Yurkovia longicylindrica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 131

Yurkovia longicylindrica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828860. Fig. 19K, L .

Etymology: the specific epithet longicylindrica refers to the elongate cylindrical cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are elongate cylindrical, 2.5 – 4.5 × 7.5 – 15.9 μm and single, budding is polar ( Fig. 19K ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, wrinkled and dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid, falcate or cylindrical, 1.4 – 2.6 × 7.1 – 12.9 μm ( Fig. 19L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, trehalose, melibiose, melezitose, inulin, soluble starch (delayed and weak), Darabinose, ethanol, ribitol, D-mannitol and D-glucitol are assimilated as sole carbon sources. L-sorbose, maltose, cellobiose, lactose, raffinose, D-xylose, L-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 22– 23 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Yu. longicylindrica differs from its closely related species Yu. mendeliana and Yu. nerthusi in its inability to assimilate L-sorbose, maltose, L-arabinose, glycerol and succinate and its ability to assimilate melibiose ( Table S1.36 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5603 T preserved in a metabolically inactive state, ex-type CBS 15550 = GPS20.2C3).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.