Methylobacterium isbiliense

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 140-141

Description of Methylobacterium isbiliense 17SD2_13

Cells are Gram-stain-negative, non-flagellated, and short rod-shaped. Colonies are pink-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III acetoacetic acid, N -acetyl-D-mannosamine, L-alanine, L-arginine, L-aspartic acid, D-cellobiose, dextrin, D-fructose, D-fructose 6-PO 4, L-galactonic acid lactone, D-galactose, D-galacturonic acid, gelatin, gentiobiose, D-gluconic acid, α -D-glucose, glucuronamide, D-glucuronic acid, L-glutamic acid, inosine, α -keto-glutaric acid, L-malic acid, D-maltose, D-mannitol, D-mannose, D-melibiose, β -methyl-D-glucoside, 3-methyl glucose, myo-inositol, pectin, propionic acid, glycyl-L-proline, L-pyroglutamic acid, quinic acid, D-raffinose, L-rhamnose, L-serine, stachyose, sucrose, D-trehalose, D-turanose, acetic acid, N -acetyl-D-galactosamine, N -acetyl-neuraminic acid, N -acetyl-D-glucosamine, γ -amino-butryric acid, D-arabitol, D-aspartic acid, bromo-succinic acid, citric acid, formic acid, D-fucose, L-fucose, D-glucose-6-PO 4, glycerol, L-histidine, α -hydroxybutyric acid, β -hydroxy-D, L-butyric acid, p -hydroxy-phenylacetic acid, α -keto-butyric acid, L-lactic acid, D-lactic acid methyl ester, α -D-lactose, D-malic acid, methyl pyruvate, mucic acid, D-saccharic acid, D-salicin, D-serine, D-sorbitol, and tween 40 were not utilized. In sensitivity tests, the tetrazolium redox dye is reduced in the presence of tetrazolium violet, tetrazolium blue, and aztreonam; but not in the presence of 1% NaCl, 1% sodium lactate, 4% NaCl, 8% NaCl, guanidine HCl, lithium chloride, pH 6, potassium tellurite, D-serine, sodium butyrate, fusidic acid, lincomycin, minocycline, nalidixic acid, niaproof 4, pH 5, rifamycin SV, sodium bromate, troleandomycin, and vancomycin.

In API 20NE system, positive for the reduction of nitrates (NO 3) to nitrite (NO 2 -), arginine dihydrolase, urease, esculin hydrolysis, β -galactosidase, D-glucose, L-arabinose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, D-maltose, potassium gluconate, adipic acid, malic acid, trisodium citrate, and phenylacetic acid. Weak positive for gelatin hydrolysis. Negative for the reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, and capric acid.

In API 32GN system, positive for L-rhamnose, D-ribose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, potassium 2-ketogluconate, 3-hydroxybutyric acid, L-serine, D-glucose, L-arabinose, propionic acid, capric acid, valeric acid, trisodium citrate, 4-hydroxybenzoic acid, L-proline, and 3-hydroxybenzoic acid. Negative for N -acetyl-glucosamine, inositol, D-saccharose (sucrose), D-maltose, L-alanine, potassium 5-ketogluconate, glycogen, L-serine, D-mannitol, salicin, D-melibiose, D-fucose, D-sorbitol, and L-histidine. Strain 17SD2_13 (= NIBRBAC000500516) was isolated from a soil sample.