Yamadamyces terricola Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 125-126

Yamadamyces terricola Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828847. Figs 17G and 18H .

Etymology: the specific epithet terricola refers to the substrate from which the type strain was isolated, soil.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are fusiform, 2.5–3.4 × 6.0– 11.8 μm and single, budding is polar ( Fig. 18H ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, trehalose, melezitose, D-glucosamine (delayed and weak), ethanol, glycerol (weak), ribitol, D-mannitol, D-glucitol and succinate (delayed and weak) are assimilated as sole carbon sources. Galactose, L-sorbose, cellobiose, lactose, melibiose, raffinose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, Dribose, L-rhamnose, N-Acetyl-D-glucosamine, methanol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, L-lysine (weak), ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is postive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ya. terricola differs from its closely related species Ya. rosulatus in its inability to assimilate cellobiose, Lrhamnose, N-Acetyl-D-glucosamine, salicin, D-gluconate, DLlactate, citrate, myo-inositol, potassium nitrate and sodium nitrite and its ability to growin vitamin-free medium ( Table S1.32 ).

Typus: China, Daxinganling, obtained from soil, Aug. 2015, Q.- M. Wang (holotype CGMCC 2.5820 T preserved in a metabolically inactive state, ex-type CBS 15572 = 03-1).

Note: The genus Yamadamyces was invalidly published because its type species was based on an invalid name (Art. 40.1, ICN Shenzhen Code),thus it was validated in the Validated Taxa section.

Figures

Fig. 17. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A, B) Ros. cystopteridis CGMCC 2.2615T, A Bars = 10 μm, B Bars = 2 μm; (C) Ros. motuoensis CGMCC 2.5819T, Bars = 10 μm; (D) He. tridentata CGMCC 2.5602T, Bars = 10 μm; (E, F) He. cylindrica CGMCC 2.5650T, E Bars = 20 μm, F Bars = 5 μm; (G) Ya. terricola CGMCC 2.5820T, Bars = 10 μm; (H) Ch. rhododendri CGMCC 2.5821T, Bars = 5 μm.

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.