Periconia epilithographicola Coronado-Ruiz, Avendaño, Escudero-Leyva, Conejo-Barboza, P. Chaverri & Chavarría,

Tian, Xingguo, Tibpromma, Saowaluck, Karunarathna, Samantha C., Dai, Dongqin, Bao, Danfeng, Mapook, Ausana & Jayawardena, Ruvishika S., 2022, Additions to the genus Periconia from northern Thailand, Phytotaxa 555 (1), pp. 73-86 : 79

Periconia epilithographicola Coronado-Ruiz, Avendaño, Escudero-Leyva, Conejo-Barboza, P. Chaverri & Chavarría,

Scientific Reports 8 (no. 7492): 3 (2018). Fig. 3

Index Fungorum number: IF 825093; Facesoffungi number: FoF 10660

Saprobic on dead leaves of Ananas comosus . Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies effuse on natural substrate, scattered, hairy, dark brown to dark. Mycelium composed of cottony, unbranched hyphae, forming dark clusters with conidia. Conidiophores (120–)140–180(–205) × 5–7 μm (x̅ = 159 × 6 µm, n = 40), macronematous, mononematous, unbranched, caespitose, erect, straight, brown to dark brown throughout, septate, smooth-walled. Conidiogenous cells 8−12 × 5−7 μm (x̅ = 10 × 6 μm, n = 20), mono- to polyblastic, determinate, discrete on stipe, intercalary, smooth to minutely verruculose, subglobose to ellipsoid, brown. Conidia 9−11 μm diam. (x̅ = 10 μm, n =45), subglobose to globose, aseptate, pale brown to dark brown, arising at one or more points on the curved surface of the conidiogenous cell, in branched chains, bud scars or disjunctors present at site of attachment, smooth-walled to verruculose.

Culture characteristics: —Conidia germinated on PDA within 12 hr, reaching 25 mm diam. in 2 weeks at 25 °C. Colonies on PDA with sparse, white - orange mycelia on surface, cottony, circular, and flattened; reverse of colony yellow - orange with white margin. Conidiophores and conidia not observed in mature colonies.

Material examined: — THAILAND. Chiang Rai Province: on dead leaves of Ananas comosus , 1 August 2020, X.G. Tian, P6−1 (MFLU 21−0281), living culture MFLUCC 21−0153 .

Notes: —In the phylogenetic analyses, our new collection (MFLUCC 21−0153) clustered with the ex-type strain of P. epilithographicola (CBS 144017) with high statistical supports (100% ML/1.00 PP, Fig. 1 ). Morphologically, our new isolate is identical to P. epilithographicola (CBS 144017) except for longer conidiophores the ex-type (252–270 µm). Nucleotide comparisons showed that there is 1 bp out of 554 bp in ITS sequence difference between the new isolate and P. epilithographicola (CBS 144017). Thus, we identified the new isolate as P. epilithographicola based on phylogenetic analyses and morphological characteristics.

Periconia epilithographicola was originally isolated from 19 th century art lamina in Costa Rica ( Coronado-Ruiz et al. 2018), while the new isolate was collected from Ananas comosus in Thailand. Therefore, P. epilithographicola (MFLUCC 21−0153) is growing on a new host and is a new geographic record.

Figures

FIGURE 1. Phylogenetic tree generated from a maximum likelihood analysis based on a concatenated alignment of ITS, LSU, SSU, and TEF1-α sequences data in Periconiaceae. The tree is rooted with Morosphaeria ramunculicola (KH220) and M. velatispora (KH221). Bootstrap support values equal to or higher than 70% ML (left) or posterior probability values equal to or higher than 0.90 Bayesian PP (right) are indicated on the nodes. The newly generated sequences are in red, and type strains are in black/red bold.

FIGURE 3. Periconia epilithographicola (MFLU 21−0281). a–c. Colonies on natural substrate. d–g. Conidiophores, conidiogenous cells and conidia. h–l. Conidiogenous cells and conidia. m–r. Conidia. s. Colonies on PDA from above and below. Scale bars: d–e = 100 μm, f–h, j–l = 40 μm, i = 20 μm, m–r = 10 μm.