Sterigmatospora layueensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 111

Sterigmatospora layueensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828811. Figs 11C and 14O .

Etymology: the specific epithet layueensis refers to geographic origin of the type strain, Layue county, Tibet.

the

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.8– 3.5 × 3.8 –5.9 μm and single, budding is polar ( Fig. 14O ), a sediment is present. One or more conidia are produced on each stalk-like conidiophore. Conidiophore is single or multiple, usually cluster on cells. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is pale yellow, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose, sucrose, maltose, cellobiose, trehalose, raffinose, melezitose, D-xylose (variable), Larabinose (variable), ribitol (variable), D-mannitol, D-glucitol, Methyl-α- D-glucoside and salicin (variable) are assimilated as sole carbon sources. Galactose, lactose, melibiose, inulin, soluble starch, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, galactitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 20 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Typus: China, Layue county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5817 T preserved in a metabolically inactive state, ex-type CBS 15649 = XZ100A2B).

Figures

Fig. 11. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A) Fi. mali CGMCC 2.4012T, Bars = 4 μm; (B) Boe. sterigmata CGMCC 2.4539T, Bars = 5 μm; (C) St. layueensis CGMCC 2.5817T, Bars = 5 μm; (D) Pse. motuoensis CGMCC 2.5816T, Bars = 2 μm; (E) Me. layueensis CGMCC 2.5818T, Bars = 5 μm; (F) Beg. foliicola CGMCC 2.3164T, Bars = 1 μm; (G, H) Ros. petaloides CGMCC 2.3446T, G Bars = 10 μm, H Bars = 3 μm.

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.