Poznań Palm House (pavilions: V, VI, and VIII), January–April 2012, 18 specimens (11 females and 7 juveniles) in AMUP and 6 specimens (1 female and 5 juveniles) in WAM .

Diagnosis: Stenochrus portoricensis differs from all other species of the genus, except S. davisi (Gertsch, 1940) , S. guatemalensis (Chamberlin, 1922) , S. leon Armas, 1995 , S. mexicanus ( Rowland, 1971) , S. mulaiki (Gertsch, 1940) , S. palaciosi ( Reddell and Cokendolpher, 1986) , S. pecki (Rowland, 1973) , and S. reddelli ( Rowland, 1971) , by the presence of only 2 pairs of dorsal propeltidial setae. Females can be distinguished from those of these species by the heavily sclerotized median spermathecae, which are visible through the cuticle of sternite II, and males by seta dm4 of the flagellum being situated on approximately the same level as dl1 ( Rowland and Reddell, 1980; Reddell and Cokendolpher, 1986; Armas, 1995).

Sequence data: We sequenced a 681-bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene (DNA barcode region chosen by the Consortium for the Barcode of Life, http://barcoding.si.edu) and an 850-bp fragment of the nuclear 28S rDNA, including the hypervariable D1–D3 regions of 3 females of S. portoricensis (GenBank Acc. numbers JX280413–15, JX280416, respectively). No intraspecific variation was found in the COI nucleotide sequences, and no frame shift was observed after conversion of these sequences into amino acids. Sequence data for S. portoricensis have been previously reported for several gene regions (e.g., Giribet and Ribera, 2000; Giribet et al., 2002; Regier et al., 2010), but our data represent the first records of DNA barcodes for this species: a fragment of COI from mitochondrial DNA, and hypervariable regions D1–D3 from 28S ribosomal RNA gene. The sequences generated for S. portoricensis showed nucleotide identities higher than 80% (COI) and 90% (28S rDNA) compared to the other schizomid sequences published in GenBank. One or both of these genes have been obtained for other schizomids including Hubbardia pentapeltis Cook, 1899 ( Giribet et al., 2002), Bamazomus sp. , Brignolizomus woodwardi (Harvey, 1992) , Draculoides spp. and Paradraculoides spp. ( Harvey et al., 2008), and an unidentified schizomid (Arabi et al., 2012).