Dioszegia maotaiensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 90

Dioszegia maotaiensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828762. Fig. 8G, H .

Etymology: the specific epithet maotaiensis refers to the geographic origin of the type strain, Maotai county, Guizhou.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 3.6– 5.2 × 4.5– 6.2 μm and single, budding is polar ( Fig. 8G ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are are subglobosal to ellipsoidal, 2.4– 3.5 × 3.5 –5.3 μm ( Fig. 8H ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, melibiose, raffinose, melezitose, inulin, soluble starch (delayed and weak), D-xylose, L-arabinose, D-arabinose, L-rhamnose, succinate (delayed and weak) and citrate (delayed and weak) are assimilated as sole carbon sources. L-sorbose, lactose, D-ribose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, Dmannitol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and ethylamine hydrochloride are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, L-lysine and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Di. maotaiensis and the closely related species Di. ovata , Di. kandeliae , Di. zsoltii , Di. catarinoi , Di. takashimae and Di. athyrii can be distinguished from one another. Di. maotaiensis and Di. ovata differ from the other five species in their ability to grow in vitamin-free medium ( Table S1.9 ).

Typus: China, Maotai county, Guizhou province, obtained from a leaf of an unidentified plant, Mar. 2012, Q.-M. Wang (holotype CGMCC 2.4537 T preserved in a metabolically inactive state, ex-type CBS 15516 = GZMT3A9).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.