Flavobacterium tibetense, Phurbu & Lu & Xue & Tian & Pema & Ma & Xing, 2019

Phurbu, Dorji, Lu, Hubin, Xue, Xiaofeng, Tian, Yanling, Pema, Yangzom, Ma, Chao & Xing, Peng, 2019, Flavobacterium tibetense sp. nov., isolated from a wetland, International Journal of Systematic and Evolutionary Microbiology 69 (1), pp. 165-170 : 168-169

DESCRIPTION OF FLAVOBACTERIUM TIBETENSE SP. NOV.

Flavobacterium tibetense (ti.bet.en′ se. N.L. neut. adj. tibetense pertaining to the Tibetan Plateau)

Cells are Gram-stain-negative, non-motile, rod-shaped and occur as single or paired cells. Cells are 1.0–1.6 µm in length and 0.4–0.5 µm in width. Colonies are yellow and circular with entire margins on TSA plates. Growth occurs with 0– 3 % (w/v) NaCl (optimum 1 %), at 10–37 Ǫ C (optimum 30 Ǫ C) and at pH 6–9 (optimum pH 7). Unable to grow on TSA under anaerobic conditions. Oxidase and catalase reactions are positive. Negative for the following: citrate utilization, H 2 S production, indole production and glucose fermentation. Positive for hydrolysis of casein and Voges– Proskauer test. In API 20NE tests, positive for arginine dihydrolase, but negative for arabinose, mannose, N -acetylglucosamine, maltose, gluconate, adipic acid and malic acid. Can produce acid from aesculin. Positive for the enzyme activity of cystine arylamidase, weakly positive for esterase C4, esterase lipase C8 and chymotrypsin, but negative for a-glucosidase. The predominant polar lipid is PE. The predominant respiratory quinone is MK-6. The diamino acid in the cell-wall peptidoglycan is meso -diaminopimelic acid.

The type strain, YH5 T (= CICC 24247 T = KCTC 62174 T), was isolated from the YonghuCo wetland on the Tibetan Plateau. The genomic DNA G+C content of the type strain is 33.3 mol% .