Diaporthe schisandrae C.M. Tian & Q. Yang, 2018

Yang, Qin, Du, Zhuo & Tian, Cheng-Ming, 2018, Phylogeny and morphology reveal two new species of Diaporthe from Traditional Chinese Medicine in Northeast China, Phytotaxa 336 (2), pp. 159-170 : 165

Diaporthe schisandrae C.M. Tian & Q. Yang , sp. nov. FIGURE 3

MycoBank no: MB823870

Holotype:— BJFC-S1370 .

Etymology:— schisandrae : named after the host genus, Schisandra .

Host/Distribution:—from Schisandra chinensis in northeast China.

Original description:—Sexual state: Undetermined. Asexual morph: Conidiomata pycnidial, globose to ovoid, embedded in bark, erumpent through the bark surface at maturity, sparse, with a single locule. Ectostromatic disc (160)250–400(−500) μm (av. = 325 μm, n = 20), black, one ostiole per disc. Locule undivided, (320)400–700(−750) μm (av. = 575 μm, n = 20) in diam. Wall parenchymatous, consisting 3–4 layers of medium brown textura angularis. Conidiophores hyaline, unbranched, phialides, cylindrical, tapering towards the apex, (10.5–)13.5–20(−22) × (1.4–)1.7−2(−2.3) μm (av. = 16.5 × 1.8 μm, n = 50), straight or slightly curved. Conidiogenous cells hyaline, phialides, cylindrical, terminal, slightly tapering towards the apex, 0.5–1 μm diam. Paraphyses absent. Alpha conidia abundant in twigs, (7.5–)8.5–11.5(−12) × (2.5–)2.7−3.3(−3.5) μm (av. = 10 × 3 μm, n = 50), hyaline, aseptate, ellipsoidal to fusoid, somewhat tubercular at one end and obtuse at other, 1–3-guttulate. Beta conidia not seen.

Culture characters:—Cultures incubated on PDA at 25 ° C in darkness, colony with white felty aerial mycelium, becoming yellow compact aerial mycelium at the centre, following the white aerial mycelium, and dark brown at the marginal area. Conidiomata dense, distributed in circularity over agar surface.

Material examined:— CHINA, Heilongjiang Province, Yichun city, Dailing District, 47°10’57.63”N, 128°53’35.15”E, 428 m asl, on twigs and branches of Schisandra chinensis, Q. Yang and Z. Du , 27 July 2016 ( BJFC-S 1370, holotype; living ex-type culture, CFCC 51988). Heilongjiang Province, Yichun city, Dailing District, 47°10’57.70”N, 128°53’35.20”E, 430 m asl, on twigs and branches of Schisandra chinensis, Q. Yang and Z. Du , 29 July 2016 ( BJFC-S 1371, paratype; living culture, CFCC 51989).

Notes:—Two isolates of D. schisandrae cluster in a well-supported clade and appeared closely related to D. rostrata and D. juglandicola . Diaporthe schisandrae differs from D. rostrata in smaller locules (400–700 μm in D. schisandrae vs. 620–1100 μm in D. rostrata ) and narrower alpha conidia (2.7–3.3 μm in D. schisandrae vs. 4–5 μm in D. rostrata ) ( Fan et al. 2015); from D. juglandicola in bigger alpha conidia (8.5–11.5 μm in D. schisandrae vs. 8–9 μm in D. juglandicola ) ( Yang et al. 2017b).

Figures

FIGURE 3. Morphology of Diaporthe schisandrae from Schisandra chinensis (BJFC-S1370). A: Habit of conidiomata on branches. B: Longitudinal section of conidioma. C: Transverse section of conidioma. D, E: Conidia. F: Conidiophores. G: Colonies on PDA at 30 days. Scale bars: B–C = 200 μm; D–F = 10 μm.