Ruinenia fanjingshanensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 109

Ruinenia fanjingshanensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828804. Fig. 14G, H .

Etymology: the specific epithet fanjingshanensis refers to the geographic origin of the type strain, Fanjingshan Mountain, Guizhou.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and cylindrical, 2.1–3.6 × 5.0– 7.9 μm and single, budding is polar ( Fig. 14G ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pink-red, butyrous, wrinkled and dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.1– 3.6 × 5.0– 7.9 μm ( Fig. 14H ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, maltose, trehalose, melibiose, raffi- nose, inulin, soluble starch (weak), ribitol and D-mannitol are assimilated as sole carbon sources. Galactose, L-sorbose, sucrose, cellobiose, lactose, melezitose, D-xylose, L-arabinose, Darabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, galactitol, Dglucitol, Methyl-α- D-glucoside, salicin, D-gluconate, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite and L-lysine are not assimilated. Maximum growth temperature is 21 °C. Growth in vitamin-free medium is negative Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ru. fanjingshanensis differs from its closely related species Ru. dracophylli in its inability to assimilate Lsorbose, sucrose, maltose, cellobiose, melezitose, glycerol, ribitol, galactitol, D-mannitol, D-glucitol, salicin and succinate and its ability to assimilate trehalose, inulin, ethylamine and cadaverine ( Table S1.20 ).

Typus: China, Fanjingshan Mountain , Guizhou province, obtained from a leaf of an unidentified plant, Oct. 2011, Q.-M. Wang (holotype CGMCC 2.4542 T preserved in a metabolically inactive state, ex-type CBS 15745 = FJS6C7).

Figures

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.