Bulleribasidium pseudopanici Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 91

Bulleribasidium pseudopanici Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828767. Fig. 8M, N .

Etymology: the specific epithet pseudopanici refers to the similar colony morphology to that of Bulleribasidium panici .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid, 2.3– 5.0 × 3.8 –7.6 μm and single, budding is polar ( Fig. 8M ), a sediment is formed. After 1 mo at 17 °C, a part ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, slightly wrinkled and dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are subglobosal or ellipsoidal, 4.4– 7.4 × 5.9– 7.4 μm ( Fig. 8N ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, melibiose, raffinose, melezitose, D-xylose, Larabinose, D-arabinose, D-ribose, L-rhamnose, galactitol, Dmannitol, D-glucitol (variable), Methyl-α- D-glucoside, salicin and myo-inositol are assimilated as sole carbon sources. L-sorbose, lactose, inulin, soluble starch, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, D-gluconate, DL-lactate, succinate, citrate and hexadecane are not assimilated. Ammonium sulfate, L-lysine, ethylamine hydrochloride, cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Bu. pseudopanici differs from its closely related species Bu. panici in its inability to assimilate L-sorbose, soluble starch, D-glucosamine, erythritol, ribitol, D-gluconate, DL-lactate and succinate and its ability to form starch like compounds ( Table S1.10 ).

Typus: China, Wuzhishan mountain , Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.4024 T preserved in a metabolically inactive state, ex-type CBS 15510 = WZS17.20).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.