Lambertella pyrolae, Zhao & Hosoya & Shirouzu & Kakishima & Yamaoka, 2013

Zhao, Yan-Jie, Hosoya, Tsuyoshi, Shirouzu, Takashi, Kakishima, Makoto & Yamaoka, Yuichi, 2013, Lambertella pyrolae (Rutstroemiaceae, Ascomycota), a new species from Japan, Phytotaxa 136 (1), pp. 54-60 : 55-59

Lambertella pyrolae Y.-J. Zhao & T. Hosoya, sp. nov. ( Figs. 1−3 )

MycoBank no.:— MB 803966

Holotype: — Japan, Nagano Pref., Ueda City, Sugadaira Montane Research Center of University of Tsukuba, 36º31'32.10"N, 138º20'52.00"E, elev. 1340 m, 23 June 2012, coll. Y.- J. Zhao, TNS-F-40132!, Culture NBRC- 109610.

Distribution and habitat: — Japan, collected from the decayed leaves and petioles of P. incarnata .

Etymology:— named after the host plant genus, Pyrola .

Additional specimens examined: — Japan, Nagano Pref., July 2006, TNS-F-25246 !; Japan, Nagano Pref., 2 July 2011, TNS-F-40033! (Culture NBRC-109611) .

Description:— Stroma substratal, visible on surface of substrate as clear, irregular, black lines delimiting blackened zones. Apothecia stipitate, occurring on decaying leaves and petioles; disc flat when fresh, becoming discoid to cupulate when dry, 0.5–1.6 mm in diameter in dried specimen; hymenium dark gray to beige (warm gray 3C = R199 G194 B186) when fresh, becoming dark yellow to brown (4745PC = C4 M20 Y22 K12) when dry; receptacle hairy, slightly paler than hymenium when fresh, becoming light brown when dry; stipe concolorous with the receptacle, 0.5–2 mm long when dry, with hairy surface. Ectal excipulum two layered: outer layer textura prismatica, composed of thin-walled, brick-shaped cells 15–40 × 6–13 µm in the middle flanks and 6–15 × 5–10 µm at the margin, with granulate or smooth surface, sometimes becoming pale brown towards the margin; inner layer composed of thin-walled, subhyaline to pale brown, granulate or smooth, hypha of ca. 5 µm wide. Hairs arising from the outermost layers of the ectal excipulum, cylindrical, septate, 17–60 µm long, mostly hyaline, occasionally expanded up to 4–8 µm at the apex. Medullary excipulum textura intricata, composed of hyaline, smooth, loosely interwoven hyphae 3–5 µm wide. Asci 81– 145 × 6–9.5 µm (109.7±18.5 × 7.2± 0.9 µm on average ± SD, n = 20), clavate, 8-spored, arising from simple septa; apex rounded, some slightly truncate, 2–4 µm thick; pore never stained by Melzer’s reagent with or without 3% KOH pretreatment; both pigmented and colorless ascospores can be seen in the same ascus, most asci collapsing when all the ascospores have become pigmented. Ascospores 14–22 × 3–4.5 µm (18 ± 2×3.5 ± 0.5 µm on average ± SD, n = 30), irregularly biseriate or biseriate above and uniseriate below, elongateelliptic to fusoid, non-septate; at first hyaline then changing to pale brown, finally becoming yellow-brown to golden-brown within the ascus; hyaline spores smooth, 1–multi guttulate; pale brown spores 1–3 guttulate, remaining smooth or becoming somewhat granulate; brown spores eguttulate, with coarsely granulate surface, some with one side banded. Paraphyses straight, septate, hyaline, simple or branched near the base, expanded at the apex up to 2–5 µm wide.

Germination of ascospores on PDA: germinated spores becoming paler colored to hyaline, expanded at the middle, 7–14 µm in width, aseptate, ends pointed.

Cultural characteristics: Growth on PDA slow, attaining a diameter of 50 mm in 14 d at 20°C, surface floccose, whitish to pale brown (4755PC = C3 M14 Y16 K7), becoming darker with age. Aerial mycelium white, becoming brown with age, not well developed, forming short mycelial strands. Rind becoming distinct in prolonged incubation up to 1 month. The rind delimiting irregular portions of the agar, composed of a single layer of cells with walls pigmented to a various extent, epidermoid in face view.

Figures

FIGURE 1. Lambertella pyrolae (TNS-F-40132). A: Fresh apothecia on Pyrola leaves. B: Dried apothecia in a higher magnification. C: Vertical section of an apothecium. D: Structure of ectal excipulum at the margin. E: Asci showing the pigmented and hyaline ascospores within asci. F: Paraphyses. G: Hairs. H: Pigmented (pale brown and brown) ascospores. I: Germination of ascospores. C– I, mounted in lactic acid. Bars B 1mm; C 200 µm; D–I 20 µm.

FIGURE 2. Morphology of Lambertella pyrolae (TNS-F-40132). A: Vertical section of an apothecium through the margin showing the ectal excipulum. B: Asci. C: Paraphyses. D: Hairs. E: Hyaline and brown ascospores. One at the bottom right shows the banded ornament. F: Germination of ascospores. Line drawings prepared in lactic acid mount.

FIGURE 3. Lambertella pyrolae (NBRC-109610, Culture of TNS-F-40132). A: Colony on PDA (20°C, 14 days). B: Rind on PDA (20°C, 1 month). C: Vertical view of rind. D: Surface view of rind showing the epidermoid cells. Bars C, D 20µm.