Dorcadion lugubre

Dascălu, Maria-Magdalena, Caba, Florina-Georgiana & Fusu, Lucian, 2022, DNA barcoding in Dorcadionini (Coleoptera, Cerambycidae) uncovers mitochondrial-morphological discordance and the hybridogenic origin of several subspecies, Organisms Diversity & Evolution (New York, N. Y.) 22 (1), pp. 205-229 : 224

Dorcadion lugubre and D. lineatocolle

The COI sequences of D. lugubre are divided between two clades, with one sequence grouped with D. lineatocolle . This misplaced specimen is small for its species, about the same size as D. lineatocolle ( Fig. 7 ). It is heterozygous for the ITS2 sequence, having a haplotype that groups with D. lugubre and one that groups with D. lineatocolle (Figs. 1, S2 and 7). According to Danilevsky (2014), specimens of D. lugubre from Struma Valley in Bulgaria are to be attributed to D. lugubre minkovae . Our specimen of D. lugubre with D. lineatocolle mitochondrial haplotype and with an ITS2 sequence of both species comes precisely from this area (Promachonas Village in Greece, just before the river Struma enters Roupel Gorge). Hence, based on morphology and molecular data, the specimen linPro1001 is actually a hybrid between D. lugubre lugubre or D. lugubre minkovae and D. lineatocolle , a species present in the area ( Minkova, 1961). A comprehensive sampling (like the one undertaken for D. pusillum , D. axillare and D. murrayi ) is needed to confirm this hypothesis.

Figures

Fig. 7 Habitus of voucher specimens (from left to right): Dorcadion lugubre lugubre (luKe1001 and luKe0801), D. lugubre × D. lineatocolle (linPro1001) and D. lineatocolle (linAng1001). All at the same